FlyBase Allele Report: Dmel\E(spl)m8-HLH[SD.UAS]

General Information

Symbol

Dmel\E(spl)m8-HLH^SD.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0156613

Feature type

allele

Associated gene

Dmel\E(spl)m8-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-m8.SD}

Also Known As

UAS-m8SD

Key Links

Transgenic product class

missense_variant

(Karandikar et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Karandikar et al., 2004)

Mutagen

in vitro construct

(Karandikar et al., 2004)

Progenitor genotype

E(spl)m8-HLH^UAS.cKa

(Karandikar et al., 2004)

Carried in construct

P{UAS-m8.SD}

Cytology

Description

Amino acid replacement: A159D.

(Karandikar et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)m8-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye, with Scer\GAL4^sca-109-68

(Kahali et al., 2010, Kahali et al., 2009, Karandikar et al., 2004)

eye, with Scer\GAL4^sca-537.4

(Kahali et al., 2009, Bose et al., 2006, Karandikar et al., 2004)

eye photoreceptor cell, with Scer\GAL4^sca-109-68

(Kahali et al., 2009)

interommatidial bristle, with Scer\GAL4^sca-537.4

(Kahali et al., 2009, Karandikar et al., 2004)

macrochaeta, with Scer\GAL4^sca-537.4

(Karandikar et al., 2004)

macrochaeta & scutellum, with Scer\GAL4^455.2

(Karandikar et al., 2004)

ommatidium, with Scer\GAL4^sca-109-68

(Kahali et al., 2010, Kahali et al., 2009, Karandikar et al., 2004)

ommatidium, with Scer\GAL4^sca-537.4

(Karandikar et al., 2004)

scutellar bristle, with Scer\GAL4^455.2

(Kahali et al., 2009)

scutellar bristle, with Scer\GAL4^sca-109-68

(Kahali et al., 2010)

scutellar bristle, with Scer\GAL4^sca-537.4

(Kahali et al., 2009)

Detailed Description

Statement

Reference

Expression of E(spl)^SD.Scer\UAS within the morphogenetic furrow of the developing eye under the control of Scer\GAL4^sca-109-68 results in a reduced eye with defects in ommatidial (facet) size and patterning.

Flies expressing E(spl)^SD.Scer\UAS under the control of Scer\GAL4^sca-109-68 display ~411 eye facets, compared to ~779 in wild-type controls.

Expression of E(spl)^SD.Scer\UAS within the morphogenetic furrow of the developing eye under the control of Scer\GAL4^sca-109-68 results in a strong loss of the four scutellar macrochaeta.

(Kahali et al., 2010)

Expression of E(spl)m8-HLH^SD.Scer\UAS with Scer\GAL4^sca-109-68 elicits a moderately reduced eye at 24[o]C, with varied ommatidial size. Photoreceptor clusters have reduced numbers of secondary R-cells.

Expression of E(spl)m8-HLH^SD.Scer\UAS with Scer\GAL4^sca-537.4 elicits a severely reduced eye and loss of the scutellar macrochaetes and interommatidial bristles.

Expression of E(spl)m8-HLH^SD.Scer\UAS with Scer\GAL4^455.2 results in potent loss of scutellar macrochaetes.

(Kahali et al., 2009)

Expression of E(spl)^SD.Scer\UAS under the control of Scer\GAL4^sca-537.4 leads to severely reduced eyes.

(Bose et al., 2006)

When E(spl)^Scer\UAS.cKa is driven by Scer\GAL4^455.2, the number of macrochaetae seen on the scutellum is reduced from 4 to zero. When E(spl)^SD.Scer\UAS is driven by Scer\GAL4^sca-537.4 a potent bristle suppression is seen. These mutants also exhibit a strong reduced eye phenotype. Ommatidial bristles are suppressed, and a severe attenuation of the number of ommatidia is seen, down to about 25 ommatidia per eye. Some of the ommatidia exhibit a dimpled, "blueberry" phenotype. In the eye disc a reduction of the numbers of "R8 founder" cells is seen. Pervasive and biphasic apoptosis is seen behind the morphogenetic furrow in the larval eye disc. When E(spl)^Scer\UAS.cKa is driven by Scer\GAL4^sca-109-68, a reduced eye phenotype is seen, with a roughening of the eye. When E(spl)^SD.Scer\UAS is driven by Scer\GAL4^h-1J3, Scer\GAL4^GMR.PF or Scer\GAL4^hs.2sev no eye phenotype is seen.

(Karandikar et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

E(spl)m8-HLH^SD.UAS, Scer\GAL4^455.2 has scutellar bristle phenotype, non-enhanceable by Df(3R)Espl22/+

(Kahali et al., 2009)

Suppressed by

Statement

Reference

E(spl)m8-HLH^SD.UAS, Scer\GAL4^sca-109-68 has eye phenotype, suppressible by ato^UAS.cJa, Scer\GAL4^sca-109-68

(Kahali et al., 2009)

E(spl)m8-HLH^SD.UAS, Scer\GAL4^sca-109-68 has eye photoreceptor cell phenotype, suppressible by ato^UAS.cJa, Scer\GAL4^sca-109-68

(Kahali et al., 2009)

NOT suppressed by

Statement

Reference

E(spl)m8-HLH^SD.UAS, Scer\GAL4^455.2 has scutellar bristle phenotype, non-suppressible by Df(3R)Espl22/+

(Kahali et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The eye and photoreceptor defects caused by expression of E(spl)m8-HLH^SD.Scer\UAS with Scer\GAL4^sca-109-68 are suppressed by co-expression of ato^Scer\UAS.cJa.

The potent loss of scutellar macrochaetes in E(spl)m8-HLH^SD.Scer\UAS Scer\GAL4^455.2 flies is not affected by Df(3R)Espl22/+.

(Kahali et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

E(spl)m8-HLH^SD.UAS is rescued by Scer\GAL4^sca-109-68/E(spl)m8-HLH^{123-179.CtD.UAS}

(Kahali et al., 2010)

Not rescued by

E(spl)m8-HLH^SD.UAS is not rescued by E(spl)m8-HLH^{S159D.123-179.CtD.UAS}/Scer\GAL4^sca-109-68

(Kahali et al., 2010)

Comments

Co-expression of E(spl)^{123-179.CtD.Scer\UAS} under the control of Scer\GAL4^sca-109-68 strongly rescues the reduced eye found upon expression of E(spl)^SD.Scer\UAS in the developing eye morphogenetic furrow (Scer\GAL4^sca-109-68) and significantly restores the aberrant ommatidial size and patterning.

Co-expression of E(spl)^{S159D.123-179.CtD.Scer\UAS} under the control of Scer\GAL4^sca-109-68 does not rescue the reduced eye found upon expression of E(spl)^SD.Scer\UAS nor the aberrant ommatidial size or patterning.

Co-expression of E(spl)^{123-179.CtD.Scer\UAS} elicits facet numbers (~650-670) that are significantly higher than the baseline in Scer\GAL4^sca-109-68 E(spl)^SD.Scer\UAS flies. The four scutellar macrochaeta lost in Scer\GAL4^sca-109-68 E(spl)^SD.Scer\UAS mutants are restored upon E(spl)^{123-179.CtD.Scer\UAS} co-expression.

Co-expression of E(spl)^{S159D.123-179.CtD.Scer\UAS} elicits facet numbers (~650-670) that are indistinguishable from those in Scer\GAL4^sca-109-68 E(spl)^SD.Scer\UAS flies.

(Kahali et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

E(spl)^SD.Scer\UAS

E(spl)m8-HLH^SD.Scer\UAS

E(spl)m8-HLH^SD.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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