FlyBase Allele Report: Dmel\Vang[UAS.cWa]

General Information

Symbol

Dmel\Vang^UAS.cWa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Wolff

FlyBase ID

FBal0152143

Feature type

allele

Associated gene

Dmel\Vang

Associated Insertion(s)

Carried in Construct

P{UAS-stbm.W}

Also Known As

UAS-Stbm

Key Links

Transgenic product class

wild_type

(Jenny et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Jenny et al., 2003)

Mutagen

in vitro construct

(Jenny et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-stbm.W}

Cytology

Description

UAS sequences drive expression of Vang.

(Jenny et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Vang

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ommatidium, with Scer\GAL4^hs.2sev

(Klein et al., 2006, Jenny et al., 2003)

trichome & adult abdomen, with Scer\GAL4^αTub84B.PL

(Lawrence et al., 2004)

trichome & adult abdomen | somatic clone, with Scer\GAL4^αTub84B.PL

(Lawrence et al., 2004)

trichome & adult abdomen | somatic clone | cell non-autonomous, with Scer\GAL4^αTub84B.PL

(Lawrence et al., 2004)

wing hair, with Scer\GAL4^dpp.blk1

(Jenny et al., 2005)

wing hair, with Scer\GAL4^nub.PU

(Chin and Mlodzik, 2013)

Detailed Description

Statement

Reference

Expression of Vang^Scer\UAS.cWa under the control of Scer\GAL4^nub.PU results in a minor planar polarity phenotype in the wing (patches of misoriented hairs).

(Chin and Mlodzik, 2013)

Expression of Vang^Scer\UAS.cWa under the control of Scer\GAL4^dpp.blk1 only very rarely leads to cell orientation defects in the wing.

(Courbard et al., 2009)

Expression of Vang^Scer\UAS.cWa in the eye, under the control of Scer\GAL4^hs.2sev, causes mild planar polarity defects.

(Klein et al., 2006)

Expression of Vang^Scer\UAS.cWa under the control of Scer\GAL4^dpp.blk1 in a stripe along the proximo-distal axis of the wing results in a reorientation of wing hairs, which now point towards from the expression domain.

(Jenny et al., 2005)

Vang^Scer\UAS.cWa; Scer\GAL4^αTub84B.PL flies are viable and have largely normal planar polarity.

In the abdomens of Vang^Scer\UAS.cWa; Scer\GAL80^αTub84B.PL; Scer\GAL4^αTub84B.PL flies, somatic clones lacking Scer\GAL80^αTub84B.PL affect planar polarity in two ways: (i) hairs behind the clone are usually reversed, but (ii) hairs inside and mainly at the back of the clone are disheveled. The non-autonomous affect of these clones on tissue polarity can act across the anterior-posterior compartment boundary.

(Lawrence et al., 2004)

When Vang^Scer\UAS.cWa is driven by Scer\GAL4^hs.2sev at 29^oC, about 2% of ommatidia exhibit planar polarity defects.

(Jenny et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, Vang^UAS.cWa has abnormal planar polarity phenotype, enhanceable by fw[+]/fw⁴²

(Chin and Mlodzik, 2013)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, enhanceable by dco^j3B9/dco[+]

(Klein et al., 2006)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, enhanceable by dgo³⁸⁰

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, enhanceable by pk^pk-sple-13

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, enhanceable by pk^pk-sple-26

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, enhanceable by stan^E45

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, enhanceable by stan^E59

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, enhanceable by dgo³⁰⁸

(Jenny et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, non-enhanceable by N^55e11

(Jenny et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PL, Vang^UAS.cWa has abnormal planar polarity | somatic clone | cell non-autonomous phenotype, suppressible by stan^E59/stan^E59

(Lawrence et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, Vang^UAS.cWa has abnormal cell polarity phenotype, non-suppressible by N^55e11

(Jenny et al., 2003)

Enhancer of

Statement

Reference

Scer\GAL4^hs.2sev/Vang^UAS.cWa is an enhancer of abnormal planar polarity phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, Vang^UAS.cWa, scrib^UASp.GFP has abnormal planar polarity phenotype

(Courbard et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^nub.PU, Vang^UAS.cWa has wing hair phenotype, enhanceable by fw[+]/fw⁴²

(Chin and Mlodzik, 2013)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, enhanceable by dco^j3B9/dco[+]

(Klein et al., 2006)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, enhanceable by dgo³⁸⁰

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, enhanceable by pk^pk-sple-13

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, enhanceable by pk^pk-sple-26

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, enhanceable by stan^E45

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, enhanceable by stan^E59

(Jenny et al., 2003)

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, enhanceable by dgo³⁰⁸

(Jenny et al., 2003)

NOT Enhanced by

Statement

Reference

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, non-enhanceable by N^55e11

(Jenny et al., 2003)

Suppressed by

Statement

Reference

Scer\GAL4^αTub84B.PL, Vang^UAS.cWa has trichome & adult abdomen | somatic clone | cell non-autonomous phenotype, suppressible by stan^E59/stan^E59

(Lawrence et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^hs.2sev, Vang^UAS.cWa has ommatidium phenotype, non-suppressible by N^55e11

(Jenny et al., 2003)

Enhancer of

Statement

Reference

Scer\GAL4^hs.2sev/Vang^UAS.cWa is an enhancer of ommatidium phenotype of Scer\GAL4^hs.2sev, nmo^UAS.cUa

(Mirkovic et al., 2011)

Other

Statement

Reference

Scer\GAL4^dpp.blk1, Vang^UAS.cWa, scrib^UASp.GFP has wing hair phenotype

(Courbard et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

fw⁴²/+ enhances the wing planar polarity phenotype caused by expression of Vang^Scer\UAS.cWa under the control of Scer\GAL4^nub.PU.

(Chin and Mlodzik, 2013)

Co-expression of Vang^Scer\UAS.cWa enhances the ommatidial over-rotation phenotype which is caused by expression of nmo^Scer\UAS.cUa under the control of Scer\GAL4^hs.2sev.

(Mirkovic et al., 2011)

Co-expression of scrib^{Scer\UAS.P\T.T:Avic\GFP} and Vang^Scer\UAS.cWa under the control of Scer\GAL4^dpp.blk1 in the wing results in robust reorientation of cells outside the expression domain towards the expression domain.

(Courbard et al., 2009)

The planar polarity defects seen in the eye when Vang^Scer\UAS.cWa is expressed under the control of Scer\GAL4^hs.2sev in a wild-type background are significantly enhanced in a dco^j3B9/+ background.

(Klein et al., 2006)

The non-autonomous effect on planar polarity of Vang^Scer\UAS.cWa; Scer\GAL4^αTub84B.PL somatic clones (produced by the Scer\GAL80 MARCM method) in the adult abdomen is suppressed by pwn/pwn stan^E59/stan^E59. Cells within the resulting clones have randomised planar polarity.

(Lawrence et al., 2004)

The addition of pk^pk-sple-13 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 20% of ommatidia exhibit show defects. The addition of pk^pk-sple-26 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 14% of ommatidia exhibit show defects. The addition of dgo³⁰⁸ to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals partially enhances the planar polarity defects - about 5% of ommatidia exhibit show defects. The addition of dgo³⁸⁰ to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 12% of ommatidia exhibit show defects. The addition of stan^E45 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 25% of ommatidia exhibit show defects. The addition of stan^E59 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals enhances the planar polarity defects - about 25% of ommatidia exhibit show defects. The addition of N^55e11 to Vang^Scer\UAS.cWa, Scer\GAL4^hs.2sev animals has no effect on the planar polarity defects.

(Jenny et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Vang^Scer\UAS.cWa

Vang^UAS.cWa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Wolff

(Jenny et al., 2003)

Secondary FlyBase IDs

References (9)

Report Sections

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