FlyBase Allele Report: Dmel\tsl[UAS.cSa]

General Information

Symbol

Dmel\tsl^UAS.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0150199

Feature type

allele

Associated gene

Dmel\tsl

Associated Insertion(s)

Carried in Construct

P{UAS-tsl.S}

Also Known As

UAS-tsl, UAStsl

Key Links

Transgenic product class

cDNA

(Stevens et al., 2003)

wild_type

(Stevens et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Stevens et al., 2003)

wild_type

(Stevens et al., 2003)

Mutagen

in vitro construct

(Stevens et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-tsl.S}

Cytology

Description

UASt sequences drive expression of a tsl cDNA.

(Stevens et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

tsl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(de las Heras and Casanova, 2006)

embryonic abdomen | maternal effect, with Scer\GAL4^ET3

(Stevens et al., 2003)

embryonic segment | maternal effect, with Scer\GAL4^{VP16.mat.αTub67C}

(de las Heras and Casanova, 2006)

embryonic thorax | maternal effect, with Scer\GAL4^ET3

(Stevens et al., 2003)

Detailed Description

Statement

Reference

Embryos from tsl^Scer\UAS.cSa/+; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} females make cuticle containing head and tail structures but lacking almost all structures of the segmented trunk.

(de las Heras and Casanova, 2006)

When tsl^Scer\UAS.cSa is driven in the follicle cells by Scer\GAL4^ET3, the progeny of these mothers have a strong embryonic phenotype. The head and tail structures are present but the segmented thoracic and abdominal regions of the embryos are disrupted.

(Stevens et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^tj.PU, dally⁸⁰/dally⁰⁶⁴⁶⁴, tsl^UAS.cSa has abnormal cell number | oogenesis phenotype

(Liu et al., 2024)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{VP16.mat.αTub67C}, tsl^UAS.cSa has embryonic segment | maternal effect phenotype, suppressible | maternal effect | partially by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(de las Heras and Casanova, 2006)

Scer\GAL4^{VP16.mat.αTub67C}, tsl^UAS.cSa has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible | maternal effect | partially by Stat92E[+]/Stat92E⁰⁶³⁴⁶

(de las Heras and Casanova, 2006)

NOT Enhancer of

Statement

Reference

Scer\GAL4^ET3/tsl^UAS.cSa is a non-enhancer of cephalopharyngeal skeleton | maternal effect phenotype of fs(1)M3¹⁹⁰¹

(Stevens et al., 2003)

Scer\GAL4^ET3/tsl^UAS.cSa is a non-enhancer of terminalia | maternal effect phenotype of fs(1)M3¹⁹⁰¹

(Stevens et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^ET3/tsl^UAS.cSa is a non-suppressor of terminalia | maternal effect phenotype of fs(1)M3¹⁹⁰¹

(Stevens et al., 2003)

Scer\GAL4^ET3/tsl^UAS.cSa is a non-suppressor of cephalopharyngeal skeleton | maternal effect phenotype of fs(1)M3¹⁹⁰¹

(Stevens et al., 2003)

Other

Statement

Reference

Scer\GAL4^tj.PU, dally⁸⁰/dally⁰⁶⁴⁶⁴, tsl^UAS.cSa has ovary | larval stage phenotype

(Liu et al., 2024)

Additional Comments

Genetic Interactions

Statement

Reference

The segmentation defects seen in the cuticles of embryos from tsl^Scer\UAS.cSa/+; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} mothers are partially but significantly suppressed if the mothers are also Stat92E⁰⁶³⁴⁶/+.

(de las Heras and Casanova, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^tsl.G/tsl^UAS.cSa rescues tsl⁶⁰⁴

(Furriols et al., 2007)

Partially rescues

tsl^UAS.cSa/Scer\GAL4^slbo.2.6 partially rescues tsl⁶⁰⁴

(Furriols et al., 2007)

Scer\GAL4^tsl.F/tsl^UAS.cSa partially rescues tsl⁶⁰⁴

(Furriols et al., 2007)

Comments

Expression of tsl^Scer\UAS.cSa under the control of Scer\GAL4^slbo.2.6 rescues the anterior terminal structure defects seen in tsl⁶⁰⁴ mutant embryos, with 100% of embryos having a dorsal bridge. However, many of the embryos have head involution defects, meaning that only embryos with normal head involution could be examined. The posterior phenotype is also rescued, but not in all embryos.

Expression of tsl^Scer\UAS.cSa under the control of Scer\GAL4^tsl.G rescues the anterior and posterior terminal structure defects seen in tsl⁶⁰⁴ mutant embryos, with 100% of embryos having a dorsal bridge.

Expression of tsl^Scer\UAS.cSa under the control of Scer\GAL4^tsl.F rescues the anterior and terminal structure defects seen in tsl⁶⁰⁴ mutant embryos, with 100% of embryos having a dorsal bridge. The posterior phenotypes are not rescued.

(Furriols et al., 2007)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

92616

P{UAS-tsl.S}2.1; P{white-un4}tsl⁶⁰⁴/TM6B, Tb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tsl^Scer\UAS.cSa

tsl^UAS.cSa

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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