FlyBase Allele Report: Dmel\Syn[97]

P-element mutagenesis using the Syn^P1+P2 strain containing the P{lacW}Syn^P1 and P{lacW}Syn^P2 insertions has resulted in precise excision of the P{lacW}Syn^P2 insertion together with an imprecise excision of the P{lacW}Syn^P1 insertion. The imprecise excision results in the deletion of 1397bp around the original insertion site. The deletion eliminates 349bp of the presumed promoter region including the putative transcription start site, the first known exon (207bp) and 841bp of the first intron, while the coding region remains unaffected.

(Godenschwege et al., 2004)

A precise excision of the P{lacW}Syn^P2 insertion present in the Syn^P1+P2 mutant, together with a 1.5kb deletion around the P{lacW}Syn^P1 insertion present in the Syn^P1+P2 mutant, eliminating the promoter and the first exon of the Syn gene.

(Godenschwege et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

3R:10,190,339..10,191,735 [+]

Comment:

Reported as a 1397bp deletion, which removes 349bp of untranstlated sequences, the 207bp first exon, and 841bp of the first intron.

(Godenschwege et al., 2004, Godenschwege et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Noonan syndrome with multiple lentigines 1

modeled by Hsap\PTPN11^Q510P.UAS

(Leahy et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

neuromuscular junction

(Vasin et al., 2014)

NMJ bouton

(Vasin et al., 2014)

synaptic vesicle

(Vasin et al., 2014)

Detailed Description

Statement

Reference

Syn⁹⁷ mutant third instar larvae do not display any abnormalities in the pattern or duration of peristaltic wave contractions and adult survival rate is comparable to controls.

(Shilts and Broadie, 2017)

Syn⁹⁷/+ and Syn⁹⁷/Syn⁹⁷ mutant flies exhibit significantly decreased sedation sensitivity to acute ethanol exposure in naive flies, and a significant decrease in sedation tolerance when repeatedly exposed to ethanol, as compared to controls.

(Engel et al., 2016)

Syn⁹⁷ mutants exhibit a significant reduction in the number of 'ghost' boutons (lacking postsynaptic specializations in resting and high-K[+]-stimulated preparations).

K[+]-stimulated growth in Syn⁹⁷ larvae is reduced by approximately 60%.

Syn⁹⁷ mutants do not exhibit defects in the number of synaptic boutons per neuromuscular junction in the 3rd instar larval stage.

Synaptic vesicle depletion in Syn⁹⁷ synapses may be the result of their impaired growth and development.

In wild-type flies, forskolin pretreatment of neuromuscular junctions significantly promotes activity-dependent synaptic growth. This effect is completely abolished in Syn⁹⁷ mutants.

(Vasin et al., 2014)

Syn⁹⁷ homozygote adult flies are seizure-prone (their seizure behavior includes hyperactivity resulting in flies flipping on their backs) and show significantly longer recovery times in a modified bang-sensitivity assay.

(Paemka et al., 2013)

Syn⁹⁷ larvae show a 50% reduction in an odor-sugar associative learning paradigm, while are still able to taste, smell and move. Susceptibility to stress and olfactory adaptation are unchanged.

(Michels et al., 2011)

Mutants show no obvious anatomical defects in the synaptic neuropil of the adult brain.

The number and ultrastructure of synaptic boutons at the neuroumuscular junction of muscles 12/13 and 6/7 is not significantly different in mutant larvae compared to wild type.

The mean excitatory junction potential (EJP) amplitude at 0.5Hz stimulus frequency and the spontaneous miniature EJP amplitude and frequency at the mutant larval neuromuscular junction are normal.

Wing beat frequency during tethered flight is significantly increased in mutant flies compared to controls. Mutant flies show increased walking activity compared to controls, with a significant difference in the early phase (60-120 minutes) but not in the late phase of activity.

Optomotor responses of mutant flies are normal at low pattern speeds. However, fast moving patterns are followed significantly more efficiently by the mutant flies than by wild-type flies.

Mutant flies habituate significantly faster than mutant flies in an olfactory jump assay in response to repeated benzaldehyde odour exposure.

Mutant flies show a significantly higher ethanol tolerance than wild-type flies in an inebriometer assay.

Mutant flies are slightly but significantly impaired in heat avoidance during training in the heat-box paradigm for place learning.

Mutant flies perform significantly poorer than wild-type in a 3-minute memory test in an olfactory associative learning assay.

Mutant male flies without mating experience court mated females significantly less persistently than do wild-type males during a 20-minute period. Like wild-type males, the mutant males stop courting males at the end of this period, demonstrating their ability to learn. However, after 30 and 180 minutes in isolation, the mutant males are significantly more likely than wild-type males to resume courting mated females during a 5-minute exposure, indicating a defect in memory.

(Godenschwege et al., 2004)

Syn⁹⁷ mutants appear wild-type in terms of adult morphology and vitality.

Longevity is weakly reduced in Syn⁹⁷ mutants.

(Godenschwege et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Syn⁹⁷ is rescued by Scer\GAL4^elav-C155/Syn^UAS.cVa

(Vasin et al., 2014)

Syn⁹⁷ is rescued by Scer\GAL4^elav-C155/Syn^1-12.UAS

(Michels et al., 2011)

Syn⁹⁷ is rescued by Scer\GAL4^elav-C155/Syn^1-12.UAS/Scer\GAL80^ts.αTub84B

(Michels et al., 2011)

Syn⁹⁷ is rescued by Scer\GAL4^Mef2.247/Syn^1-12.UAS

(Michels et al., 2011)

Not rescued by

Syn⁹⁷ is not rescued by Syn^1-12.UAS/Scer\GAL4^GH146

(Michels et al., 2011)

Syn⁹⁷ is not rescued by Syn^1-12.UAS/Scer\GAL4^NP0225

(Michels et al., 2011)

Syn⁹⁷ is not rescued by Scer\GAL4^elav-C155/Syn^PKA-AA.UAS

(Michels et al., 2011)

Comments

The addition of Syn^Scer\UAS.cVa (under the control of Scer\GAL4^elav-C155) to a Syn⁹⁷ mutant background rescues the 'ghost' bouton phenotype, but does not promote futher (over)growth.

(Vasin et al., 2014)

Pan-neuronal expression of Syn^{1-12.Scer\UAS}, under the control of Scer\GAL4^elav-C155 fully rescues associative learning in Syn⁹⁷ mutants.

Pan-neuronal acute expression of Syn^{1-12.Scer\UAS}, through heat-shocking of flies carrying Scer\GAL80^ts.αTub84B as well as Scer\GAL4^elav-C155 (where Scer\GAL80^ts.αTub84B blocks expression until heat-shock), fully rescues the associative odor-sugar learning in Syn⁹⁷ mutants.

Expression of Syn^{1-12.Scer\UAS} in the mushroom bodies, under the control of Scer\GAL80^Mef2.PT, fully rescues the associative odor-sugar learning in Syn⁹⁷ mutants.

Expression of Syn^{1-12.Scer\UAS} in the projection neurons, under the control of either Scer\GAL4^GH146 or Scer\GAL4^NP0225, fails to rescue the associative odor-sugar learning in Syn⁹⁷ mutants.

Pan-neuronal expression of Syn^{1-12.Scer\UAS}, under the control of Scer\GAL4^elav-C155 fails to rescue the associative learning defects found in Syn⁹⁷ mutants.

(Michels et al., 2011)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

83706

Syn⁹⁷

29031

w^* P{GawB}MzVum; Syn⁹⁷

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Df(3)Synapsin⁹⁷

(Sanchez-Soriano et al., 2005)

Syn⁹⁷

(Blanco-Redondo et al., 2019, Ojelade et al., 2019, Robinson et al., 2018, Engel et al., 2016, Vasin et al., 2014, Paemka et al., 2013, Michels et al., 2011, Nuwal et al., 2011, Godenschwege et al., 2004, Godenschwege et al., 2000)

Syn^97CS

(Michels et al., 2011)

syn97

(Gerber et al., 2003)

syn⁹⁷

(Leahy et al., 2024, Shilts and Broadie, 2017, Busch et al., 2009)

syn^97CS

(Niewalda et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (18)

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