FlyBase Allele Report: Dmel\rno[3]

General Information

Symbol

Dmel\rno³

Species

D. melanogaster

Name

FlyBase ID

FBal0154694

Feature type

allele

Associated gene

Dmel\rno

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Voas and Rebay, 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q617term.

(Voas and Rebay, 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:196,423..196,423 [-]

Nucleotide change:

C196423T

Amino acid change:

Q618term | rno-PB; Q618term | rno-PC; Q618term | rno-PD

Reported amino acid change:

Q617term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Voas and Rebay, 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell number | pupal stage | somatic clone

(Steele et al., 2009)

increased cell number | somatic clone | third instar larval stage

(Steele et al., 2009)

lethal (with Df(3L)XKR845)

(Voas and Rebay, 2003)

lethal (with Df(3L)XZB970)

(Voas and Rebay, 2003)

lethal | recessive

(Voas and Rebay, 2003)

Phenotype Manifest In

cone cell | pupal stage | somatic clone

(Steele et al., 2009)

ommatidium | pupal stage | somatic clone

(Steele et al., 2009)

photoreceptor cell R8 | somatic clone | third instar larval stage

(Steele et al., 2009)

Detailed Description

Statement

Reference

rno³ somatic clones generated during eye development result in mostly normal adult eye morphology.

Occasionally (in less than 5% of cases) rno³ third instar larval eye disc clones develop multiple photoreceptor R8 cells within a single ommatidial cluster.

48 hours after puparium formation, rno³ mutant eye disc ommatidia contain five cone cells per cluster, increased from four per cluster in wildtype ommatidia.

rno³ mutant clones do not show increased apoptosis or cell proliferation in the developing eye disc.

(Steele et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

rno³ has increased cell number | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

rno³ has increased cell number | pupal stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

NOT Enhanced by

Statement

Reference

Rbf^15aΔ, rno³ has increased cell number | third instar larval stage | somatic clone phenotype, non-enhanceable by Dronc¹

(Steele et al., 2009)

rno³ has increased cell number | pupal stage | somatic clone phenotype, non-enhanceable by E2f1^su89

(Steele et al., 2009)

Suppressed by

Statement

Reference

Rbf^15aΔ, rno³ has increased cell death | somatic clone phenotype, suppressible by Dronc¹

(Steele et al., 2009)

NOT Enhancer of

Statement

Reference

rno³ is a non-enhancer of increased cell death | somatic clone phenotype of Rbf^15aΔ

(Steele et al., 2009)

rno³ is a non-enhancer of increased cell number | third instar larval stage | somatic clone phenotype of Rbf^15aΔ

(Steele et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

rno³ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

rno³ has cone cell | pupal stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

rno³ has ommatidium | pupal stage | somatic clone phenotype, enhanceable by Rbf^15aΔ

(Steele et al., 2009)

NOT Enhanced by

Statement

Reference

Rbf^15aΔ, rno³ has eye | somatic clone phenotype, non-enhanceable by Dronc¹

(Steele et al., 2009)

Rbf^15aΔ, rno³ has interommatidial bristle | somatic clone phenotype, non-enhanceable by Dronc¹

(Steele et al., 2009)

Rbf^15aΔ, rno³ has photoreceptor cell R8 | third instar larval stage | somatic clone phenotype, non-enhanceable by Dronc¹

(Steele et al., 2009)

Suppressed by

Statement

Reference

Rbf^15aΔ, rno³ has eye disc | somatic clone phenotype, suppressible by Dronc¹

(Steele et al., 2009)

Rbf^15aΔ, rno³ has photoreceptor cell R8 | somatic clone | third instar larval stage phenotype, suppressible by Delta^UAS.cHa/Scer\GAL4^h-H10

(Steele et al., 2009)

Enhancer of

Statement

Reference

rno³/rno[+] is an enhancer of eye phenotype of aos^Δ7/aos⁰⁵⁸⁴⁵

(Voas and Rebay, 2003)

NOT Enhancer of

Statement

Reference

rno³ is a non-enhancer of eye disc | somatic clone phenotype of Rbf^15aΔ

(Steele et al., 2009)

rno³ is a non-enhancer of eye disc posterior to the morphogenetic furrow | third instar larval stage | somatic clone phenotype of Rbf^15aΔ

(Steele et al., 2009)

Other

Statement

Reference

Rbf^15aΔ, rno³ has eye | somatic clone phenotype

(Steele et al., 2009)

Rbf^15aΔ, rno³ has interommatidial bristle | somatic clone phenotype

(Steele et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Doubly mutant rno³ and Rbf^15aΔ somatic clones show shiny-eye differentiation defects. Pupal eye discs are largely devoid of bristle cells, and adult eyes lack bristles. Roughly 20% of third instar larval eye disc clones show multiple photoreceptor R8 cells in some ommatidial clusters. This phenotype is not enhanced in a Nc¹ background.

48 hours after puparium formation Rbf^15aΔ; rno³ mutant eye disc ommatidia contain a variable number of cone cells per cluster. Some contain almost twice the number of normal cone cells, and ommatidial arrangement is very disorganised compared to control tissue.

Expression of Dl^Scer\UAS.cHa under the control of Scer\GAL4^h-H10 significantly suppresses the multiple photoreceptor-R8 phenotype in rno³; Rbf^15aΔ somatic clones in the eye.

The significant amounts of apoptosis observed near the morphogenetic furrow in Rbf^15aΔ mutant clones is not enhanced by rno³. However, Rbf^15aΔ; rno³ mutant clones carrying an additional mutation in Nc¹ show significantly decreased levels of apoptosis.

Rbf^15aΔ; rno³ mutant clones show increased cell proliferation in the posterior region of the wing disc, similar to discs mutant only for Rbf^15aΔ.

(Steele et al., 2009)

The eye blistering phenotype seen in argos^Δ7/argos⁰⁵⁸⁴⁵ escaper adults is enhanced by rno³/+, so that it covers much of the eye.

(Voas and Rebay, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

rno^K

(Steele et al., 2009)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rno³

(Steele et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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