FlyBase Allele Report: Dmel\tsr[Δ96]

General Information

Symbol

Dmel\tsr^Δ96

Species

D. melanogaster

Name

FlyBase ID

FBal0146730

Feature type

allele

Associated gene

Dmel\tsr

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Blair et al., 2006)

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with tsr^139.cBa, tsr^V27Q.cBa

(Blair et al., 2006)

abnormal cell polarity, with tsr^hs.PB

(Blair et al., 2006)

lethal | larval stage | heat sensitive, with tsr^139.cBa

(Blair et al., 2006)

lethal | larval stage | heat sensitive, with tsr^V27Q.cBa

(Blair et al., 2006)

lethal | pupal stage | cold sensitive, with tsr^139.cBa

(Blair et al., 2006)

lethal | pupal stage | cold sensitive, with tsr^V27Q.cBa

(Blair et al., 2006)

lethal - all die before end of first instar larval stage

(Blair et al., 2006)

lethal - all die before end of larval stage

(Chen et al., 2001)

lethal - all die before end of larval stage | heat sensitive, with tsr^139.cBa, tsr^V27Q.cBa

(Blair et al., 2006)

lethal - all die before end of pupal stage | heat sensitive, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

some die during first instar larval stage

(Blair et al., 2006)

some die during larval stage

(Chen et al., 2001)

some die during larval stage | heat sensitive, with tsr^139.cBa, tsr^V27Q.cBa

(Blair et al., 2006)

some die during pupal stage | heat sensitive, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

viable | cold sensitive, with tsr^139.cBa, tsr^V27Q.cBa

(Blair et al., 2006)

Phenotype Manifest In

adult abdominal sensillum, with tsr^hs.PB

(Blair et al., 2006)

adult optic lobe, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

bract, with tsr^hs.PB

(Blair et al., 2006)

eye, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

lamina, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

leg sensillum, with tsr^hs.PB

(Blair et al., 2006)

microchaeta, with tsr^139.cBa, tsr^V27Q.cBa

(Blair et al., 2006)

ommatidium, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

ommatidium, with tsr^hs.PB

(Blair et al., 2006)

retina, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

rhabdomere, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

rhabdomere membrane, with Pc^G4DBD.GMR, tsr^UAS.int

(Pham et al., 2008)

wing hair, with tsr^139.cBa, tsr^V27Q.cBa

(Blair et al., 2006)

wing hair, with tsr^hs.PB

(Blair et al., 2006)

Detailed Description

Statement

Reference

tsr^Δ96 homozygotes that are carrying both the tsr^UAS.int rescue transgene, and also Pc^G4DBD.GMR (which represses expression of tsr^UAS.int in the eye) have a rough eyes containing black patches of dead necrotic cells. The retina of these flies is significantly thinner than controls. A defect in the optic lobe is seen in some lines, with no clearly defined lamina. The rhabdomeres of these 'stronger phenotype' lines are smaller than normal, exhibiting a disorganised structure and microvilli of variable length. The integrity of the rhabdomere is frequently not maintained, allowing microvilli to 'unravel'. These defects are less severe in some lines, where the length of the individual microvilli are only slightly shorter than normal and do not unravel. However, the rhabdomeres of these intermediate mutants are abnormal in shape, either wider than normal or split in two. The adherens junction of many of the mutant photoreceptor cells are also elongated.

(Pham et al., 2008)

Over 90% of tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 flies raised at 18^oC show planar cell polarity defects. For example, wing hairs have a non-distal orientation. The site of wing prehair initiation is variable in these mutants while initiation occurs at the distal side of cells in wild type. In moderately to severely affected tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 wings, prehairs are observed as F-actin accumulations near cell centres or as long F-actin fibers that span from near cell centres to the distal side of cells. Unlike in wild type, prehairs are not oriented along the PD axis but emerge from either side adjacent to the distal-most vertex of the cell. Additionally, F-actin accumulation on apical cell surfaces is increased and variable compared to wild-type wing cells.

The fine hairs of the notum often adopt non-posterior orientations in tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 flies. Occasionally, multiple notum hairs are observed.

tsr^hs.PB; tsr^Δ96 flies show extensive planar cell polarity defects. In adult wings, the hairs do not uniformly point distally as they do in wild type. In the eye, ommatidia show random chirality and polarity. In the abdomen, fine hairs and bristles frequently have non-posterior orientations. Legs display aberrant tarsal joints and joint duplications and leg bristles often show non-distal orientations. Bracts at the base of bristle show opposite directions; for example, two bracts may have correct polarity but the third may have reversed polarity.

tsr^hs.PB/Y; tsr^Δ96/+ flies appear wild type.

(Blair et al., 2006)

Homozygotes die as first instar larvae.

(Chen et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Pc^G4DBD.GMR, tsr^Δ96, tsr^UAS.int has eye phenotype, enhanceable by ssh^26-1/ssh[+]

(Pham et al., 2008)

Pc^G4DBD.GMR, tsr^Δ96, tsr^UAS.int has eye phenotype, enhanceable by flr[+]/flr^BG2697R5

(Pham et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype found in tsr^Δ96 mutants carrying tsr^Scer\UAS.cPa expressed under the control of Scer\GAL4^GMR.Pc is enhanced in a ssh^26-1 or flr^BG2697R5 heterozygous background.

(Pham et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

tsr^Δ96 is rescued by Pc^G4DBD.GMR/tsr^UAS.int

(Pham et al., 2008)

Partially rescued by

tsr^Δ96 is partially rescued by tsr^139.cBa

(Blair et al., 2006)

tsr^Δ96 is partially rescued by tsr^V27Q.cBa

(Blair et al., 2006)

tsr^Δ96 is partially rescued by tsr^hs.PB

(Blair et al., 2006)

Comments

Expression of tsr^139.cBa results in a limited, temperature-dependent rescue of tsr^Δ96 mutants. At 29^oC, lethality is not rescued at all, at 25^oC, a few tsr^139.cBa; tsr^Δ96 animals survive to the second instar larval stage, and at 18^oC and 16^oC these animals are pupal lethal. The same pattern of rescue is observed with expression of the tsr^V27Q.cBa transgene. Coexpression of both tsr^139.cBa and tsr^V27Q.cBa results in a more effective rescue, although this is still temperature-dependent. While tsr^V27Q.cBa, tsr^139.cBa; tsr^Δ96 animals die at the first larval instar at 29^oC, a few survive to the pupal stage at 25^oC, while animals survive to the adult stage at 16^oC and 18^oC.

Expression of tsr^hs.PB rescues tsr^Δ96 flies following a heat shock. A small number of flies are rescued when there is no heat shock.

(Blair et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tsr^null

(Wu et al., 2016)

tsr^Δ96

(Chu et al., 2012, Pham et al., 2008, Blair et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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