nej3/+ ; soD/+ adults lack eyes and the nondifferentiated field of tissue that is seen in place of the eye in soD/+ single mutants is replaced by head tissue in the double mutant adults. nej3/+ ; soD/+ eye imaginal discs are small and undergo increased levels of cell death.
When nej3 is added to Myb1 animals at 18oC a dramatic reduction in viability is seen as well as a significantly delayed emergence of surviving animals. When nej3 is added to Myb1/Myb2 animals an even more marked effect is seen on viability and emergence. When nej3 is added to Myb1 animals at 18oC no effect is seen on viability or any delay in development seen. When nej3/+ is added to Myb1 animals at 18oC an enhancement of the multiple wing hair phenotype is seen. The abdomen defects are also enhanced. White differentiated cuticle is present between most segments and along the dorsal midline, indicating fusion defects. Defects in wing cell and nucleus size are also enhanced, nuclei and cells are larger and more variable in size. The mitotic index phenotype is also enhanced in these mutants, though no increase in the rate of centrosomal and chromosomal defects is seen.
Double mutants for sggM11 and nej3 show a worse gut phenotype than do nej3 single mutants. The embryonic epidmeris phenotype is suppressed in double mutants with arm2. Dominantly suppresses the wing margin phenotype of shgScer\UAS.cSa, Scer\GAL4en-e16E.