FlyBase Allele Report: Dmel\Src42A[UAS.cPa]

General Information

Symbol

Dmel\Src42A^UAS.cPa

Species

D. melanogaster

Name

FlyBase ID

FBal0175798

Feature type

allele

Associated gene

Dmel\Src42A

Associated Insertion(s)

Carried in Construct

P{UAS-Src42A.P}

Also Known As

UAS-Src42A, UAS-Src42

Key Links

Transgenic product class

cDNA

(Pedraza et al., 2004)

wild_type

(Pedraza et al., 2004)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Pedraza et al., 2004)

wild_type

(Pedraza et al., 2004)

Mutagen

in vitro construct

(Pedraza et al., 2004)

Progenitor genotype

Carried in construct

P{UAS-Src42A.P}

Cytology

Description

UASt sequences drive expression of a Src42A cDNA.

(Pedraza et al., 2004)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Src42A

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Increased expression of Src42A^UAS.cPa under the control of Scer\GAL4^GMR.PF at 25[o]C (but not 18[o]C) results in a significant proportion of shorter CadN-immunopositive adherens junctions between photoreceptor precursor cells R3 and R4 in row 5 ommatidia of wandering third instar larvae compared to controls.

(Pütz, 2019)

Expression of Src42A^Scer\UAS.cPa under the control of Scer\GAL4^btl.PS results in over-elongated and convoluted dorsal trunks.

(Förster and Luschnig, 2012)

When Src42A^Scer\UAS.cPa is driven by Scer\GAL4^GMR.PF, a range of phenotypes is seen in the eye in an insertion, dose dependent and temperature sensitive manner. These range through a complete loss of the eye, through small rough eyes, rough eyes with ectopic bristles to no phenotype at all. When the developing eye disc is examined, ectopic proliferation of uncommitted cells (which usually arrest in G₁/G₀) in the disc is seen. Mutant eye discs also exhibit increased cell death.

(Pedraza et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has increased occurrence of cell division | larval stage phenotype, enhanceable by Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

NOT Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has increased cell death phenotype, non-enhanceable by Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has abnormal neuroanatomy | wandering third instar larval stage phenotype, suppressible by mbt^+tPa/mbt[+]

(Pütz, 2019)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has abnormal cell adhesion | wandering third instar larval stage phenotype, suppressible by mbt^+tPa/mbt[+]

(Pütz, 2019)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has abnormal size | wandering third instar larval stage phenotype, suppressible by mbt^+tPa/mbt[+]

(Pütz, 2019)

Scer\GAL4^NP7397, Src42A^UAS.cPa has increased occurrence of cell division | adult stage phenotype, suppressible by Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has increased cell death phenotype, suppressible by Csk^UAS.cPa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has increased occurrence of cell division | larval stage phenotype, suppressible by Csk^UAS.cPa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src42A^UAS.cPa has increased occurrence of cell division | adult stage phenotype, non-suppressible by Egfr^f24

(Kohlmaier et al., 2015)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has increased cell death phenotype, non-suppressible by Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Other

Statement

Reference

GluRIIA^SP16, Scer\GAL4^C57, Src42A^UAS.cPa has abnormal neurophysiology | third instar larval stage phenotype

(Spring et al., 2016)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has eye phenotype, enhanceable by Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has eye disc | larval stage phenotype, enhanceable by Hsap\CDKN1A^UAS.cHa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has eye phenotype, enhanceable by Csk^j1D8, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has photoreceptor R3 precursor cell | wandering third instar larval stage phenotype, suppressible by mbt^+tPa/mbt[+]

(Pütz, 2019)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has photoreceptor R4 precursor cell | wandering third instar larval stage phenotype, suppressible by mbt^+tPa/mbt[+]

(Pütz, 2019)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has adherens junction | wandering third instar larval stage phenotype, suppressible by mbt^+tPa/mbt[+]

(Pütz, 2019)

Scer\GAL4^NP7397, Src42A^UAS.cPa has adult midgut phenotype, suppressible by Egfr^RNAi.UAS.cUa, Scer\GAL4^NP7397

(Kohlmaier et al., 2015)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has eye phenotype, suppressible by Csk^UAS.cPa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

Scer\GAL4^GMR.PF, Src42A^UAS.cPa has eye disc | larval stage phenotype, suppressible by Csk^UAS.cPa, Scer\GAL4^GMR.PF

(Pedraza et al., 2004)

NOT suppressed by

Statement

Reference

Scer\GAL4^NP7397, Src42A^UAS.cPa has adult midgut phenotype, non-suppressible by Egfr^f24

(Kohlmaier et al., 2015)

Other

Statement

Reference

GluRIIA^SP16, Scer\GAL4^C57, Src42A^UAS.cPa has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Spring et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae with expression of Src42A^Scer\UAS.cPa driven in muscles by Scer\GAL4^C57 (at 18[o]C) show significant decreases in mEPSP (decreased quantal size) but do not show significant compensatory homeostatic increases in quantal content (as seen in GluRIIA^SP16/GluRIIA^SP16 mutants alone) at the NMJ.

(Spring et al., 2016)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

82157