FlyBase Allele Report: Dmel\ed[2B8]

General Information

Symbol

Dmel\ed^2B8

Species

D. melanogaster

Name

FlyBase ID

FBal0150514

Feature type

allele

Associated gene

Dmel\ed

Associated Insertion(s)

P{lacW}ed^2B8

Carried in Construct

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Chandra et al., 2003)

Mutagen

Progenitor genotype

Associated Insertion(s)

P{lacW}ed^2B8

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Df(2L)ed1)

(Ahmed et al., 2003)

abnormal neuroanatomy (with ed^3C2)

(Ahmed et al., 2003)

abnormal neuroanatomy (with ed^m1)

(Ahmed et al., 2003)

abnormal neuroanatomy (with ed^ts)

(Ahmed et al., 2003)

abnormal neuroanatomy | recessive

(Ahmed et al., 2003)

lethal (with ed^m1)

(Ahmed et al., 2003)

lethal | heat sensitive (with ed^ts)

(Ahmed et al., 2003)

lethal - all die during embryonic stage | recessive

(Ahmed et al., 2003)

some die during embryonic stage | recessive

(Ahmed et al., 2003)

Phenotype Manifest In

embryonic neuroblast | ectopic

(Ahmed et al., 2003)

eye (with ed^ts)

(Ahmed et al., 2003)

larval neuron | increased number

(Ahmed et al., 2003)

larval ventral nerve cord (with ed^ts)

(Ahmed et al., 2003)

macrochaeta | ectopic (with ed^ts)

(Ahmed et al., 2003)

microchaeta (with ed^ts)

(Ahmed et al., 2003)

presumptive embryonic/larval central nervous system

(Ahmed et al., 2003)

presumptive embryonic/larval central nervous system (with Df(2L)ed1)

(Ahmed et al., 2003)

presumptive embryonic/larval central nervous system (with ed^3C2)

(Ahmed et al., 2003)

presumptive embryonic/larval central nervous system (with ed^m1)

(Ahmed et al., 2003)

presumptive embryonic/larval central nervous system (with ed^ts)

(Ahmed et al., 2003)

wing margin | distal (with ed^ts)

(Ahmed et al., 2003)

wing vein L2 (with ed^ts)

(Ahmed et al., 2003)

Detailed Description

Statement

Reference

Embryonic lethal with ed^2B8 at 29^oC. Homozygous, ed^2B8/Df(2L)ed1, ed^2B8/ed^m1 and ed^2B8/ed^3C2 embryos show hyperplasia of the central nervous system. ed^2B8/ed^ts embryos grown at the restrictive temperature of 29^oC show hyperplasia of the central nervous system, resulting in local bulging of the ventral nerve cord. The hyperplasia of the central nervous system seen in homozygous embryos is accompanied by loss of epidermal structures, including extensive loss of ventral and procephalic cuticle. The increase in the number of neurons is preceeded by an increase in the number of neuroblasts. ed^2B8/ed^ts animals shifted to the non-permissive temperature (29^oC) for 3 days at the end of larval stage 2 and then shifted back to 18^oC can survive to adulthood. These flies have extra macrochaetae, which are generally found next to macrochaetae that are normally present in wild-type flies. The density of microchaetae is also increased and the flies have a rough eye phenotype. The adult escapers show irregular thickening of wing vein 2 and/or slight notching of the distal wing margin.

(Ahmed et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

ed^ts/ed^2B8 is a suppressor of abnormal neuroanatomy phenotype of N^int.G.UAS, Scer\GAL4^Kr.PM

(Ahmed et al., 2003)

Phenotype Manifest In

Enhancer of

Statement

Reference

ed[+]/ed^2B8 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, fred^RNAi.UAS

(Chandra et al., 2003)

Suppressor of

Statement

Reference

ed^ts/ed^2B8 is a suppressor of larval neuron phenotype of N^int.G.UAS, Scer\GAL4^Kr.PM

(Ahmed et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of neurons seen in embryos expressing N^{int.G.Scer\UAS} under the control of Scer\GAL4^Kr.PM is suppressed by ed^2B8/ed^ts at 29^oC.

(Ahmed et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ed^k01102

(Ahmed et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Excision of the P{lacW} insertion can completely revert the mutant phenotype.

(Ahmed et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ed^2B8

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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