FlyBase Allele Report: Dmel\shg[UAS.cSa]

General Information

Symbol

Dmel\shg^UAS.cSa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Sanson

FlyBase ID

FBal0056179

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{UAS-shg.CAD}

Also Known As

UAS-DE-cadherin, UAS-DE-cad, UAS-CAD, UAS-CADH, UAS-DECad

Key Links

Transgenic product class

cDNA

(Sanson et al., 1996)

wild_type

(Sanson et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Sanson et al., 1996)

wild_type

(Sanson et al., 1996)

Mutagen

in vitro construct

(Sanson et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-shg.CAD}

Cytology

Description

UASt regulatory sequences drive expression of a full-length shg cDNA.

(Sanson et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

shg

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA

(Shen et al., 2014)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

DOES NOT ameliorate cancer

modeled by bi^UAS.cPa

(Shen et al., 2014)

model of cancer

is NOT ameliorated by bi^UAS.cBa

(Shen et al., 2014)

is NOT ameliorated by bi^UAS.cPa

(Shen et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Bolwig organ, with Scer\GAL4^da.G32

(Dumstrei et al., 2002)

border follicle cell, with Scer\GAL4^slbo.2.6

(Schober et al., 2005)

border follicle cell, with Scer\GAL4^{VP16.nanos.UTR}, Scer\GAL4^VP16.otu, Scer\GAL4^nanos.PG

(Cai et al., 2014)

denticle & embryonic epidermis | somatic clone, with Scer\GAL4^αTub84B.PP

(Sanson et al., 1999)

denticle | ectopic | somatic clone, with Scer\GAL4^αTub84B.PP

(Sanson et al., 1999)

denticle | somatic clone, with Scer\GAL4^αTub84B.PP

(Sanson et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^VP16.arm

(Sanson et al., 1996)

embryonic epidermis | somatic clone, with Scer\GAL4^αTub84B.PP

(Sanson et al., 1999)

embryonic optic lobe primordium, with Scer\GAL4^da.G32

(Dumstrei et al., 2002)

epithelial cell | third instar larval stage, with Scer\GAL4^dpp.PU

(Shen et al., 2014)

fat body/gonad primordium, with Scer\GAL4^{VP16.nanos.UTR}

(Jenkins et al., 2003)

gonadal sheath proper primordium, with Scer\GAL4^{VP16.nanos.UTR}

(Jenkins et al., 2003)

ommatidium, with Scer\GAL4^hs.2sev

(Mirkovic and Mlodzik, 2006)

oocyte | cell non-autonomous | somatic clone, with Scer\GAL4^Act5C.PP

(Becam et al., 2005)

wing, with Scer\GAL4^en-e16E

(Waltzer and Bienz, 1998)

wing blade posterior compartment, with Scer\GAL4^en-e16E

(Barker et al., 2001, Sanson et al., 1996)

wing disc | somatic clone, with Scer\GAL4^Act5C.PP

(Dahmann and Basler, 2000)

wing disc | third instar larval stage, with Scer\GAL4^dpp.PU

(Shen et al., 2014)

wing margin, with Scer\GAL4^en-e16E

(Barker et al., 2001)

Detailed Description

Statement

Reference

Overexpression of shg^Scer\UAS.cSa 6-fold in nurse cells (under the control of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG, and Scer\GAL4^{nos.UTR.T:Hsim\VP16}) results in border cells migrating along a normal trajectory more slowly than normal and exhibiting a hyperpolarized morphology. A total of 86% of protrusions from border cell clusters migrating between nurse cells are directed toward the oocyte (compared to 76% in wild-type).

Overexpression of shg in outer, migratory border cells using Scer\GAL4^slbo.2.6 or in polar cell using Scer\GAL4^upd.PU does not affect border cell migration significantly.

(Cai et al., 2014)

The expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^dpp.PU induces long distance cell migration and scattering, even across the anterior-posterior compartment border, in third instar larval wing discs, as compared to controls.

(Shen et al., 2014)

Expression of shg^Scer\UAS.cSa, under the control of Scer\GAL4^e22c, results in clones that are apically constricted.

Somatic clones overexpressing shg^Scer\UAS.cSa under the control of Scer\GAL4^vg.PM, separate from their neighbours and display smooth clone borders.

(Jaiswal et al., 2006)

Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^hs.2sev in ommatidia at the time of rotation has no effect on eyes when expressed at moderate levels and causes epithelial defects at high levels.

The ommatidial under-rotation of Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs} eyes is suppressed in Scer\GAL4^hs.2sev>shg^{dCR3h.Scer\UAS.T:Avic\GFP-rs}; Scer\GAL4^hs.2sev>shg^Scer\UAS.cSa flies.

Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^hs.2sev increases the number of ommatidial clusters that initiate rotation correctly in shg^k03401 clones.

(Mirkovic and Mlodzik, 2006)

Clones of follicle cells that are expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP can cause mislocalisation of the oocyte, and in these cases the mislocalised oocyte is always in contact with a cell or group of cells expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP.

(Becam et al., 2005)

Expression of shg^Scer\UAS.cSa, under the control of Scer\GAL4^elav-C155, does not suppress the R cell axon extension phenotype of Df(2L)CadN^Δ14 clones.

(Prakash et al., 2005)

Expression of shg^Scer\UAS.cSa in outer border cells, driven by Scer\GAL4^slbo.2.6, delays border cell migration and blocks their detachment from the anterior follicular epithelium.

(Schober et al., 2005)

In shg^Scer\UAS.cSa; Scer\GAL4^{nos.UTR.T:Hsim\VP16} embryos, both the cell bodies and ensheathing cytoplasmic extensions of the somatic germ cell precursors are excluded from the cluster of germ cells.

(Jenkins et al., 2003)

When shg^Scer\UAS.cSa is driven by Scer\GAL4^btl.PS tracheal development appears unaffected.

(Lee et al., 2003)

Cells of the optic placode maintain their epithelial shape but do not invaginate in embryos expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^da.G32. The Bolwig's organ does not separate from the optic lobe, in contrast to wild type.

(Dumstrei et al., 2002)

Expression driven by Scer\GAL4^en-e16E causes notching in the posterior compartment of the wing.

(Barker et al., 2001)

Clones in the wing disc expressing shg^Scer\UAS.cSa under the control of Scer\GAL4^Act5C.PP form smooth borders with their neighbouring cells in both the anterior and posterior compartment. If in contact with each other, clones tend to fuse and interdigitate irrespective of the compartment boundary.

(Dahmann and Basler, 2000)

When expression is driven in clones in the embryonic epidermis by Scer\GAL4^αTub84B.PP, the response of the cells to wg is blocked and ectopic denticles result. When the clones fall within the normal denticle belt, normal denticles are formed.

(Sanson et al., 1999)

When expression is driven by Scer\GAL4^en-e16E gaps in the wing margin occur.

(Waltzer and Bienz, 1998)

Scer\GAL4^{arm.T:Hsim\VP16} mediated expression causes a cuticle phenotype identical to wg^l-17. Scer\GAL4^en-e16E mediated expression causes loss of wing margin in the posterior compartment.

(Sanson et al., 1996)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^dpp.PU, shg^UAS.cSa has abnormal cell migration | third instar larval stage phenotype, non-enhanceable by bi^UAS.cPa/Scer\GAL4^dpp.PU

(Shen et al., 2014)

Suppressor of

Statement

Reference

shg^UAS.cSa/Scer\GAL4^332.3 is a suppressor | partially of majority die during embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^tj.PU is a suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

NOT Suppressor of

Statement

Reference

shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of decreased cell number | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^dpp.PU is a non-suppressor of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^dpp.PU, bi^UAS.cPa

(Shen et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^en-e16E, shg^UAS.cSa has wing margin phenotype, enhanceable by brm[+]/brm²

(Barker et al., 2001)

Scer\GAL4^en-e16E, shg^UAS.cSa has wing blade posterior compartment phenotype, enhanceable by brm[+]/brm²

(Barker et al., 2001)

Scer\GAL4^en-e16E, shg^UAS.cSa has wing margin phenotype, enhanceable by mor²/mor[+]

(Barker et al., 2001)

Scer\GAL4^en-e16E, shg^UAS.cSa has wing blade posterior compartment phenotype, enhanceable by mor²/mor[+]

(Barker et al., 2001)

Scer\GAL4^en-e16E, shg^UAS.cSa has wing margin phenotype, enhanceable by pan[+]/pan²

(Barker et al., 2001)

Scer\GAL4^en-e16E, shg^UAS.cSa has wing blade posterior compartment phenotype, enhanceable by pan[+]/pan²

(Barker et al., 2001)

Scer\GAL4^en-e16E, shg^UAS.cSa has wing phenotype, enhanceable by arm²/arm[+]

(Waltzer and Bienz, 1998)

NOT Enhanced by

Statement

Reference

Scer\GAL4^dpp.PU, shg^UAS.cSa has wing disc | third instar larval stage phenotype, non-enhanceable by bi^UAS.cPa/Scer\GAL4^dpp.PU

(Shen et al., 2014)

Scer\GAL4^dpp.PU, shg^UAS.cSa has epithelial cell | third instar larval stage phenotype, non-enhanceable by bi^UAS.cPa/Scer\GAL4^dpp.PU

(Shen et al., 2014)

Suppressed by

Statement

Reference

Scer\GAL4^en-e16E, shg^UAS.cSa has wing phenotype, suppressible by nej[+]/nej³

(Waltzer and Bienz, 1998)

Suppressor of

Statement

Reference

shg^UAS.cSa/Scer\GAL4^332.3 is a suppressor | partially of embryo | dorsal closure stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

shg^UAS.cSa/Scer\GAL4^332.3 is a suppressor | partially of embryonic/first instar larval cuticle | embryonic stage phenotype of crb^GX24w-/crb^11A22, crb^Y10A

(Flores-Benitez and Knust, 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor | partially of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^upd1.PU is a suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^tj.PU is a suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^tj.PU is a suppressor | partially of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^tj.PU is a suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^unspecified is a suppressor of follicle stem cell | somatic clone phenotype of CycE^WX

(Wang and Kalderon, 2009)

shg^UAS.cSa is a suppressor of secondary pigment cell | increased number phenotype of Csk^RNAi.UAS, Scer\GAL4^GMR.PF

(Vidal et al., 2006)

shg^UAS.cSa is a suppressor of tertiary pigment cell | increased number phenotype of Csk^RNAi.UAS, Scer\GAL4^GMR.PF

(Vidal et al., 2006)

shg^UAS.cSa/Scer\GAL4^slbo.2.6 is a suppressor | partially of border follicle cell phenotype of Scer\GAL4^slbo.2.6, jar^a.UAS

(Geisbrecht and Montell, 2002)

NOT Suppressor of

Statement

Reference

shg^UAS.cSa/Scer\GAL4^upd1.PU is a non-suppressor of male fusome phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of hub cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of male germline stem cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^{VP16.nanos.UTR} is a non-suppressor of cyst progenitor cell | adult stage phenotype of Df(3R)ED6235/jus^iso7.8

(Lim et al., 2015)

shg^UAS.cSa/Scer\GAL4^dpp.PU is a non-suppressor of wing disc | third instar larval stage phenotype of Scer\GAL4^dpp.PU, bi^UAS.cPa

(Shen et al., 2014)

shg^UAS.cSa/Scer\GAL4^dpp.PU is a non-suppressor of epithelial cell | third instar larval stage phenotype of Scer\GAL4^dpp.PU, bi^UAS.cPa

(Shen et al., 2014)

shg^UAS.cSa, Scer\GAL4^Act5C.PI is a non-suppressor of adherens junction | somatic clone phenotype of Rho1^RNAi.1.UAS, Scer\GAL4^Act5C.PI

(Warner and Longmore, 2009)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^332.3 largely suppresses the embryonic lethality and dorsal closure defects in embryos expressing crb^Y10A in a crb^GX24w-/crb^11A22 background, resulting in some adult survival.

(Flores-Benitez and Knust, 2015)

Expression of shg^Scer\UAS.cSa under the control of either Scer\GAL4^upd1.PU or Scer\GAL4^tj.PU fully rescues the decreased cell numbers of hub cells and cyst stem cells, and partially rescues the decreased number of germline stem cells, in the adult testicular niche of sda^iso7.8/Df(3R)ED6235 mutants.

Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^upd1.PU fails to rescue the decrease in germline stem cells with disintegrating fusomes seen in sda^iso7.8/Df(3R)ED6235 mutants.

Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} fails to rescue the germline stem cell, cyst stem cell, and hub cell number phenotypes in testicular niches of sda^iso7.8/Df(3R)ED6235 mutants.

(Lim et al., 2015)

The co-expression of both bi^Scer\UAS.cBa and shg^Scer\UAS.cSa under the control of Scer\GAL4^dpp.PU does not significantly suppress the long distance cell migration and scattering observed in third instar larval wing discs upon the expression of only bi^Scer\UAS.cBa or only shg^Scer\UAS.cSa, under the control of Scer\GAL4^dpp.PU.

(Shen et al., 2014)

Scer\GAL4^unspecified-driven expression of shg^Scer\UAS.cSa suppresses the loss of follicle stem cells in somatic clones of CycE^WX.

(Wang and Kalderon, 2009)

The extra secondary and tertiary pigment cell phenotype seen in the developing retinas of Csk^IR.Scer\UAS; Scer\GAL4^GMR.PF animals at 42 hours after puparium formation is suppressed by shg^Scer\UAS.cSa.

(Vidal et al., 2006)

The inhibition of border cell migration seen in the ovaries of jar^a.Scer\UAS; Scer\GAL4^slbo.2.6 flies is significantly suppressed by the presence of shg^hs.PW under heat shock condition (flies raised at 29^oC and given a 1 hour heat shock at 37^oC 6-10 hours prior to dissection.). Partial suppression is also seen in flies carrying slbo^Scer\UAS.cLa or shg^Scer\UAS.cSa, but not Fak56D^{Scer\UAS.T:Ivir\HA1}.

(Geisbrecht and Montell, 2002)

The wing phenotype caused by shg^Scer\UAS.cSa expressed under the control of Scer\GAL4^en-e16E is exacerbated by heterozygosity for arm² and suppressed by heterozygosity for nej³.

(Waltzer and Bienz, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

shg^UAS.cSa/Scer\GAL4^{FRT.Rnor\Cd2.αTub84B} rescues shg^R69

(Pacquelet and Rorth, 2005)

shg^UAS.cSa/Scer\GAL4^{FRT.Rnor\Cd2.αTub84B} rescues shg^k03401

(Pacquelet and Rorth, 2005)

shg^UAS.cSa/Scer\GAL4^arm.PS rescues shg¹

(Sanson et al., 1996)

shg^UAS.cSa/Scer\GAL4^arm.PS rescues shg²

(Sanson et al., 1996)

Comments

Expression of shg^Scer\UAS.cSa under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.αTub84B} suppresses the follicle cell sorting, loss of epithelial integrity and oocyte mis-positioning phenotypes seen in shg^R69 mutant follicle cell clones.

Expression of shg^Scer\UAS.cSa can suppress the shg^k03401 mutant follicle cell clone phenotype (follicle cell sorting, loss of epithelial integrity, and oocyte positioning).

Expression of shg^Scer\UAS.cSa can suppress the migration defects of shg^k03401 mutant border cell clones.

(Pacquelet and Rorth, 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

shg^Scer\UAS.cSa

shg^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sanson

(Sanson et al., 1996)

Secondary FlyBase IDs

References (33)

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