FlyBase Allele Report: Dmel\Mctp[e00415]

General Information

Symbol

Dmel\Mctp^e00415

Species

D. melanogaster

Name

FlyBase ID

FBal0161543

Feature type

allele

Associated gene

Dmel\Mctp

Associated Insertion(s)

PBac{RB}Mctp^e00415

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{RB}Mctp^e00415

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{RB}

gene trap

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:18,774,388..18,774,388 [-]

(Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | third instar larval stage

(Genç et al., 2017)

abnormal neurophysiology | third instar larval stage

(Genç et al., 2017)

fertile

(Bloomington Drosophila Stock Center, 2004.11.6)

viable

(Genç et al., 2017, Bloomington Drosophila Stock Center, 2004.11.6)

Phenotype Manifest In

embryonic/larval neuromuscular junction | third instar larval stage

(Genç et al., 2017)

NMJ bouton | third instar larval stage

(Genç et al., 2017)

Detailed Description

Statement

Reference

Recordings in Mctp^e00415 mutant third instar larval neuromuscular junctions under standard conditions show significantly decreased EPSP amplitudes and quantal content, but no changes in mEPSP amplitudes, as compared to controls. These neuromuscular junctions are mostly normal (normal number/density of active zones) but show a small but significant increase in bouton number.

(Genç et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155, Mctp^e00415, Mctp^UAS.cGa is a non-enhancer of abnormal neurophysiology | third instar larval stage phenotype of GluRIIA^unspecified

(Genç et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155, Mctp^e00415, Mctp^UAS.cGa is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of GluRIIA^unspecified

(Genç et al., 2017)

Other

Statement

Reference

GluRIIA^unspecified, Mctp^e00415 has abnormal neurophysiology | third instar larval stage phenotype

(Genç et al., 2017)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

Scer\GAL4^elav-C155, Mctp^e00415, Mctp^UAS.cGa is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of GluRIIA^unspecified

(Genç et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155, Mctp^e00415, Mctp^UAS.cGa is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of GluRIIA^unspecified

(Genç et al., 2017)

Other

Statement

Reference

GluRIIA^unspecified, Mctp^e00415 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Genç et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Although both GluRIIA^unspecified single mutants and in GluRIIA^unspecified, Mctp^e00415 double mutants show similarly decreased mEPSP amplitudes in the third instar larval neuromuscular junction, double mutants show a decrease in quantal content instead of the homeostatic increase in quantal content, and also show a decrease in EPSP amplitude; Mctp^UAS.cGa expression under the control of Scer\GAL4^elav-C155 in the double mutant background leads to homeostatic increase in quantal content as observed in GluRIIA^unspecified single mutants.

(Genç et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mctp^e00415 is rescued by Scer\GAL4^elav-C155/Mctp^UAS.cGa

(Genç et al., 2017)

Mctp^e00415 is rescued by Mctp^UAS.cGa/Scer\GAL4^Mhc.PU

(Genç et al., 2017)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17855

w¹¹¹⁸; PBac{RB}Mctp^e00415

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG33146^e00415

(Gene Disruption Project members and Exelixis, 2005)

Mctp^e00415

Pbac^e00415

(Genç et al., 2017)

mctp¹

(Genç et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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