FlyBase Allele Report: Dmel\Ptth[120F2A]

General Information

Symbol

Dmel\Ptth^120F2A

Species

D. melanogaster

Name

FlyBase ID

FBal0345091

Feature type

allele

Associated gene

Dmel\Ptth

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

TALEN

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Shimell et al., 2018)

Mutagen

TALEN

(Shimell et al., 2018)

Progenitor genotype

Cytology

Description

7bp deletion (CGTTTGC, nucleotides 465-471 of the Ptth-RF coding sequence) in the final exon. Produces a frameshift distal to residue N154 of the Ptth-PR protein, with the protein continuing out of frame for 24 amino acid residues.

(Shimell et al., 2018)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:575,963..575,969 [-]

Comment:

A 7 bp deletion (CGTTTGC) in the final exon of Ptth causes a frameshift in codon 155 and early translation termination after the addition of 23 novel amino acids.

(Shimell et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate

(Pan and O'Connor, 2021)

abnormal developmental rate | larval stage

(Shimell et al., 2018)

abnormal developmental rate | larval stage (with Ptth^8K1J)

(Shimell et al., 2018)

abnormal developmental rate | larval stage (with Ptth^Delta)

(Shimell et al., 2018)

increased body size | pupal stage

(Shimell et al., 2018)

Phenotype Manifest In

Detailed Description

Statement

Reference

Ptth^120F2A and Ptth^120F2A/Ptth^8K1J mutants exhibit a 1-day delay in the developmental time from L1 to pupariation, as compared to controls; third instar larvae gain weight at the same rate as control larvae. The larval critical weight, critical period and terminal growth period are increased as compared to controls although the minimal viable weight occurs slightly earlier than the critical weight and at a lower weight.

Adult males are significantly heavier than controls.

(Shimell et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Ptth^120F2A, Pvr^GD14375, Scer\GAL4^phtm.PO has increased body size | P-stage phenotype, enhanceable by Alk^JF02668, Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

Alk^JF02668, Ptth^120F2A, Scer\GAL4^phtm.PO has increased mortality during development phenotype, enhanceable by Pvr^GD14375, Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

Ptth^120F2A has abnormal developmental rate phenotype, enhanceable by Scer\GAL4^phtm.PO/Pvr^GD14375

(Pan and O'Connor, 2021)

Ptth^120F2A has abnormal developmental rate phenotype, enhanceable by Scer\GAL4^NP0423/jeb^HMC04318

(Pan and O'Connor, 2021)

Ptth^120F2A has abnormal developmental rate phenotype, enhanceable by Alk^JF02668/Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

NOT Enhanced by

Statement

Reference

Alk^JF02668, Ptth^120F2A, Scer\GAL4^phtm.PO has increased body size | P-stage phenotype, non-enhanceable by Pvr^GD14375, Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

Ptth^120F2A, Scer\GAL4^NP0423, jeb^HMC04318 has increased body size | P-stage phenotype, non-enhanceable by Pvf3^NIG.13781R, Scer\GAL4^NP0423

(Pan and O'Connor, 2021)

Ptth^120F2A, Scer\GAL4^NP0423, jeb^HMC04318 has abnormal developmental rate phenotype, non-enhanceable by Pvf3^NIG.13781R, Scer\GAL4^NP0423

(Pan and O'Connor, 2021)

Suppressed by

Statement

Reference

Ptth^120F2A has abnormal developmental rate phenotype, suppressible by Scer\GAL4^spok.1.45/Pvr^UAS.cDa

(Pan and O'Connor, 2021)

Ptth^120F2A has abnormal developmental rate | larval stage phenotype, suppressible by Scer\GAL4^Ptth.cMa/TrpA1^UAS.(B).cKa

(Shimell et al., 2018)

NOT suppressed by

Statement

Reference

Alk^JF02668, Ptth^120F2A, Scer\GAL4^phtm.PO has increased body size | P-stage phenotype, non-suppressible by Pvr^GD14375, Scer\GAL4^phtm.PO

(Pan and O'Connor, 2021)

Ptth^120F2A, Scer\GAL4^NP0423, jeb^HMC04318 has increased body size | P-stage phenotype, non-suppressible by Pvf3^NIG.13781R, Scer\GAL4^NP0423

(Pan and O'Connor, 2021)

Ptth^120F2A, Scer\GAL4^NP0423, jeb^HMC04318 has abnormal developmental rate phenotype, non-suppressible by Pvf3^NIG.13781R, Scer\GAL4^NP0423

(Pan and O'Connor, 2021)

Ptth^120F2A/Ptth^Delta has abnormal developmental rate | larval stage phenotype, non-suppressible by nvd^UAS.cYa/Scer\GAL4^P0206

(Shimell et al., 2018)

Ptth^120F2A/Ptth^Delta has abnormal developmental rate | larval stage phenotype, non-suppressible by Scer\GAL4^P0206/spok^UAS.cOa

(Shimell et al., 2018)

Ptth^120F2A has abnormal developmental rate | larval stage phenotype, non-suppressible by Scer\GAL4^P0206/dib^UAS.cOa

(Shimell et al., 2018)

Ptth^120F2A has abnormal developmental rate | larval stage phenotype, non-suppressible by Scer\GAL4^spok.1.45/Cyp6t3^UAS.cDa

(Shimell et al., 2018)

Enhancer of

Statement

Reference

Ptth^120F2A is an enhancer of abnormal developmental rate | larval stage phenotype of Hsap\KCNJ2^UAS.EGFP, Scer\GAL4^Ptth.cMa

(Shimell et al., 2018)

Suppressor of

Statement

Reference

Ptth^120F2A is a suppressor of abnormal developmental rate | larval stage phenotype of Scer\GAL4^Ptth.cMa, TrpA1^UAS.(B).cKa

(Shimell et al., 2018)

Other

Statement

Reference

Ptth^120F2A, Pvr^GD14375, Scer\GAL4^phtm.PO has increased body size | P-stage phenotype

(Pan and O'Connor, 2021)

Ptth^120F2A, Pvr^UAS.cDa, Scer\GAL4^spok.1.45 has decreased body size | P-stage phenotype

(Pan and O'Connor, 2021)

Alk^JF02668, Ptth^120F2A, Scer\GAL4^phtm.PO has partially lethal phenotype

(Pan and O'Connor, 2021)

Alk^JF02668, Ptth^120F2A, Scer\GAL4^phtm.PO has increased body size | P-stage phenotype

(Pan and O'Connor, 2021)

Alk^{act.UAS.Tag:HA}, Ptth^120F2A, Scer\GAL4^spok.1.45 has abnormal developmental rate phenotype

(Pan and O'Connor, 2021)

Alk^{act.UAS.Tag:HA}, Ptth^120F2A, Scer\GAL4^spok.1.45 has decreased body size | P-stage phenotype

(Pan and O'Connor, 2021)

Ptth^120F2A, Scer\GAL4^NP0423, jeb^HMC04318 has increased body size | P-stage phenotype

(Pan and O'Connor, 2021)

Ptth^120F2A, gt^E1 has abnormal size | larval stage phenotype

(Shimell et al., 2018)

Phenotype Manifest In

Other

Statement

Reference

Ptth^120F2A, gt^E1 has prothoracic gland phenotype

(Shimell et al., 2018)

Ptth^120F2A, gt^E1 has nucleus | larval stage phenotype

(Shimell et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The nuclei of innervated prothoracic gland lobes in gt^E1/gt^E1, Ptth^120F2A/Ptth^120F2A do not show the expected increased size as compared to non-innervated lobes; gt^E1/gt^E1, Ptth^120F2A/+ individuals, however, show that expected increase.

Ptth^120F2A mutants with activated Scer\GAL4^Ptth.cMa-expressing prothoracic gland neurons (using TrpA1^UAS.(B).cKa) show normal developmental time to pupariation, instead of the delay observed in Ptth^120F2A mutants or the acceleration induced by Scer\GAL4^Ptth.cMa neurons activation.

(Shimell et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ptth^120F2A is rescued by Ptth^Tag:HA

(Shimell et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

606367

y¹ w^*; Ptth^120F2A

606369

w^*; Ptth^120F2A P{GAL4}P0206

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ptth^120F2A

(Shimell et al., 2018)

ptth^120F2A

(Pan and O'Connor, 2021, Shimell and O'Connor, 2019)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

Open Close