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General Information
Symbol
Dmel\Hsc70-4HS1-25
Species
D. melanogaster
Name
FlyBase ID
FBal0144801
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Hsc70-4R447H
Allele class
Mutagen
    Nature of the Allele
    Allele class
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    G15245699A

    Amino acid change:

    R447H | Hsc70-4-PA; R447H | Hsc70-4-PB; R447H | Hsc70-4-PC; R447H | Hsc70-4-PD; R447H | Hsc70-4-PE; R447H | Hsc70-4-PF; R447H | Hsc70-4-PG

    Reported amino acid change:

    R447H

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: R447H.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    boss protein (as assayed with bossT:Arus\HRP, in somatic clones in the eye is not properly internalized into endosomes. Delivery of bossT:Arus\HRP to the R8 cell surface is not blocked. Generally embryonic lethal when homozygous, though some survive to larval stages though are thin, translucent and developmentally delayed. They often contain melanotic masses and clumps near the brain. Eye discs show the same pattern of boss staining as for homozygous clones. R7 specification is normal. Garland cells of mutant larvae show no uptake of TR-avidin indicating that the internalisation of endocytic traces is completely blocked in mutant cells. Morphology of garland cells is altered:labyrinthine channels are absent, coated vesicles are absent and there is a dramatic increase in multilamellar lysosomal structures.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhancer of
    Statement
    Reference

    Hsc70-4HS1-25/Hsc70-4[+] is an enhancer of visible phenotype of shiK39A.GMR

    Phenotype Manifest In
    NOT suppressed by
    Statement
    Reference

    Hsc70-4HS1-25 has endosome | somatic clone phenotype, non-suppressible by auxGMR.PC

    Enhancer of
    Statement
    Reference

    Hsc70-4HS1-25/Hsc70-4[+] is an enhancer of eye phenotype of shiK39A.GMR

    Hsc70-4HS1-25/Hsc70-4[+] is an enhancer of ommatidium phenotype of shiK39A.GMR

    Additional Comments
    Genetic Interactions
    Statement
    Reference
    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Images (0)
    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    Reported As
    Symbol Synonym
    Hsc70-4HS1-25
    Name Synonyms
    Secondary FlyBase IDs
      References (3)