FlyBase Allele Report: Dmel\InR[K414P.UAS]

General Information

Symbol

Dmel\InR^K414P.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0156357

Feature type

allele

Associated gene

Dmel\InR

Associated Insertion(s)

Carried in Construct

P{UAS-InR.K414P}

Also Known As

UAS-InR^R418P, UAS-InR^CA

Key Links

Genomic Maps

JBrowse

Transgenic product class

missense_variant

(Parks, 2020.2.14)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Parks, 2020.2.14)

Mutagen

in vitro construct

(Parks, 2020.2.14)

Progenitor genotype

Carried in construct

P{UAS-InR.K414P}

Cytology

Description

UAS regulatory sequences drive expression of InR that carries an K418P amino acid replacement (numbering using GenBank submission gb|AAC47458). This change corresponds to K414P in the current release of the Drosophila genome (release 6). The change is meant to mimic the R113P change in Longo et al., 1993 (PMID:8419945) (R113P is called R86P in the paper). (Revision of data in FBrf0178856, where it was incorrectly annotated as carrying a R418P change).

(Parks, 2020.2.14)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

InR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:21,579,144..21,579,145 [-]

Nucleotide change:

AA21579145CC

Amino acid change:

K414P | InR-PA; K414P | InR-PB; K414P | InR-PC; K414P | InR-PD

Reported amino acid change:

K414P

Comment:

Analogous R113P mutation in human INSR implicated in Donohue syndrome; mutation carried on in vitro construct; site of nucleotide substitution in fly gene inferred by FlyBase curator based on reported amino acid change.

(Parks, 2020.2.14)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates tauopathy

modeled by Hsap\MAPT^UAS.cWa

(Pragati and Sarkar, 2025, Pragati and Sarkar, 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Parks, 2020.2.14)

INSR:p.Arg113Pro

(FlyBase curators, 2019-)

Variants Synonym(s)

INSR:p.Arg86Pro

Associated human disease model(s)

External database links

ClinVar: INSR_14703

Comments concerning this variant

Phenotypic Data

Phenotypic Class

abnormal sleep | adult stage, with Scer\GAL4^Dsk.PP

(Palermo et al., 2022)

increased cell number | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

increased rate of feeding behavior | adult stage, with Scer\GAL4^Dsk.PP

(Palermo et al., 2022)

lethal, with Scer\GAL4^hs.PB

(Lee et al., 2024)

Phenotype Manifest In

abdominal dorsal adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

abdominal lateral adult muscle precursor cell | third instar larval stage, with Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Detailed Description

Statement

Reference

Expression of InR^{R418P.Scer\UAS} under the control of Scer\GAL4^{E(spl)m6-BFM.PF} leads to significantly increased numbers of abdominal lateral and dorsal, but not ventral, adult muscle precursor cells in third instar larvae, as compared to controls; this expression does not affect the number of mitotic adult muscle precursor cells at embryonic stages, as compared to controls.

(Aradhya et al., 2015)

Fasted third instar larvae expressing InR^{R418P.Scer\UAS} under the control of Scer\GAL4^NPFR1.6.6 display an attenuated feeding response to solid food. These third instar larvae do not exhibit significant changes in the intake rate of the richer liquid food, relative to controls.

(Wu et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF} has increased cell number | third instar larval stage phenotype, suppressible by N^KK102890, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/InR^K414P.UAS is a suppressor of decreased cell number | oogenesis phenotype of BubR1^K1204A

(Tang et al., 2020)

NOT Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/InR^K414P.UAS is a non-suppressor of decreased cell number | adult stage | progressive phenotype of Uch^KO

(Lee et al., 2024)

Other

Statement

Reference

InR^K414P.UAS, Myc^KK103869, Scer\GAL4^{E(spl)m6-BFM.PF} has decreased cell number | third instar larval stage phenotype

(Aradhya et al., 2015)

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}, krz^UAS.cRa has increased cell number | third instar larval stage phenotype

(Aradhya et al., 2015)

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF}, dx^UAS.cMa has increased cell number | third instar larval stage phenotype

(Aradhya et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF} has abdominal lateral adult muscle precursor cell | third instar larval stage phenotype, suppressible by N^KK102890, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

InR^K414P.UAS, Scer\GAL4^{E(spl)m6-BFM.PF} has abdominal dorsal adult muscle precursor cell | third instar larval stage phenotype, suppressible by N^KK102890, Scer\GAL4^{E(spl)m6-BFM.PF}

(Aradhya et al., 2015)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Tub.PU/InR^K414P.UAS is a non-enhancer of intestinal stem cell | adult stage | decreased number phenotype of BubR1^K1204A

(Tang et al., 2020)

Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/InR^K414P.UAS is a suppressor of mechanosensory neuron of leg campaniform sensillum | decreased number phenotype of Uch^KO

(Lee et al., 2024)

Scer\GAL4^Tub.PU/InR^K414P.UAS is a suppressor of germarium cap cell | decreased number phenotype of BubR1^K1204A

(Tang et al., 2020)

Scer\GAL4^Tub.PU/InR^K414P.UAS is a suppressor of adult enteroendocrine cell | decreased number phenotype of BubR1^K1204A

(Tang et al., 2020)

NOT Suppressor of

Statement

Reference

Scer\GAL4^VGlut1-OK371/InR^K414P.UAS is a non-suppressor of mechanosensory neuron of leg campaniform sensillum | decreased number phenotype of Uch^KO

(Lee et al., 2024)

Scer\GAL4^Tub.PU/InR^K414P.UAS is a non-suppressor of intestinal stem cell | adult stage | decreased number phenotype of BubR1^K1204A

(Tang et al., 2020)

Other

Additional Comments

Genetic Interactions

Statement

Reference

The increased number of abdominal lateral and dorsal adult muscle precursor cells present in third instar larvae expressing InR^{R418P.Scer\UAS} under the control of Scer\GAL4^{E(spl)m6-BFM.PF} is suppressed by co-expressing N^KK102890, but no significant effect on the number of abdominal ventral adult muscle precursor cells is seen in these larvae, as compared to controls.

Co-expression of Myc^KK103869 suppresses the increased number of abdominal lateral and dorsal adult muscle precursor cells present in third instar larvae expressing InR^{R418P.Scer\UAS} under the control of Scer\GAL4^{E(spl)m6-BFM.PF}, even leading to their decrease in numbers, as compared to controls; co-expressing Myc^KK103869 and InR^{R418P.Scer\UAS} also results in decreased numbers of abdominal ventral adult muscle precursor cells in third instar larvae, as compared to controls.

Expression of either dx^Scer\UAS.cMa or krz^Scer\UAS.cRa in combination with InR^{R418P.Scer\UAS} under the control of Scer\GAL4^{E(spl)m6-BFM.PF} results in increased numbers of abdominal lateral adult muscle precursor cells present in third instar larvae, as compared to controls.

(Aradhya et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

8250

y¹ w¹¹¹⁸; P{UAS-InR.K414P}2

8528

P{UAS-InR.K414P}1, y¹ w¹¹¹⁸

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

InR^K414P.UAS

InR^{R418P.Scer\UAS}

InR^R418P.UAS

InR^R418P

(Hertenstein et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (17)

Report Sections

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