FlyBase Allele Report: Dmel\sxc[6]

General Information

Symbol

Dmel\sxc⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0150409

Feature type

allele

Associated gene

Dmel\sxc

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Gambetta et al., 2009)

Mutagen

ethyl methanesulfonate

(Myster and Peifer, 2003.9.11)

Progenitor genotype

Cytology

Description

Nucleotide substitution of AG to AA destroys the splice acceptor site in the fourth intron of sxc.

(Gambetta et al., 2009)

sxc⁶ is a splice acceptor mutation of AG to AA.

(Sinclair et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:5,320,418..5,320,418 [+]

Nucleotide change:

G5320418A

Reported nucleotide change:

G?A

Comment:

AG to AA mutation in a splice acceptor.

(Gambetta et al., 2009, Sinclair et al., 2009)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile (with sxc²)

(Gambetta et al., 2009)

lethal (with sxc^K872M)

(Mariappa et al., 2018)

lethal | recessive

(Myster and Peifer, 2003.9.11)

lethal - all die before end of larval stage | maternal effect (with sxc¹), with Scer\GAL4^Tub.PU, sxc^D955A.UAS

(Mariappa et al., 2015)

lethal - all die before end of P-stage (with sxc¹)

(Mariappa et al., 2015, Gambetta et al., 2009)

lethal - all die before end of P-stage (with sxc³)

(Gambetta et al., 2009)

lethal - all die before end of P-stage (with sxc⁴)

(Gambetta et al., 2009)

lethal - all die before end of P-stage (with sxc⁵)

(Gambetta et al., 2009)

some die during pharate adult stage (with sxc¹)

(Gambetta et al., 2009)

some die during pharate adult stage (with sxc³)

(Gambetta et al., 2009)

some die during pharate adult stage (with sxc⁴)

(Gambetta et al., 2009)

some die during pharate adult stage (with sxc⁵)

(Gambetta et al., 2009)

viable (with sxc²)

(Gambetta et al., 2009)

visible (with sxc¹), with Scer\GAL4^Tub.PU, sxc^D955A.UAS

(Mariappa et al., 2015)

visible (with sxc¹), with Scer\GAL4^Tub.PU, sxc^H537A.UAS

(Mariappa et al., 2015)

visible (with sxc¹), with Scer\GAL4^Tub.PU, sxc^H596F.UAS

(Mariappa et al., 2015)

visible (with sxc¹), with Scer\GAL4^Tub.PU, sxc^UAS.cMa

(Mariappa et al., 2015)

Phenotype Manifest In

wing (with sxc¹), with Scer\GAL4^Tub.PU, sxc^D955A.UAS

(Mariappa et al., 2015)

wing (with sxc¹), with Scer\GAL4^Tub.PU, sxc^H537A.UAS

(Mariappa et al., 2015)

wing (with sxc¹), with Scer\GAL4^Tub.PU, sxc^H596F.UAS

(Mariappa et al., 2015)

wing (with sxc¹), with Scer\GAL4^Tub.PU, sxc^UAS.cMa

(Mariappa et al., 2015)

wing (with sxc²)

(Gambetta et al., 2009)

wing vein | ectopic (with sxc¹), with Scer\GAL4^Tub.PU, sxc^D955A.UAS

(Mariappa et al., 2015)

wing vein | ectopic (with sxc¹), with Scer\GAL4^Tub.PU, sxc^H537A.UAS

(Mariappa et al., 2015)

wing vein | ectopic (with sxc¹), with Scer\GAL4^Tub.PU, sxc^H596F.UAS

(Mariappa et al., 2015)

wing vein | ectopic (with sxc¹), with Scer\GAL4^Tub.PU, sxc^UAS.cMa

(Mariappa et al., 2015)

Detailed Description

Statement

Reference

sxc⁶ is lethal in combination with sxc^K872M.

(Mariappa et al., 2018)

sxc¹/sxc⁶ transheterozygotes die at pharate adult stage.

Expressing sxc^Scer\UAS.cMa under the control of Scer\GAL4^tub.PU partially rescues the lethality of sxc¹/sxc⁶ transheterozygotes and although the majority of the surviving adults have normal wings, a significant proportion has wings with an ectopic vein; these surviving females have viable progeny.

Expressing either sxc^{H537A.Scer\UAS} or sxc^{H596F.Scer\UAS} under the control of Scer\GAL4^tub.PU partially rescues the lethality of sxc¹/sxc⁶ transheterozygotes but the majority of the surviving adults has wing defects which consist mostly of ectopic veins; the rest have normal wing morphology; these surviving females have viable progeny.

Expressing sxc^{D955A.Scer\UAS} under the control of Scer\GAL4^tub.PU partially rescue the lethality of sxc¹/sxc⁶ transheterozygotes but the majority of surviving adults show wing defects, including ectopic veins, notches and blistering; the progeny of sxc^{D955A.Scer\UAS}-rescued sxc¹/sxc⁶ females dies before reaching second larval instar (crossed with either sxc^{D955A.Scer\UAS}-rescued sxc¹/sxc⁶ males) or adulthood (crossed with wild-type males) due to maternal effect.

(Mariappa et al., 2015)

Flies trans-heterozygous for sxc⁶ and sxc¹, sxc³, nsxc⁴ or sxc⁵ develop into pharate adults showing a typical sxc mutant phenotype.

sxc²/sxc⁶ flies are viable and fertile, but exhibit wing abnormalities suggestive of wing-to-haltere transformations.

(Gambetta et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

sxc⁵

(Gambetta et al., 2009)

sxc⁴

(Gambetta et al., 2009)

sxc³

(Gambetta et al., 2009)

sxc¹

(Gambetta et al., 2009)

Partially rescued by

sxc⁶/sxc¹ is partially rescued by Scer\GAL4^Tub.PU/sxc^UAS.cMa

(Mariappa et al., 2015)

sxc⁶/sxc¹ is partially rescued by Scer\GAL4^Tub.PU/sxc^H537A.UAS

(Mariappa et al., 2015)

sxc⁶/sxc¹ is partially rescued by sxc^H596F.UAS/Scer\GAL4^Tub.PU

(Mariappa et al., 2015)

sxc⁶/sxc¹ is partially rescued by sxc^D955A.UAS/Scer\GAL4^Tub.PU

(Mariappa et al., 2015)

Not rescued by

sxc⁶/sxc¹ is not rescued by Scer\GAL4^Tub.PU/sxc^K872M.UAS

(Mariappa et al., 2015)

sxc⁶/sxc¹ is not rescued by sxc^N948I.UAS/Scer\GAL4^Tub.PU

(Mariappa et al., 2015)

Comments

(Mariappa et al., 2015)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

7182

sxc⁶ cn¹ bw¹/SM1

50890

w^*; Eogt^ex10 sxc⁶/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

Notes on Origin

Discoverer

Induced on: cn¹ bw¹.

(Myster and Peifer, 2003.9.11)

Comments

One of 6 alleles of this locus identified.

(Myster and Peifer, 2003.9.11)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

l(2)NC130

(Gambetta and Müller, 2009.8.18, Gambetta et al., 2009)

l(2)NC130^NC95

sxc⁶

(Mariappa et al., 2018, Mariappa et al., 2015, Radermacher et al., 2014, Müller et al., 2013, Na et al., 2013, Mariappa et al., 2011, Gambetta and Müller, 2009.8.18, Gambetta et al., 2009)

sxc^NC130

(Sinclair et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (11)

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