FlyBase Allele Report: Dmel\Ptth[Delta]

General Information

Symbol

Dmel\Ptth^Delta

Species

D. melanogaster

Name

FlyBase ID

FBal0345092

Feature type

allele

Associated gene

Dmel\Ptth

Associated Insertion(s)

TI{mCherry}Ptth^Delta

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

gene targeting by homologous recombination

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Shimell et al., 2018)

Mutagen

gene targeting by homologous recombination

(Shimell et al., 2018)

Progenitor genotype

Associated Insertion(s)

TI{mCherry}Ptth^Delta

Cytology

Description

600bp of Ptth has been deleted and replaced by a sequence cassette.

(Romero, 2019.3.26)

Part of Ptth, including the putative ATG codon and the signal peptide, has been deleted. The following sequence (originating from the pTV-Cherry-based donor plasmid) has been inserted in place of the deleted Ptth sequence: a single attP site and a loxP cassette which itself contains an mFRT71 cassette followed by Scer\GAL4 coding sequence and a polyadenylation signal (this GAL4 sequence is non-functional). The mFRT71 cassette contains 1: the mCherry coding region tagged with a membrane-targeting signal (Tag:Myr(Unk)) and followed by a polyadenylation signal, 2: a w^+* marker.

(Shimell et al., 2018)

Allele components

Component

Use(s)

Inserted element

TI{mCherry}

promoter trap

Encoded product / tool

mCherry

red fluorescent protein

Also carries

attP

integrase target site

loxP

recombinase target site

mFRT71

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

2L:576,323..576,922 [-]

Comment:

Reported as a 604bp deletion that removes the Ptth ATG and signal sequence. The approximate mapped boundaries of the deletion were inferred from the reported primer sequences and extend 600 bases. This region was replaced with sequences from a pTV-Cherry-based donor plasmid.

Comment:

The deletion was reported as a 604bp deletion but based on the primers used, maps to 2L:576323..576922 , a 600bp deletion. The deleted region was replaced with sequences from a pTV-Cherry-based donor plasmid.

(Romero, 2019.3.26, Shimell et al., 2018)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

ovariole

(Shimell et al., 2018)

wing

(Shimell et al., 2018)

wing disc | third instar larval stage

(Shimell et al., 2018)

Detailed Description

Statement

Reference

Ptth^Delta mutants exhibit increased larva-to-adult mortality either in a poor diet or in crowded conditions in normal diet, as compared to controls.

Ptth^Delta and Ptth^Delta/Ptth^120F2A mutants exhibit a 1-day delay in the developmental time from L1 to pupariation, as compared to controls. Ptth^Delta mutants show a more pronounced delay under poor diet (poor yeast extract) than under normal food.

In third instar larvae, wing discs grow significantly more slowly but are significantly larger at the onset of metamorphosis, as compared to controls; the brain, wing disc nuclei, body nuclei and salivary gland nuclei are of comparable size to controls.

Ptth^Delta adults show significantly larger wings; males are significantly heavier than controls; females show significantly more ovarioles than controls, even in crowded conditions.

(Shimell et al., 2018)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Ptth^120F2A/Ptth^Delta has abnormal developmental rate | larval stage phenotype, non-suppressible by nvd^UAS.cYa/Scer\GAL4^P0206

(Shimell et al., 2018)

Ptth^120F2A/Ptth^Delta has abnormal developmental rate | larval stage phenotype, non-suppressible by Scer\GAL4^P0206/spok^UAS.cOa

(Shimell et al., 2018)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Ptth^Delta is rescued by Scer\GAL4^Tub.PU/Ptth^UAS.Tag:HA

(Shimell et al., 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Ptth^Delta

(Shimell et al., 2018)

ptth^delta

(Cavieres-Lepe et al., 2024)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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