FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\nejRNAi.UAS.cKa
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General Information
Symbol
Dmel\nejRNAi.UAS.cKa
Species
D. melanogaster
Name
FlyBase ID
FBal0175570
Feature type
allele
Associated gene
Dmel\nej
Associated Insertion(s)
Carried in Construct
P{UAS-nej.RNAi.K}
Also Known As
UAS-CBP RNAi
Key Links
Transgenic product class
RNAi_reagent
(Kumar et al., 2004)
Mutagen
Nature of the Allele
Transgenic product class
RNAi_reagent
(Kumar et al., 2004)
Mutagen
in vitro construct
(Kumar et al., 2004)
Progenitor genotype
Carried in construct
P{UAS-nej.RNAi.K}
Cytology
Description

UASt regulatory sequences drive expression of an RNAi "snapback" construct consisting of an inverted repeat of a 697bp nej fragment (269bp of 5'UTR and 428bp of 5' coding sequence).

(Kumar et al., 2004)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
nej
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 1 )
      Disease
      Interaction
      References
      exacerbates  Machado-Joseph disease
      modeled by Hsap\ATXN3tr.Q78.UAS.Tag:HA
      (Raj and Sarkar, 2019)
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of nejdsRNA.Scer\UAS.cKa under the control of Scer\GAL4bbg-C96 produces wrinkles and notches in the wing blade.

      Expression of nejdsRNA.Scer\UAS.cKa under the control of Scer\GAL4pnr-MD237 engenders macrochaeta abnormalities on the scutellum.

      Expression of nejdsRNA.Scer\UAS.cKa under the control of Scer\GAL4GMR.PF engenders a roughened appearance of the eye.

      Heat shocked flies expressing nejdsRNA.Scer\UAS.cKa under the control of Scer\GAL4hs.PU display impaired geotactic response compared with controls.

      (Boyles et al., 2010)

      Scer\GAL4GMR.PF-mediated expression of nejdsRNA.Scer\UAS.cKa results in depigmented and somewhat smooth eyes with individual facets being less distinct, especially in the posterior region.

      Scer\GAL4Act5C.PI-mediated expression of nejdsRNA.Scer\UAS.cKa causes some degree of embryonic and larval lethality. All surviving larvae pupate, but die as early or differentiated pupae and none eclose.

      (Mallik and Lakhotia, 2010)

      42% of animals expressing nejdsRNA.Scer\UAS.cKa under the control of Scer\GAL4hs.PB at 29[o]C show partial transformation of abdominal segment A5 to A4.

      (Tie et al., 2009)

      Flies expressing nejdsRNA.Scer\UAS.cKa under the control of Scer\GAL4GMR.PF have eyes which are relatively smooth externally (individual facets cannot be distinguished and the number of interommatidial bristles is dramatically reduced). Cross sections through the retina confirm the loss of many ommatidia, but the majority of the retina has photoreceptor clusters. Each ommatidial cluster that is present appears to have fewer than the normal number of photoreceptor cells and the remaining photoreceptor cells appear to have defective rhabdomeres. Expression of nejdsRNA.Scer\UAS.cKa under the control of Scer\GAL4ey.PH inhibits eye development; in larvae, the eye disc is smaller and contains fewer ommatidial clusters than normal.

      (Kumar et al., 2004)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Suppressor of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Suppressed by
      Enhancer of
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of P{Sym-UAS-Hsrω} in the Scer\GAL4GMR.PF, nejdsRNA.Scer\UAS.cKa background significantly improves pigmentation and eye morphology, including in the posterior part. In contrast, co-expression of HsrωEP3037 or HsrωEP93D enhances eye degeneration with the loss of ommatidial integrity extending to most of the eye.

      More embryos/larvae die when nejdsRNA.Scer\UAS.cKa and P{Sym-UAS-Hsrω} are co-expressed with Scer\GAL4Act5C.PI than when either is expressed alone. Moreover, co-expression of P{Sym-UAS-Hsrω} in the Scer\GAL4Act5C.PI, nejdsRNA.Scer\UAS.cKa background results in eclosing pupae, whereas no pupae eclose when P{Sym-UAS-Hsrω} is expressed alone.

      (Mallik and Lakhotia, 2010)
      Xenogenetic Interactions
      Statement
      Reference

      Co-expression of nejdsRNA.Scer\UAS.cKa fails to suppress the small eye and lens fusion phenotype seen in adult flies expressing Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU. Co-expression of nejdsRNA.Scer\UAS.cKa partially suppresses the increased cell death observed in third instar eye discs of flies expressing Hsap\APPAβ42.Scer\UAS.cUa under the control of Scer\GAL4GMR.PU.

      (Cutler et al., 2015)

      Expression of nejdsRNA.Scer\UAS.cKa in the Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 background results in >97% lethality at the pharate stage with eye degeneration being dramatically enhanced. Additional co-expression of P{Sym-UAS-Hsrω} robustly suppresses this enhanced eye damage, and also substantially reverses Scer\GAL4GMR.PF, Zzzz\CAG127Q.Scer\UAS.T:Ivir\HA1 eye degeneration in a dose-dependent manner. Co-expression of P{Sym-UAS-Hsrω} also improves emergence to adult stage in a dose-dependent manner.

      Expression of nejdsRNA.Scer\UAS.cKa in the Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 background results in >97% lethality at the pharate stage with eye degeneration being dramatically enhanced. Additional co-expression of P{Sym-UAS-Hsrω} robustly suppresses this enhanced eye damage, and also substantially reverses Scer\GAL4GMR.PF, Hsap\MJDtr.Q78.Scer\UAS.T:Ivir\HA1 eye degeneration in a dose-dependent manner. Co-expression of P{Sym-UAS-Hsrω} also improves emergence to adult stage in a dose-dependent manner.

      (Mallik and Lakhotia, 2010)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      CBPRNAi
      (Cutler et al., 2015)
      nejRNAi.UAS.cKa
      nejdsRNA.Scer\UAS.cKa
      nejdsRNA.UAS.cKa
      Name Synonyms
      Secondary FlyBase IDs
        References (11)