FlyBase Allele Report: Dmel\nej[ΔNZK.UAS]

General Information

Symbol

Dmel\nej^ΔNZK.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0175576

Feature type

allele

Associated gene

Dmel\nej

Associated Insertion(s)

Carried in Construct

P{UAS-nej.ΔNZK}

Also Known As

UAS-CBP ΔNZK

Key Links

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Kumar et al., 2004)

Mutagen

in vitro construct

(Kumar et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-nej.ΔNZK}

Cytology

Description

UASt regulatory sequences drive expression of a deleted form of nej, consisting of an ATG followed by nej amino acids 1030-3275.

(Kumar et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

nej

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Alzheimer's disease

modeled by Hsap\APP^{Aβ42.UAS.cUa}

(Cutler et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^ey.PH

(Kumar et al., 2004)

visible, with Scer\GAL4^GMR.PF

(Anderson et al., 2005, Kumar et al., 2004)

visible, with Scer\GAL4^GMR.PF, nej³

(Kumar et al., 2004)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010, Anderson et al., 2005, Kumar et al., 2004)

eye, with Scer\GAL4^GMR.PF, nej³

(Kumar et al., 2004)

eye | dorsal, with Scer\GAL4^ey.PH

(Kumar et al., 2004)

eye disc, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Anderson et al., 2005)

eye photoreceptor cell | ectopic, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010, Anderson et al., 2005, Kumar et al., 2004)

mystery cell, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

ommatidial precursor cluster, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Kumar et al., 2004)

Detailed Description

Statement

Reference

Expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^GMR.PU does not result in any obvious eye phenotypes in adults.

(Cutler et al., 2015)

Flies expressing nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^GMR.PF exhibit a slight roughening of the external retinal surface and disruption of the ommatidial bristle arrangement because of missing or extra bristles at some places.

(Mallik and Lakhotia, 2010)

Expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in a lack of interommatidial bristles. In addition, each ommatidium appears to contain one to two extra photoreceptor cells.

(Anderson et al., 2005)

Expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^GMR.PF results in adults with a slight roughening of the external surface of the eye and a loss of interommatidial bristles. Pupal retinas contain a normal complement of accessory cone and pigment cells. Sections through the adult retina show that ommatidia contain one or two extra photoreceptor cells per ommatidium, and this phenotype can also be seen in the larval eye imaginal disc. These extra photoreceptor cells are in the right position to be the "mystery cells" being recruited into the ommatidium. Expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^hs.2sev appears to have no effect on eye development. Expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^ey.PH strongly inhibits eye development in the dorsal half of the eye, while pattern formation proceeds in the ventral domain. The moderate rough eye phenotype caused by expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^GMR.PF is enhanced if the flies also carry nej³/+.

(Kumar et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

nej^ΔNZK.UAS, Scer\GAL4^GMR.PU is a suppressor of abnormal size | adult stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔNZK.UAS, Scer\GAL4^GMR.PU is a suppressor of increased cell death | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

NOT Suppressor of

Statement

Reference

nej^ΔNZK.UAS/Scer\GAL4^unspecified is a non-suppressor of visible | dominant phenotype of so^D

(Kumar et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔNZK.UAS has eye phenotype, enhanceable by lncRNA:Hsrω^EP3037/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔNZK.UAS has eye phenotype, enhanceable by Scer\GAL4^GMR.PF/lncRNA:Hsrω^EP93D

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔNZK.UAS has eye phenotype, enhanceable by rho^unspecified

(Anderson et al., 2005)

Scer\GAL4^GMR.PF, nej^ΔNZK.UAS has eye phenotype, enhanceable by kni^unspecified

(Anderson et al., 2005)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, nej^ΔNZK.UAS has eye phenotype, suppressible by lncRNA:Hsrω^RNAi.Sym.UAS/Scer\GAL4^GMR.PF

(Mallik and Lakhotia, 2010)

Scer\GAL4^GMR.PF, nej^ΔNZK.UAS has eye phenotype, suppressible by gl^unspecified

(Anderson et al., 2005)

Suppressor of

Statement

Reference

nej^ΔNZK.UAS, Scer\GAL4^GMR.PU is a suppressor of eye phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔNZK.UAS, Scer\GAL4^GMR.PU is a suppressor of lens phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

nej^ΔNZK.UAS, Scer\GAL4^GMR.PU is a suppressor of eye disc | third instar larval stage phenotype of Hsap\APP^{Aβ42.UAS.cUa}, Scer\GAL4^GMR.PU

(Cutler et al., 2015)

NOT Suppressor of

Statement

Reference

nej^ΔNZK.UAS/Scer\GAL4^unspecified is a non-suppressor of eye phenotype of so^D

(Kumar et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of P{Sym-UAS-Hsrω} suppresses the Scer\GAL4^GMR.PF, nej^{ΔNZK.Scer\UAS} eye phenotype, and results in near wild type eye morphology.

Co-expression of Hsrω^EP3037 or Hsrω^EP93D enhances the eye damage seen in Scer\GAL4^GMR.PF, nej^{ΔNZK.Scer\UAS} flies.

(Mallik and Lakhotia, 2010)

Expression of nej^{ΔNZK.Scer\UAS} under the control of Scer\GAL4^unspecified does not suppress the no-eye phenotype of so^D/+.

(Kumar et al., 2004)

Xenogenetic Interactions

Statement

Reference

Co-expression of nej^{ΔNZK.Scer\UAS} partially rescues the small eye and lens fusion phenotype seen in adult flies expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU. Co-expression of nej^{ΔNZK.Scer\UAS} partially suppresses the increased cell death observed in third instar eye discs of flies expressing Hsap\APP^{Aβ42.Scer\UAS.cUa} under the control of Scer\GAL4^GMR.PU.

(Cutler et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

nej^{ΔNZK.Scer\UAS}

nej^ΔNZK.UAS

Name Synonyms

Secondary FlyBase IDs

References (4)

Report Sections

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