FlyBase Allele Report: Dmel\E2f2[UAS.cFa]

General Information

Symbol

Dmel\E2f2^UAS.cFa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Frolov

FlyBase ID

FBal0126595

Feature type

allele

Associated gene

Dmel\E2f2

Associated Insertion(s)

Carried in Construct

P{UAS-E2f2.F}

Key Links

Transgenic product class

cDNA

(Frolov et al., 2001)

wild_type

(Frolov et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Frolov et al., 2001)

wild_type

(Frolov et al., 2001)

Mutagen

in vitro construct

(Frolov et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-E2f2.F}

Cytology

Description

UASt sequences drive expression of a E2f2 cDNA.

(Frolov et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

E2f2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Frolov et al., 2001)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Frolov et al., 2001)

ommatidium, with Scer\GAL4^GMR.PF

(Frolov et al., 2001)

Detailed Description

Statement

Reference

When E2f2^Scer\UAS.cFa is driven by Scer\GAL4^ap-md544 no visible wing phenotype is seen. When E2f2^Scer\UAS.cFa is driven by Scer\GAL4^ey.PH no visible eye phenotype is seen.

(Stevaux et al., 2002)

E2f2^Scer\UAS.cFa when driven by Scer\GAL4^GMR.PF causes severely abnormal eyes. They are rough in appearance and ommatidia lack their regular pattern. This phenotype is dose dependant.

(Frolov et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

E2f2^UAS.cFa, Scer\GAL4^GMR.PF has visible phenotype, suppressible by E2f1^GMR.PD, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

Other

Statement

Reference

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has abnormal size | larval stage phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has abnormal size | adult stage phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has decreased occurrence of cell division | larval stage phenotype

(Stevaux et al., 2002)

Phenotype Manifest In

Enhanced by

Statement

Reference

E2f2^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by E2f1⁰⁷¹⁷², Scer\GAL4^GMR.PF

(Frolov et al., 2001)

E2f2^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype, enhanceable by E2f1^rM729, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

E2f2^UAS.cFa, Scer\GAL4^GMR.PF has ommatidium phenotype, enhanceable by E2f1^rM729, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

Suppressed by

Statement

Reference

E2f2^UAS.cFa, Scer\GAL4^GMR.PF has eye phenotype, suppressible by E2f1^GMR.PD, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

E2f2^UAS.cFa, Scer\GAL4^GMR.PF has ommatidium phenotype, suppressible by E2f1^GMR.PD, Scer\GAL4^GMR.PF

(Frolov et al., 2001)

Other

Statement

Reference

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ey.PH has eye phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ey.PH has wing disc phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ey.PH has S phase & dorsal mesothoracic disc phenotype

(Stevaux et al., 2002)

E2f2^UAS.cFa, Rbf2^UAS.cSa, Scer\GAL4^ap-md544 has wing phenotype

(Stevaux et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The combination of E2f2^Scer\UAS.cFa and Rbf2^Scer\UAS.cSa driven by Scer\GAL4^ap-md544 leads to a clearly visible wing atrophy phenotype. Wing discs from mutant larvae have phenotypes that vary from being shrunken and abnormal to those that have a relative normal morphology but have many cells that show a decrease in DNA synthesis and have a much higher of proportion of cells in G1 phase, and a lower proportion in S and G2 phases. The combination of E2f2^Scer\UAS.cFa and Rbf2^Scer\UAS.cSa driven by Scer\GAL4^ey.PH leads to a clearly visible small eye phenotype.

(Stevaux et al., 2002)

The addition of E2f^GMR.PD almost completely suppresses the eye phenotypes seen in E2f2^Scer\UAS.cFa, Scer\GAL4^GMR.PF flies. The addition of E2f^rM729 or E2f⁰⁷¹⁷² slightly enhances the eye phenotypes seen in E2f2^Scer\UAS.cFa, Scer\GAL4^GMR.PF flies.

(Frolov et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E2f2^Scer\UAS.cFa

E2f2^UAS.cFa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Frolov

(Frolov et al., 2001)

Secondary FlyBase IDs

References (2)

Report Sections

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