FlyBase Allele Report: Dmel\ppk29[f06838]

General Information

Symbol

Dmel\ppk29^f06838

Species

D. melanogaster

Name

FlyBase ID

FBal0162356

Feature type

allele

Associated gene

Dmel\ppk29

Associated Insertion(s)

PBac{WH}ppk29^f06838

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

piggyBac activity

Nature of the Allele

Allele class

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}ppk29^f06838

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:24,054,313..24,054,313 [-]

(Vijayan et al., 2014, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

motor neuron | larval stage | heat sensitive

(Zheng et al., 2014)

Detailed Description

Statement

Reference

Mutant flies are less sensitive than wild-type flies to acute heat stress, with the time to total paralysis being significantly longer in the mutant flies than in wild-type controls.

At 38[o]C, motor neurons of mutant larvae are unable to increase their firing rate in response to heat stress, in contrast to wild-type controls. The mutant larvae do not show abnormal seizure-like locomotion at this temperature, in contrast to that seen in wild-type controls.

(Zheng et al., 2014)

Homozygous and ppk29^f06838/Df(2R)BSC136 males show significantly reduced levels of courtship (both courtship index and the fraction of males initiating courtship in the presence of decapitated females) compared to wild-type males.

Homozygous males do not show significant levels of courtship towards other males.

(Liu et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

ppk29^f06838/ppk29^f04205 has abnormal courtship behavior | male phenotype, non-suppressible by Scer\GAL4^ppk29.3.020/ppk25^UAS.cSa

(Liu et al., 2012)

ppk29^f06838/ppk29^f04205 has abnormal courtship behavior | male phenotype, non-suppressible by Scer\GAL4^ppk25.PS/ppk25^UAS.cSa

(Liu et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of ppk25^Scer\UAS.cSa under the control of either Scer\GAL4^ppk29.3.020 or Scer\GAL4^ppk25.PS does not rescue the reduced courtship index of ppk29^f06838/ppk29^f04205 males.

(Liu et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

ppk29^f06838 is rescued by ppk29^3'UTR.UAS/Scer\GAL4^elav-C155

(Zheng et al., 2014)

ppk29^f06838/ppk29^f04205 is rescued by ppk29^UAS.cLa/Scer\GAL4^ppk29.3.020

(Liu et al., 2012)

ppk29^f06838/ppk29^f04205 is rescued by ppk29^UAS.cLa/Scer\GAL4^ppk25.PS

(Liu et al., 2012)

Partially rescued by

ppk29^f06838 is partially rescued by Scer\GAL4^elav-C155/ppk29^Δ3'UTR.UAS

(Zheng et al., 2014)

ppk29^f06838 is partially rescued by Scer\GAL4^elav-C155/Disc\RFP^{mCherry.ppk29.3'UTR.UAS}

(Zheng et al., 2014)

ppk29^f06838/ppk29^f04205 is partially rescued by ppk29^UAS.cLa

(Liu et al., 2012)

Not rescued by

ppk29^f06838/ppk29^f04205 is not rescued by Scer\GAL4^ppk29.3.020/ppk29^UAS.alt

(Liu et al., 2012)

ppk29^f06838/ppk29^f04205 is not rescued by Scer\GAL4^ppk25.PS/ppk29^UAS.alt

(Liu et al., 2012)

Comments

The increased time to total paralysis in response to heat stress which is seen in ppk29^f06838 flies is fully rescued by expression of ppk29^{3'UTR.Scer\UAS} under the control of Scer\GAL4^elav-C155.

The increased time to total paralysis in response to heat stress which is seen in ppk29^f06838 flies is partially rescued by expression of either ppk29^{Δ3'UTR.Scer\UAS} or Disc\RFP^{mCherry.ppk29.3'UTR} under the control of Scer\GAL4^elav-C155.

(Zheng et al., 2014)

Expression of ppk29^Scer\UAS.cLa under the control of either Scer\GAL4^ppk29.3.020 or Scer\GAL4^ppk25.PS rescues the reduced courtship index of ppk29^f06838/ppk29^f04205 males. Partial rescue is seen in the absence of a Scer\GAL4 driver.

Expression of ppk29^Scer\UAS.alt under the control of either Scer\GAL4^ppk29.3.020 or Scer\GAL4^ppk25.PS does not rescue the reduced courtship index of ppk29^f06838/ppk29^f04205 males.

(Liu et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

19016

w¹¹¹⁸; PBac{WH}ppk29^f06838

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG13568^f06838

f06838

(Liu et al., 2012)

nope¹

(Liu et al., 2012)

ppk29^P1

(Zheng et al., 2014)

ppk29^f06838

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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