FlyBase Allele Report: Dmel\pan[2]

General Information

Symbol

Dmel\pan²

Species

D. melanogaster

Name

FlyBase ID

FBal0059982

Feature type

allele

Associated gene

Dmel\pan

Associated Insertion(s)

Carried in Construct

Also Known As

dTCF², Tcf²

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(van de Wetering et al., 1997)

Mutagen

Progenitor genotype

Cytology

Description

Single base deletion (ATT to AT at I106) causing a frameshift.

(van de Wetering et al., 1997)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

4:82,649..82,649 [+]

Comment:

A single base deletion (ATT to AT in I107) causes a frameshift.

(van de Wetering et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Huntington's disease

modeled by Hsap\HTT^Q93.ex1.UAS

(Dupont et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

denticle | ectopic

(Overton et al., 2007)

embryonic/first instar larval cuticle | germline clone | rescuable maternal effect

(Schweizer et al., 2003)

embryonic epidermis | ventral

(McEwen et al., 2000)

segment

(van de Wetering et al., 1997)

ventral denticle belt

(McEwen et al., 2000)

wing margin bristle | cell non-autonomous | ectopic | somatic clone

(Schweizer et al., 2003)

Detailed Description

Statement

Reference

Mutant larval have a "lawn of denticles" cuticle phenotype.

(Overton et al., 2007)

When homozygous somatic clones are induced during the larval second instar, clones and twin spots are of similar size. Earlier induction, during the first instar, causes a reduction in size of mutant clones in the wing-blade primordium. Homozygous somatic clones at the wing margin cause the formation of ectopic wing margin bristles in adjacent wild-type territory. No planar polarity defects are observed in clones on adult wings. Embryos with maternal pan², display segment polarity phenotypes resembling those of zygotic pan² mutants. The alternation of naked cuticle and denticle belts seen in wild-type is replaced by an under-representation of naked cuticle. Embryos with both maternal and zygotic pan², do not exhibit a stronger phenotype than those lacking maternal pan only. These phenotypes can be significantly rescued by paternal pan.

(Schweizer et al., 2003)

Ventral segment polarity defects.

(McEwen et al., 2000)

Regions of naked cuticle are replaced by cuticle with denticles. Head segments are relatively unaffected.

(van de Wetering et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

pan[+]/pan² is a suppressor | partially of short lived phenotype of Hsap\HTT^Q93.ex1.UAS, Scer\GAL4^Eaat1.PR

(Dupont et al., 2012)

pan² is a suppressor of visible phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

Phenotype Manifest In

Suppressed by

Statement

Reference

pan² has denticle | ectopic phenotype, suppressible by SoxN^U6-35/SoxN[+]

(Overton et al., 2007)

pan² has denticle | ectopic phenotype, suppressible by SoxN^U6-35/SoxN^U6-35

(Overton et al., 2007)

pan² has ventral denticle belt phenotype, suppressible by puc^A251.1F3

(McEwen et al., 2000)

pan² has embryonic epidermis | ventral phenotype, suppressible by puc^A251.1F3

(McEwen et al., 2000)

Enhancer of

Statement

Reference

pan[+]/pan² is an enhancer of embryonic/first instar larval cuticle phenotype of SoxN^GA1192

(Chao et al., 2007)

pan[+]/pan² is an enhancer of ventral denticle belt phenotype of SoxN^GA1192

(Chao et al., 2007)

pan[+]/pan² is an enhancer of embryonic/first instar larval cuticle phenotype of SoxN^NC14

(Chao et al., 2007)

pan[+]/pan² is an enhancer of ventral denticle belt phenotype of SoxN^NC14

(Chao et al., 2007)

pan[+]/pan² is an enhancer of rhabdomere | conditional phenotype of Hsap\HTT^GMR.Q120

(Berger et al., 2005)

pan[+]/pan² is an enhancer of wing margin phenotype of Scer\GAL4^en-e16E, shg^UAS.cSa

(Barker et al., 2001)

pan[+]/pan² is an enhancer of wing blade posterior compartment phenotype of Scer\GAL4^en-e16E, shg^UAS.cSa

(Barker et al., 2001)

NOT Enhancer of

Statement

Reference

pan², Scer\GAL4^30A is a non-enhancer of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Suppressor of

Statement

Reference

pan² is a suppressor of embryonic/first instar larval cuticle phenotype of wg^l-17

(Bejsovec and Chao, 2012)

pan² is a suppressor of denticle phenotype of wg^l-17

(Bejsovec and Chao, 2012)

pan² is a suppressor of macrochaeta & wing | ectopic | somatic clone phenotype of sgg³²

(Schweizer et al., 2003)

pan² is a suppressor of eye | anterior phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

pan² is a suppressor of eye phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

pan² is a suppressor of ommatidium phenotype of Hsap\MAPT^GMR.Ex.PJ

(Jackson et al., 2002)

pan[+]/pan² is a suppressor of eye phenotype of arm^S56F.GMR

(Barker et al., 2001, Freeman and Bienz, 2001)

pan² is a suppressor of embryonic epidermis phenotype of arm⁴

(Cavallo et al., 1998)

pan² is a suppressor of ventral denticle belt phenotype of arm⁴

(Cavallo et al., 1998)

pan² is a suppressor of embryonic epidermis phenotype of arm²

(Cavallo et al., 1998)

pan² is a suppressor of ventral denticle belt phenotype of arm²

(Cavallo et al., 1998)

pan² is a suppressor of embryonic epidermis phenotype of arm³

(Cavallo et al., 1998)

pan² is a suppressor of ventral denticle belt phenotype of arm³

(Cavallo et al., 1998)

NOT Suppressor of

Statement

Reference

pan², Scer\GAL4^30A is a non-suppressor of wing phenotype of Pka-C1^UAS.cKa, Scer\GAL4^30A

(Kiger et al., 2001)

Other

Statement

Reference

ck^KT9, pan², wg^l-17 has denticle phenotype

(Bejsovec and Chao, 2012)

pan², puc^A251.1F3 has embryonic epidermis | dorsal phenotype

(McEwen et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

pan² partially suppresses the wg^l-17 denticle phenotype: denticle diversity is restored but not naked cuticle.

In wg^l-17 ck^KT9 ; pan² triple mutants, smaller anterior denticles can have sharp points, while larger posterior denticles remain rounded and some appear fragmented.

(Bejsovec and Chao, 2012)

Approximately 56.7% of pan²/+ ; SoxN^NC14/+ double mutants appear phenotypically wild-type. Approximately 18.6% of mutants exhibit a 'lawn of denticles' phenotype, typical of arm mutants. The SoxN^NC14 mutation disrupts the dorsal pattern and reduces the size of the embryos. A SoxN-like phenotype is observed in approximately 18.9% of the double mutants. Approximately 5.8% of the double mutants exhibit a severe excess-naked-cuticle SoxN-like phenotype. The double mutants exhibit more extensive naked cuticle than SoxN^NC14 single mutants.

Approximately 55.9% of pan²/+ ; SoxN^GA1192/+ double mutants appear phenotypically wild-type. Approximately 19.2% of mutants exhibit a 'lawn of denticles' phenotype, typical of arm mutants. The SoxN^GA1192 mutation disrupts the dorsal pattern and reduces the size of the embryos. A SoxN-like phenotype is observed in approximately 17.7% of the double mutants. Approximately 7.2% of the double mutants exhibit a severe excess-naked-cuticle SoxN-like phenotype. The double mutants exhibit more extensive naked cuticle than SoxN^GA1192 single mutants.

(Chao et al., 2007)

SoxN^U6-35/+ partially suppresses the formation of ectopic denticles seen in pan² mutant larvae, while pan² ; SoxN^U6-35 double mutants have the same cuticle phenotype as SoxN^U6-35 single mutants.

(Overton et al., 2007)

Somatic clones homozygous for both sgg³² and pan² show a suppression of the ectopic bristle phenotype seen in sgg³² clones alone.

(Schweizer et al., 2003)

pan²,puc^A251.1F3 double mutants show a novel loss of dorsal cuticle phenotype.

(McEwen et al., 2000)

Xenogenetic Interactions

Statement

Reference

pan²/+ significantly partially suppresses the decreased lifespan seen in flies with expression of Hsap\HTT^{Q93.ex1p.Scer\UAS} driven by Scer\GAL4^Eaat1.PR.

(Dupont et al., 2012)

Heterozygosity for pan² enhances the loss of rhabdomeres seen in Hsap\HTT^GMR.Q120 flies.

(Berger et al., 2005)

Complementation and Rescue Data

Fails to complement

pan^Δ31

(Kronhamn and Rasmuson-Lestander, 1999)

pan^Δ3M

(Kronhamn and Rasmuson-Lestander, 1999)

pan^Δ9M

(Kronhamn and Rasmuson-Lestander, 1999)

pan^ciD

(Kronhamn and Rasmuson-Lestander, 1999)

pan^IA5

(Kronhamn and Rasmuson-Lestander, 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4759

w¹¹¹⁸; pan²/P{ActGFP}unc-13^GJ

Kyoto

107860

pan²/Dp(2;4)ey^D, Ablp^eyD: ey^D

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

TCF²

(Ravindranath and Cadigan, 2014)

Tcf²

(Bejsovec and Chao, 2012, Chao et al., 2007)

dTC3²

(Barker et al., 2001)

dTCF²

(Berger et al., 2005, Jackson et al., 2002, Streit et al., 2002, Freeman and Bienz, 2001, Llimargas, 2000, McEwen et al., 2000, Roose and Clevers, 1999, Cavallo et al., 1998, Yu et al., 1998, van de Wetering et al., 1997)

dTcf²

(Yang et al., 2000)

pan²

(Clarembaux-Badell et al., 2022, Xie et al., 2017, Dupont et al., 2012, Overton et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (25)

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