FlyBase Allele Report: Dmel\fru[GAL4.P1.D]

General Information

Symbol

Dmel\fru^GAL4.P1.D

Species

D. melanogaster

Name

FlyBase ID

FBal0179808

Feature type

allele

Associated gene

Dmel\fru

Associated Insertion(s)

TI{GAL4}fru^GAL4.P1.D

Carried in Construct

Also Known As

fru-GAL4, fru^GAL4, FruP1-GAL4, fru^GAL4.P1

Key Links

Allele class

Mutagen

cre recombinase

Nature of the Allele

Allele class

Mutagen

cre recombinase

(Stockinger et al., 2005)

Progenitor genotype

TI{GAL4}fru^GAL4.GFP

(Stockinger et al., 2005)

Associated Insertion(s)

TI{GAL4}fru^GAL4.P1.D

Cytology

Description

The loxP cassette containing the Avic\GFP^GMR.PS marker has been excised. This results in a fru locus in which the endogenous fru P1 promoter drives expression of GAL4 instead of the fru[M] isoform. A transcriptional stop signal and a single loxP site remain downstream of the GAL4 open reading frame.

(Stockinger et al., 2005)

Allele components

Component

Use(s)

Inserted element

TI{GAL4}

promoter trap

Encoded product / tool

GAL4

binary expression system - driver

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

gustatory receptor neuron

(Mellert et al., 2012)

Additional Information

Statement

Reference

Marker for

Reflects expression of

fru

(Mellert et al., 2012)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | male

(Stockinger et al., 2005)

abnormal courtship behavior | male (with fru³)

(Stockinger et al., 2005)

abnormal courtship behavior | male (with fru⁴)

(Stockinger et al., 2005)

abnormal courtship behavior | male (with fru^AJ96u3)

(Stockinger et al., 2005)

abnormal courtship behavior | male limited

(Ramdya et al., 2012)

abnormal size | adult stage | male (with fru^ΔC)

(Zhang et al., 2020)

decreased size | adult stage | male

(Zhang et al., 2020)

decreased size | adult stage | male (with fru^ΔA)

(Zhang et al., 2020)

semi-fertile | male

(Stockinger et al., 2005)

semi-fertile | male (with fru³)

(Stockinger et al., 2005)

semi-fertile | male (with fru⁴)

(Stockinger et al., 2005)

semi-fertile | male (with fru^AJ96u3)

(Stockinger et al., 2005)

Phenotype Manifest In

antennal lobe glomerulus DA1 | adult stage | male

(Zhang et al., 2020)

antennal lobe glomerulus DA1 | adult stage | male (with fru^ΔA)

(Zhang et al., 2020)

antennal lobe glomerulus DA1 | adult stage | male (with fru^ΔC)

(Zhang et al., 2020)

antennal lobe glomerulus VA1v | adult stage | male

(Zhang et al., 2020)

antennal lobe glomerulus VA1v | adult stage | male (with fru^ΔA)

(Zhang et al., 2020)

antennal lobe glomerulus VA1v | adult stage | male (with fru^ΔC)

(Zhang et al., 2020)

antennal lobe glomerulus VL2a | adult stage | male

(Zhang et al., 2020)

antennal lobe glomerulus VL2a | adult stage | male (with fru^ΔA)

(Zhang et al., 2020)

Detailed Description

Statement

Reference

fru^GAL4.P1.D homozygous males court other males, sometimes forming chains that incorporate both wild-type and fru^GAL4.P1.D/fru^GAL4.P1.D animals.

fru^GAL4.P1.D homozygous males court wild type and fru^GAL4.P1.D/fru^GAL4.P1.D flies with similar intensity.

(Ramdya et al., 2012)

The male-female courtship index of fru^GAL4/fru^GAL4 males is not significantly reduced compared to wild-type, but male fertility drops over 50%. The male-female courtship index of males carrying fru^GAL4 in trans to fru³, fru³, or fru^AJ96u3 is significantly reduced, and fertility is reduced to around 20% of wild-type. Males of all these males score highly in assays for male-male courtship assays (courtship index with single wild-type males and chaining with males of their own genotype).

(Stockinger et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Photoactivation of fru-expressing neurons in headless females expressing Rnor\P2rx2^Scer\UAS.cLa under the control of Scer\GAL4^fru-GAL4 in a fru^M/fru^GAL4 background produces courtship songs with acoustic characteristics similar to those seen in headless males expressing Rnor\P2rx2^Scer\UAS.cLa under the control of Scer\GAL4^fru-GAL4 in a fru[+] background. Recordings of these courtship songs substitute fully for the songs of courting males in an assay where the song is played back to fly pairs consisting of a wingless male (which cannot produce a courtship song) and a virgin female).

(Clyne and Miesenbock, 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

66696

TI{GAL4}fru^GAL4.P1.D/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

fru^GAL4.P1.D

fru^GAL4.P1

(Sethi et al., 2019)

fru^GAL4

(Chen et al., 2021, Chowdhury et al., 2020, Wu et al., 2019, Mellert et al., 2012, Nojima et al., 2010, Clyne and Miesenbock, 2008, Stockinger et al., 2005)

fru^Gal4

(Zhou et al., 2021, Rezával et al., 2014, Kohl et al., 2013, Ramdya et al., 2012)

fru^P1-GAL4

(Garner et al., 2018)

fru^P1.GAL4

(Sethi et al., 2019)

Name Synonyms

fruitlessGal4

(Rezával et al., 2014)

Secondary FlyBase IDs

References (21)

Report Sections

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