FlyBase Allele Report: Dmel\Csk[c04256]

General Information

Symbol

Dmel\Csk^c04256

Species

D. melanogaster

Name

FlyBase ID

FBal0160682

Feature type

allele

Associated gene

Dmel\Csk

Associated Insertion(s)

PBac{PB}Csk^c04256

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{PB}Csk^c04256

Cytology

Description

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:11,650,632..11,650,632 [+]

(Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates viral infectious disease

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Bloomington Drosophila Stock Center, 2004.11.6)

viable

(Bloomington Drosophila Stock Center, 2004.11.6)

visible | somatic clone

(Vidal et al., 2006)

Phenotype Manifest In

eye | somatic clone

(Vidal et al., 2006)

ommatidium | somatic clone

(Vidal et al., 2006)

Detailed Description

Statement

Reference

Csk^c04256/Csk^c04256 third instar larvae show significant decreases in mEPSP (decreasing quantal size) and significant increases in quantal content in response to application of the glutamate receptor blocker PhTox for 10 minutes at the neuromuscular junction, similar to wild type. Csk^c04256/Csk^c04256 third instar larvae do not show significant differences in the number of boutons per muscle area at the neuromuscular junction (A2 6/7 and A3 6/7) compared to wild type.

Csk^c04256/Csk^c04256 or Csk^c04256/Csk^j1D8 third instar larvae do not show significant neurotransmission defects (mEPSPs, EPSPs and quantal content similar to wild type). Csk^c04256/Csk^c04256 third instar larvae are similar to wild type in their baseline probability of release and show normal calcium cooperativity of release.

(Spring et al., 2016)

Eyes made entirely of Csk^c04256/Csk^c04256 somatic clone cells are rough and enlarged.

(Vidal et al., 2006)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Csk^j1D8/Csk^c04256, Fas2^e76, GluRIIA^SP16 has abnormal neuroanatomy | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, Fas2^e76, GluRIIA^SP16 has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^UAS.YFP, Csk^j1D8/Csk^c04256, GluRIIA^SP16, Scer\GAL4^nau.PM has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^c04256, GluRIIA^SP16 has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16 has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16, Src64B[+]/Src64B^ko has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Csk^c04256, GluRIIA^SP16 has abnormal neuroanatomy | third instar larval stage phenotype

(Spring et al., 2016)

Phenotype Manifest In

Enhancer of

Statement

Reference

Csk^c04256 is an enhancer of eye phenotype of EBV\BRLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Other

Statement

Reference

Csk^c04256, GluRIIA^SP16 has NMJ bouton | third instar larval stage phenotype

(Spring et al., 2016)

Csk^UAS.YFP, Csk^j1D8/Csk^c04256, GluRIIA^SP16, Scer\GAL4^nau.PM has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, Fas2^e76, GluRIIA^SP16 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^c04256, GluRIIA^SP16 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Csk^j1D8/Csk^c04256, GluRIIA^SP16, Src64B[+]/Src64B^ko has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Third instar larval GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^c04256 double mutants show significant decreases in mEPSP (decreasing quantal size) but do not show significant increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; double mutants show diminished evoked muscle excitation compared to GluRIIA^SP16/GluRIIA^SP16 larvae.

GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^c04256 third instar larvae do not show significant differences in the number of boutons per muscle area at the neuromuscular junction (A2 6/7 and A3 6/7) compared to wild type, though do show a significant decrease at A3 6/7 compared to Csk^c04256/Csk^c04256 larvae; active zone number is increased (though not significantly) at A3 6/7 in double mutants, compared to GluRIIA^SP16/GluRIIA^SP16 larvae.

GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^c04256 third instar larvae do not deplete evoked responses to the same degree as GluRIIA^SP16/GluRIIA^SP16 larvae over the course of a lengthy high frequency stimulus paradigm (10Hz X 6000 pulses = 10 minutes) in high extracellular calcium (2mM) at the NMJ.

GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^c04256 Src42A^k10108/+ third instar larvae show significant decreases in mEPSP (decreased quantal size) but do not show significant compensatory homeostatic increases in quantal content (similar to GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^c04256 double mutants) at the NMJ.

GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^j1D8 Src64B^ko/+ third instar larvae show significant decreases in mEPSP (decreased quantal size) partially restores significant compensatory homeostatic increases in quantal content (unlike GluRIIA^SP16/GluRIIA^SP16 Csk^c04256/Csk^j1D8 double mutants, which do not show significant increases in quantal content) at the NMJ.

GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 third instar larvae show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; additional expression of Csk^{Scer\UAS.T:Avic\GFP-YFP} driven by Scer\GAL4^nau.PM or Scer\GAL4^VGlut-OK371 restores phenotypes to those seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone.

GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 third instar larvae show significant decreases in mEPSP (decreasing quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ; additional presence of Fas2^e76 restores phenotypes to those seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone. GluRIIA^SP16/GluRIIA^SP16;Csk^c04256/Csk^j1D8 Fas2^e76 third instar larvae also show a small significant increase in bouton number per muscle area (segment A2, synapse 6/7).

(Spring et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11400

w¹¹¹⁸; PBac{PB}Csk^c04256

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Csk^c04256

(Spring et al., 2016, Adamson and Lajeunesse, 2012)

dCsk^c04256

(Vidal et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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