FlyBase Allele Report: Dmel\pita[1]

General Information

Symbol

Dmel\pita¹

Species

D. melanogaster

Name

FlyBase ID

FBal0154756

Feature type

allele

Associated gene

Dmel\pita

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Laundrie et al., 2003)

Progenitor genotype

Cytology

Description

Amino acid replacement: H472Y.

(Laundrie et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,549,729..23,549,729 [-]

Nucleotide change:

C23549729T

Amino acid change:

H472Y | pita-PA; H412Y | pita-PB

Reported amino acid change:

H472Y

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Laundrie et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage | recessive

(Laundrie et al., 2003)

melanotic mass phenotype | larval stage | recessive

(Laundrie et al., 2003)

some die during larval stage | recessive

(Laundrie et al., 2003)

Phenotype Manifest In

egg chamber | germline clone

(Laundrie et al., 2003)

nurse cell & nucleus | germ-line clone

(Laundrie et al., 2003)

nurse cell & polytene chromosome | germ-line clone

(Laundrie et al., 2003)

nurse cell | germline clone

(Laundrie et al., 2003)

Detailed Description

Statement

Reference

pita¹ homozygotes die as larvae with melanotic 'tumors'. Imaginal disc development appears normal in these animals. At stage 10 the nurse cell nuclei of pita¹ germ-line clone egg chambers show crater-like structures. At early stages some of these egg chambers lack nurse cell nuclei completely (25% n=420). This correlates with ectopic expression of cell-death markers in nurse cells. At subsequent stages the dumping of the nurse cell cytoplasm into the egg fails in 80% of egg chambers (n=460). Nurse cell polytene chromosomes fail to disperse after stage 5 (as in wild-type), but instead remain visible as discrete individualized blobs throughout late stages.

(Laundrie et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

pita⁰¹⁸⁶²

(Laundrie et al., 2003)

pita⁰⁸⁸⁵⁹

(Laundrie et al., 2003)

pita^k05606

(Laundrie et al., 2003)

pita⁰²¹³²

(Laundrie et al., 2003)

Rescued by

pita¹ is rescued by pita^hs.PL

(Laundrie et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

pita1

(McCall, 2014.7.9)

pita¹

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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