FlyBase Allele Report: Dmel\spz5[e03444]

General Information

Symbol

Dmel\spz5^e03444

Species

D. melanogaster

Name

FlyBase ID

FBal0160745

Feature type

allele

Associated gene

Dmel\spz5

Associated Insertion(s)

PBac{RB}spz5^e03444

Carried in Construct

Also Known As

DNT2^e03444

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{RB}spz5^e03444

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{RB}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:2,887,990..2,887,990 [-]

(Zhang et al., 2024, McIlroy et al., 2013, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates nervous system disease

modeled by Atpα^CJ10

(Talsma et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with Df(3L)Exel6092)

(Zhu et al., 2008)

abnormal neuroanatomy | adult stage (with Df(3L)Exel6092)

(Zhang et al., 2024)

fertile

(Zhu et al., 2008, Bloomington Drosophila Stock Center, 2004.11.6)

increased cell death (with Df(3L)Exel6092)

(Zhu et al., 2008)

viable

(McIlroy et al., 2013, Zhu et al., 2008, Bloomington Drosophila Stock Center, 2004.11.6)

Phenotype Manifest In

adult dopaminergic neuron | decreased number (with Df(3L)Exel6092)

(Zhang et al., 2024)

larval intersegmental nerve branch ISNb of A1-7 (with Df(3L)Exel6092)

(Zhu et al., 2008)

larval intersegmental nerve branch ISNd of A1-7 (with Df(3L)Exel6092)

(Zhu et al., 2008)

larval segmental nerve branch SNa of A1-7 (with Df(3L)Exel6092)

(Zhu et al., 2008)

Detailed Description

Statement

Reference

spz5^e03444/Df(3L)Exel6092 transheterozygous mutant embryos exhibit an increase in apoptosis.

Both ISNb/d and SNa projections are mildly affected in spz5^e03444/Df(3L)Exel6092 mutant embryos.

(Zhu et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

spz5^e03444, spz² has abnormal neuroanatomy phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has abnormal neuroanatomy phenotype, enhanceable by spz²/NT1⁴¹

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has abnormal neuroanatomy phenotype, enhanceable by spz²

(Zhu et al., 2008)

spz5^e03444 has abnormal neuroanatomy phenotype, enhanceable by Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

Suppressed by

Statement

Reference

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by Scer\GAL4^elav.PU/kek2^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by Scer\GAL4^elav.PU/kek3^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by kek5^{UAS.mRFP(Unk)}/Scer\GAL4^elav.PU

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, suppressible by Scer\GAL4^elav.PU/kek6^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Toll-6^{ΔLRR.UAS.Tag:HA}

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Toll-7^{C993Y.UAS.Tag:FLAG}/Scer\GAL4^elav-C155

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Toll-7^{ΔLRR.UAS.Tag:HA}

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype, suppressible by Scer\GAL4^elav-C155/Tl^10b.UAS.cYa

(McIlroy et al., 2013)

NOT suppressed by

Statement

Reference

NT1⁴¹, spz5^e03444 has lethal phenotype, non-suppressible by Scer\GAL4^elav.PU/kek1^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has lethal phenotype, non-suppressible by Scer\GAL4^elav.PU/kek4^{UAS.mRFP(Unk)}

(Ulian-Benitez et al., 2017)

Enhancer of

Statement

Reference

NT1⁴¹/spz5^e03444 is an enhancer of abnormal neuroanatomy phenotype of spz²

(Zhu et al., 2008)

spz5^e03444 is an enhancer of abnormal neuroanatomy phenotype of Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of abnormal neuroanatomy phenotype of NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 is an enhancer of abnormal neuroanatomy phenotype of NT1⁴¹, spz²

(Zhu et al., 2008)

Other

Statement

Reference

NT1⁴¹, spz5^e03444 has lethal phenotype

(Ulian-Benitez et al., 2017)

NT1⁴¹, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype

(McIlroy et al., 2013)

Toll-6²⁶, spz5^e03444 has viable phenotype

(McIlroy et al., 2013)

Toll-7^P114, spz5^e03444 has some die during embryonic stage | heat sensitive phenotype

(McIlroy et al., 2013)

NT1⁴¹, spz5^e03444, spz² has abnormal locomotor rhythm phenotype

(Zhu et al., 2008)

Df(3L)ED4342/NT1⁴¹, spz5^e03444, spz² has abnormal locomotor rhythm phenotype

(Zhu et al., 2008)

Phenotype Manifest In

Enhanced by

Statement

Reference

spz5^e03444, spz² has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz5^e03444, spz² has larval segmental nerve phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz5^e03444, spz² has larval transverse nerve phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by spz²/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by spz²/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has larval segmental nerve branch SNa of A1-7 phenotype, enhanceable by spz²/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has larval segmental nerve phenotype, enhanceable by spz²/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has larval transverse nerve phenotype, enhanceable by spz²/NT1⁴¹

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by spz²

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by spz²

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval segmental nerve phenotype, enhanceable by spz²

(Zhu et al., 2008)

NT1⁴¹, spz5^e03444 has larval transverse nerve phenotype, enhanceable by spz²

(Zhu et al., 2008)

spz5^e03444 has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has larval intersegmental nerve branch ISNd of A1-7 phenotype, enhanceable by Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 has larval segmental nerve phenotype, enhanceable by Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444, spz² has larval intersegmental nerve branch ISNb of A1-7 phenotype, enhanceable by NT1⁴¹

(Zhu et al., 2008)

Enhancer of

Statement

Reference

spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval segmental nerve phenotype of spz²

(Zhu et al., 2008)

NT1⁴¹/spz5^e03444 is an enhancer of larval transverse nerve phenotype of spz²

(Zhu et al., 2008)

spz5^e03444 is an enhancer of larval segmental nerve phenotype of Df(3L)ED4342/NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval segmental nerve branch SNa of A1-7 phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval segmental nerve phenotype of NT1⁴¹

(Zhu et al., 2008)

spz²/spz5^e03444 is an enhancer of larval transverse nerve phenotype of NT1⁴¹

(Zhu et al., 2008)

spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNb of A1-7 phenotype of NT1⁴¹, spz²

(Zhu et al., 2008)

spz5^e03444 is an enhancer of larval intersegmental nerve branch ISNd of A1-7 phenotype of NT1⁴¹, spz²

(Zhu et al., 2008)

spz5^e03444 is an enhancer of larval segmental nerve phenotype of NT1⁴¹, spz²

(Zhu et al., 2008)

spz5^e03444 is an enhancer of larval transverse nerve phenotype of NT1⁴¹, spz²

(Zhu et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

NT1⁴¹, spz5^e03444 double homozygosity leads to near lethality, which is rescued by Scer\GAL4^elav.PU-driven expression of kek1^{UAS.mRFP(Unk)}, kek3^{UAS.mRFP(Unk)}, kek5^{UAS.mRFP(Unk)} (leading to a small but significant increase in viability), kek2^{UAS.mRFP(Unk)} or kek6^{UAS.mRFP(Unk)} (leading to near full viability), but not kek4^{UAS.mRFP(Unk)}.

(Ulian-Benitez et al., 2017)

Loss of both NT1 and spz5 in spz5^e03444 NT1⁴¹ double-mutant embryos results in an increase in apoptosis compared to each of the single mutants, revealing redundant or complementary functions in the control of neuronal survival.

Viability is affected in NT1⁴¹ spz5^e03444 double-mutants: in homozygosis, double mutant flies are viable (although some larval lethality, as well as when in trans over NT1⁴¹ Df(3L)Exel6092, is observed), but NT1⁴¹ spz5^e03444/In(3LR)TM6B flies do not produce homozygous progeny at 18[o]C, suggesting unsuccessful larval competition.

The penetrance of ISNb/d targeting defects increases in spz5^e03444 NT1⁴¹ / Df(3L)Exel6092 NT1⁴¹ double-mutant embryos, although not significantly.

The penetrance of both SNa and ISNb/d targeting defects increases in spz5^e03444 NT1⁴¹ spz², compared to the double or single mutants. In the triple mutants, misrouting phenotypes can be very dramatic, and there are cases of loss of all ISNb/d motor axons (not seen in single mutants). Misrouting of the transverse nerve (TN) can be very dramatic in triple mutants, although milder effects in this nerve occur with comparable penetrance in all genotypes (~10%). Misroutings of ISN are negligible in single and double mutants, but they increase and can be dramatic in triple-mutant embryos (12.7%).

NT1⁴¹ spz5^e03444 / NT1⁴¹ Df(3L)Exel6092 double mutant flies display a range of behavioral phenotypes including: inability to estimate the location of a petri dish rim, falling off a petri dish rim, sluggishness, inability to climb, and wobbling.

(Zhu et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

18155

w¹¹¹⁸; PBac{RB}spz5^e03444

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG9972^e03444

DNT2^e03444

(Ulian-Benitez et al., 2017, McIlroy et al., 2013, Zhu et al., 2008)

Spz-5^e03444

(Zhang et al., 2024)

spz5^e03444

(Aw et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections