FlyBase Allele Report: Dmel\Alk[act.UAS.Tag:HA]

General Information

Symbol

Dmel\Alk^{act.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0157355

Feature type

allele

Associated gene

Dmel\Alk

Associated Insertion(s)

Carried in Construct

P{UAS-Alk.act.HA}

Also Known As

UAS-Alk^CA

Key Links

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Englund et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-Alk.act.HA}

Cytology

Description

Expression of a constitutively active form of Alk, fused to the epitope tag Tag:HA, is under the control of UAS sequences.

(Englund et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Alk

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with Scer\GAL4^GS.spok

(Pan and O'Connor, 2021)

abnormal developmental rate, with Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

abnormal developmental rate, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

abnormal learning | adult stage, with Scer\GAL4^Alk.38, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^elav-C155, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal learning | adult stage, with Scer\GAL4^Ras64B.PW, Scer\GAL80^ts.αTub84B

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^386Y

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^Alk.38

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^elav-C155

(Gouzi et al., 2011)

abnormal size | pupal stage, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

decreased body size | P-stage, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

decreased cell size | adult stage, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

increased cell number | larval stage, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

increased cell number | larval stage, with Scer\GAL4^Hml.PG

(Zettervall et al., 2004)

increased size | larval stage, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

viable, with Scer\GAL4^elav-C155

(Gouzi et al., 2011)

Phenotype Manifest In

hemocyte, with Scer\GAL4^He.PZ

(Zettervall et al., 2004)

hemocyte, with Scer\GAL4^Hml.PG

(Zettervall et al., 2004)

prothoracic gland | larval stage, with Scer\GAL4^spok.1.45

(Pan and O'Connor, 2021)

wing, with Scer\GAL4^Ras64B.PW

(Gouzi et al., 2011)

Detailed Description

Statement

Reference

Pan-neuronal expression of Alk^{act.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of Alk^{act.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Ras64B.PW, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls. The wings of the resulting adult flies are also smaller than in wild type, due to reduced cell size rather than cell number.

Expression of Alk^{act.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Alk.38, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, causes significant deficits in associative olfactory learning compared to controls.

Expression of Alk^{act.Scer\UAS.T:Ivir\HA1} under the control of either Scer\GAL4^Mef2.247 or Scer\GAL4^c747, and restricted to the adult stages using Scer\GAL80^ts.αTub84B, does not affect associative olfactory learning.

Expression of Alk^{act.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^elav-C155, Scer\GAL4^Ras64B.PW, Scer\GAL4^Alk.38 or Scer\GAL4^386Y throughout development yields pupae that are 10-15% smaller than controls. The resulting adults are viable and normally patterned.

Expression of Alk^{act.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^Ilp2.PR throughout development yields pupae that are a similar size to controls.

(Gouzi et al., 2011)

When expression is driven by Scer\GAL4^He.PZ, hemocyte proliferation is increased though lamellocyte numbers and crystal cells are unaffected. Similar, though milder, effects are seen when expression is driven by Scer\GAL4^Hml.PG. When expression is driven by Scer\GAL4^Sgs4.PH, there is no effect on hemocytes.

(Zettervall et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Alk^{act.UAS.Tag:HA}, Scer\GAL4^phtm.PO has abnormal developmental rate phenotype, suppressible | partially by Stat92E^HMS00035, Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

Suppressor of