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General Information
Symbol
Dmel\VangUAS.cWa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Wolff
FlyBase ID
FBal0152143
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-Stbm
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS sequences drive expression of Vang.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

trichome & adult abdomen, with Scer\GAL4αTub84B.PL

trichome & adult abdomen | somatic clone, with Scer\GAL4αTub84B.PL

trichome & adult abdomen | somatic clone | cell non-autonomous, with Scer\GAL4αTub84B.PL

Detailed Description
Statement
Reference

Expression of VangScer\UAS.cWa under the control of Scer\GAL4nub.PU results in a minor planar polarity phenotype in the wing (patches of misoriented hairs).

Expression of VangScer\UAS.cWa under the control of Scer\GAL4dpp.blk1 only very rarely leads to cell orientation defects in the wing.

Expression of VangScer\UAS.cWa in the eye, under the control of Scer\GAL4hs.2sev, causes mild planar polarity defects.

Expression of VangScer\UAS.cWa under the control of Scer\GAL4dpp.blk1 in a stripe along the proximo-distal axis of the wing results in a reorientation of wing hairs, which now point towards from the expression domain.

VangScer\UAS.cWa; Scer\GAL4αTub84B.PL flies are viable and have largely normal planar polarity.

In the abdomens of VangScer\UAS.cWa; Scer\GAL80αTub84B.PL; Scer\GAL4αTub84B.PL flies, somatic clones lacking Scer\GAL80αTub84B.PL affect planar polarity in two ways: (i) hairs behind the clone are usually reversed, but (ii) hairs inside and mainly at the back of the clone are disheveled. The non-autonomous affect of these clones on tissue polarity can act across the anterior-posterior compartment boundary.

When VangScer\UAS.cWa is driven by Scer\GAL4hs.2sev at 29oC, about 2% of ommatidia exhibit planar polarity defects.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

Scer\GAL4αTub84B.PL, VangUAS.cWa has trichome & adult abdomen | somatic clone | cell non-autonomous phenotype, suppressible by stanE59/stanE59

NOT suppressed by
Statement
Reference

Scer\GAL4hs.2sev, VangUAS.cWa has ommatidium phenotype, non-suppressible by N55e11

Enhancer of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

fw42/+ enhances the wing planar polarity phenotype caused by expression of VangScer\UAS.cWa under the control of Scer\GAL4nub.PU.

Co-expression of VangScer\UAS.cWa enhances the ommatidial over-rotation phenotype which is caused by expression of nmoScer\UAS.cUa under the control of Scer\GAL4hs.2sev.

Co-expression of scribScer\UAS.P\T.T:Avic\GFP and VangScer\UAS.cWa under the control of Scer\GAL4dpp.blk1 in the wing results in robust reorientation of cells outside the expression domain towards the expression domain.

The planar polarity defects seen in the eye when VangScer\UAS.cWa is expressed under the control of Scer\GAL4hs.2sev in a wild-type background are significantly enhanced in a dcoj3B9/+ background.

The non-autonomous effect on planar polarity of VangScer\UAS.cWa; Scer\GAL4αTub84B.PL somatic clones (produced by the Scer\GAL80 MARCM method) in the adult abdomen is suppressed by pwn/pwn stanE59/stanE59. Cells within the resulting clones have randomised planar polarity.

The addition of pkpk-sple-13 to VangScer\UAS.cWa, Scer\GAL4hs.2sev animals enhances the planar polarity defects - about 20% of ommatidia exhibit show defects. The addition of pkpk-sple-26 to VangScer\UAS.cWa, Scer\GAL4hs.2sev animals enhances the planar polarity defects - about 14% of ommatidia exhibit show defects. The addition of dgo308 to VangScer\UAS.cWa, Scer\GAL4hs.2sev animals partially enhances the planar polarity defects - about 5% of ommatidia exhibit show defects. The addition of dgo380 to VangScer\UAS.cWa, Scer\GAL4hs.2sev animals enhances the planar polarity defects - about 12% of ommatidia exhibit show defects. The addition of stanE45 to VangScer\UAS.cWa, Scer\GAL4hs.2sev animals enhances the planar polarity defects - about 25% of ommatidia exhibit show defects. The addition of stanE59 to VangScer\UAS.cWa, Scer\GAL4hs.2sev animals enhances the planar polarity defects - about 25% of ommatidia exhibit show defects. The addition of N55e11 to VangScer\UAS.cWa, Scer\GAL4hs.2sev animals has no effect on the planar polarity defects.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
VangScer\UAS.cWa
VangUAS.cWa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Wolff
Secondary FlyBase IDs
    References (9)