FlyBase Insertion Report: Dmel\TI{GAL4}fru[GAL4.P1.D]

General Information

Symbol

Dmel\TI{GAL4}fru^GAL4.P1.D

Species

D. melanogaster

Name

FlyBase ID

FBti0168666

Feature type

insertion_site

Also Known As

fru-GAL4, Fru^Gal4, FruP1-GAL4

Description

Inserted Element

TI{GAL4}

LINE ID

Affected gene(s)

fru

Viability / fertility

viable

Associated allele(s)

fru^GAL4.P1.D, Scer\GAL4^fru.P1.D

Stock availability

1 publicly available

Genomic Location

Chromosomal Location

3R ( 91A6-91B3 )

Sequence Location

Target / Docking site

Member of Large Scale Dataset(s)

Dataset

Description

Detailed Mapping Data

Chromosome (arm)

Sequence Location

Orientation

Cytological location (computed by FlyBase)

91A6-91B3 (near gene of known cytology)

Cytological location (reported)

Insertion into Natural transposon

Comments concerning location

Sequence Data

Flanking sequence

Inserted Element

Construct

TI{GAL4}

(Stockinger et al., 2005)

Size (Kb)

Component Allele(s)

Molecular map

Affected Gene(s)

Insertion may affect gene

fru

(Chen et al., 2021, Zhou et al., 2021, Chowdhury et al., 2020, Zhang et al., 2020, Massey et al., 2019, Sethi et al., 2019, Wu et al., 2019, Kallman et al., 2015, Kohl et al., 2013, Mellert et al., 2012, Nojima et al., 2010, Stockinger et al., 2005)

Variant Molecular Consequences

Phenotypic Data

Associated Allele

Phenotypic Data

fru^GAL4.P1.D

Phenotypic Class Phenotype Manifest in Detailed Description

Scer\GAL4^fru.P1.D

Phenotypic Class Phenotype Manifest in Detailed Description

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

adult stage

adult fruitless pMP-b (female) neuron

Comment: referred to as DN1

(Ren et al., 2016, Ruta et al., 2010)

adult fruitless aSP-k (female) neuron

Comment: referred to as LC1

(Ruta et al., 2010)

adult fruitless aSP-h (female) neuron

Comment: referred to as DC2

(Ruta et al., 2010)

adult fruitless aSP-f (female) neuron

Comment: referred to as DC1

(Ruta et al., 2010)

adult fruitless aIP-e (female) neuron

Comment: referred to as LC2

(Ruta et al., 2010)

adult fruitless pSP-f neuron

(Cachero et al., 2010)

adult fruitless pSP-e neuron

(Cachero et al., 2010)

adult fruitless pSP-d neuron

(Cachero et al., 2010)

adult fruitless pSP-c neuron

(Cachero et al., 2010)

adult fruitless pSP-b (female) neuron

(Cachero et al., 2010)

adult fruitless pSP-a neuron

(Cachero et al., 2010)

adult fruitless pSG-e neuron

(Cachero et al., 2010)

adult fruitless pSG-d neuron

(Cachero et al., 2010)

adult fruitless pSG-c neuron

(Cachero et al., 2010)

adult fruitless pSG-b neuron

(Cachero et al., 2010)

adult fruitless pSG-a neuron

(Cachero et al., 2010)

adult fruitless pMP-g neuron

(Cachero et al., 2010)

adult fruitless pMP-f (female) neuron

(Cachero et al., 2010)

adult fruitless pMP-e (female) neuron

(Cachero et al., 2010)

adult fruitless pMP-d neuron

(Cachero et al., 2010)

adult fruitless pMP-c (female) neuron

(Cachero et al., 2010)

adult fruitless pMP-b (female) neuron

(Cachero et al., 2010)

adult fruitless pMP-a (female) neuron

(Cachero et al., 2010)

adult fruitless pIP-j neuron

(Cachero et al., 2010)

adult fruitless pIP-i neuron

(Cachero et al., 2010)

descending neuron of the posterior brain DNp36 (female)

(Cachero et al., 2010)

adult fruitless pIP-g neuron

(Cachero et al., 2010)

adult fruitless pIP-f neuron

(Cachero et al., 2010)

adult fruitless pIP-e (female) neuron

(Cachero et al., 2010)

adult fruitless pIP-d neuron

(Cachero et al., 2010)

adult fruitless pIP-c neuron

(Cachero et al., 2010)

adult fruitless pIP-b neuron

(Cachero et al., 2010)

adult fruitless pIP-a (female) neuron

(Cachero et al., 2010)

adult fruitless M neuron

(Cachero et al., 2010)

adult fruitless Lo neuron

(Cachero et al., 2010)

adult fruitless aSP-m neuron

(Cachero et al., 2010)

adult fruitless aSP-l neuron

(Cachero et al., 2010)

adult fruitless aSP-k (female) neuron

(Cachero et al., 2010)

adult fruitless aSP-j neuron

(Cachero et al., 2010)

adult fruitless aSP-i (female) neuron

(Cachero et al., 2010)

adult fruitless aSP-h (female) neuron

(Cachero et al., 2010)

adult fruitless aSP-g (female) neuron

(Cachero et al., 2010)

adult fruitless aSP-f (female) neuron

(Cachero et al., 2010)

adult fruitless aSP-e neuron

(Cachero et al., 2010)

adult fruitless aSP-d neuron

(Cachero et al., 2010)

adult fruitless aSP-c neuron

(Cachero et al., 2010)

adult fruitless aSP-b (female) neuron

(Cachero et al., 2010)

adult fruitless aSP-a (female) neuron

(Cachero et al., 2010)

adult fruitless aSG-a neuron

(Cachero et al., 2010)

adult fruitless aIP-h neuron

(Cachero et al., 2010)

adult fruitless aIP-g (female) neuron

(Cachero et al., 2010)

adult fruitless aIP-f neuron

(Cachero et al., 2010)

adult fruitless aIP-e (female) neuron

(Cachero et al., 2010)

adult fruitless aIP-d (female) neuron

(Cachero et al., 2010)

adult fruitless aIP-c (female) neuron

(Cachero et al., 2010)

adult fruitless aIP-b (female) neuron

(Cachero et al., 2010)

adult fruitless aIP-a neuron

(Cachero et al., 2010)

adult fruitless aDT-h neuron

(Cachero et al., 2010)

adult fruitless aDT-g neuron

(Cachero et al., 2010)

adult fruitless aDT-f neuron

(Cachero et al., 2010)

adult fruitless aDT-e (female) neuron

(Cachero et al., 2010)

adult fruitless aDT-d (female) neuron

(Cachero et al., 2010)

adult fruitless aDT-c neuron

(Cachero et al., 2010)

adult fruitless aDT-b (female) neuron

(Cachero et al., 2010)

adult fruitless aDT-a neuron

(Cachero et al., 2010)

neuron | subset of leg

(Yang et al., 2009)

neuron | subset of antenna

(Yang et al., 2009)

neuron | subset of ejaculatory duct

Comment: 2 neurons

(Yang et al., 2009)

neuron | subset of uterus

(Yang et al., 2009)

neuron | subset of lateral oviduct

(Yang et al., 2009)

antennal lobe glomerulus VA1v

(Grosjean et al., 2011)

neuron | subset

(Al-Anzi et al., 2009)

adult brain | restricted

(Al-Anzi et al., 2009)

adult ventral nerve cord | restricted

(Al-Anzi et al., 2009)

neuron of metathoracic neuromere | restricted

(Al-Anzi et al., 2009)

gnathal ganglion | restricted

(Al-Anzi et al., 2009)

adult mushroom body | restricted

(Al-Anzi et al., 2009)

adult olfactory receptor neuron Ir84a

(Grosjean et al., 2011)

antennal lobe glomerulus VL2a

(Grosjean et al., 2011)

adult olfactory receptor neuron Or67d

(Grosjean et al., 2011)

antennal lobe glomerulus DA1

(Grosjean et al., 2011)

adult olfactory receptor neuron Or47b

(Grosjean et al., 2011)

adult fruitless pMP-a (male) neuron

(Ren et al., 2016)

adult fruitless pMP-a (female) neuron

(Ren et al., 2016)

adult fruitless pMP-b (male) neuron

(Ren et al., 2016)

adult fruitless pMP-c (male) neuron | subset of adult DM3 lineage clone

(Ren et al., 2016)

adult fruitless pMP-c (female) neuron | subset of adult DM3 lineage clone

(Ren et al., 2016)

adult fruitless P1 (male) neuron | subset of adult DM4 lineage clone

(Ren et al., 2016)

adult fruitless pMP-e (female) neuron | subset of adult DM4 lineage clone

(Ren et al., 2016)

adult fruitless pIP-g neuron

(Ren et al., 2016)

adult fruitless pIP-j neuron | subset of adult DM5 lineage clone

(Ren et al., 2016)

adult fruitless pMP-f (male) neuron

(Ren et al., 2016)

adult fruitless pMP-f (female) neuron

(Ren et al., 2016)

descending neuron of the posterior brain DNp36 (male) | subset of adult DM6 lineage clone

(Ren et al., 2016)

descending neuron of the posterior brain DNp36 (female) | subset of adult DM6 lineage clone

(Ren et al., 2016)

adult fruitless pIP-e (male) neuron

(Ren et al., 2016)

adult fruitless pIP-e (female) neuron

(Ren et al., 2016)

adult fruitless pIP-a (male) neuron | subset of adult DL2 lineage clone

(Ren et al., 2016)

adult fruitless pIP-a (female) neuron | subset of adult DL2 lineage clone

(Ren et al., 2016)

adult dMP2 Ilp7 neuron

(Castellanos et al., 2013)

ps1 motor neuron

(Shirangi et al., 2013)

wing axillary muscle hg1 motor neuron

(Shirangi et al., 2013)

dorsal tp motor neuron

(Shirangi et al., 2013)

ventral tp motor neuron

(Shirangi et al., 2013)

flight muscle motor neuron

(Shirangi et al., 2013)

adult Drosulfakinin MP1 neuron | subset

Comment: A FLP/FRT strategy shows TI{GAL4}fru^GAL4.P1.D drives expression in adult ingestion neuron 1 when combined with P{Dsk-FLP.W}.

(Wu et al., 2019)

adult Drosulfakinin MP3 neuron | subset

Comment: A FLP/FRT strategy shows TI{GAL4}fru^GAL4.P1.D drives expression in adult ingestion neuron 1 when combined with P{Dsk-FLP.W}.

(Wu et al., 2019)

adult insulin secreting cell of pars intercerebralis | subset

Comment: A FLP/FRT strategy shows TI{GAL4}fru^GAL4.P1.D drives expression in adult ingestion neuron 1 when combined with P{Dsk-FLP.W}.

(Wu et al., 2019)

gustatory receptor neuron

(Mellert et al., 2012)

adult stage | female

fruitless expressing neuron | subset of uterus

(Häsemeyer et al., 2009)

adult sex peptide sensory neuron

(Häsemeyer et al., 2009)

adult sex peptide sensory neuron of uterus

(Häsemeyer et al., 2009)

Additional Information

Statement

Reference

Drives expression in most fruitless expressing neurons in the adult, including neurons of the male and female reproductive systems, and a small subset of olfactory receptor neurons.

(FlyBase Curators, 2017)

Expression driven by ScerGAL4^fru.P1.D was restricted via a transgenic cell class-lineage intersection (CLIn) system to the following subset of sexually dimorphic fruitless neurons of specific Type II neuroblast lineages using KlacKDR^stg14; 27-39 male and 15-27 female pMP-a neurons - part of the DM1 lineage, 19-27 male and 7-11 female pMP-b neurons - part of the DM2 lineage, 3 male and 1-3 female pMP-c neurons - part of the DM3 lineage, 46-50 male and 4-10 female pMP-e neurons - part of the DM4 lineage, 10-14 pIP-g neurons - part of the DM5 lineage, 2 pIP-j neurons - part of the DM5 lineage, 24-26 male and 12-24 female pMP-f neurons - part of the DM6 lineage, a single pIP-h neuron - part of the DM6 lineage, 20-34 male and 16-24 female pIP-e neurons - part of the DL1 lineage, and 17-27 male and 16-24 female pIP-a neurons - part of the DL2 lineage. All of these neurons show sexually dimorphic differences in their projections. In total, ~19% (~396/2083) male and ~8.3% (~164/1980) female ScerGAL4^fru.P1.D neurons intersect with KlacKDR^stg14.

(Ren et al., 2016)

ScerGAL4^fru.P1.D drives expression in Ilp7 positive postembryonic neurons.

(Castellanos et al., 2013)

ScerGAL4^fru-GAL4 labels the olfactory receptor neurons Ir84a, Or67d and Or47b. It is expressed in the antennal lobe glomeruli VL2 anterior compartment, DA1 and VA1 ventral compartment.

(Grosjean et al., 2011)

ScerGAL4^fru.P1.D drives expression in neurons of the female and male gonads. ScerGAL4^fru.P1.D labels 8 neurons on the female lateral oviduct and uterus and 2 neurons in the male ejaculatory duct. All these neurons co-express AvicGFP^EGFP.ppk.1.

(Yang et al., 2009)

ScerGAL4^fru.P1.D drives expression in 2-3 AvicGFP^EGFP.ppk.1 positive fruitless expressing neurons either side of the uterus.

(Häsemeyer et al., 2009)

Marker for

Reflects expression of

fru

(Ren et al., 2016, Mellert et al., 2012, Grosjean et al., 2011, Cachero et al., 2010, Ruta et al., 2010, Al-Anzi et al., 2009, Yang et al., 2009)

Data on Genetic Line

Line ID

Origin as a multiple insertion line

Progenitor(s) within the Genome

Related Aberration or Balancer

Aberration

Balancer

Stocks (1)

Bloomington

66696

TI{GAL4}fru^GAL4.P1.D/TM3, Sb¹

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (15)

Reported As

Symbol Synonym

Fru-GAL4

(Watanabe et al., 2024, Al-Anzi and Zinn, 2018)

Fru-Gal4

(Thornquist et al., 2020, Zhang et al., 2019, Sutcliffe et al., 2017)

Fru^Gal4

(Clowney et al., 2015)

FruP1-GAL4

(Brovkina et al., 2021)

TI{GAL4}fru^GAL4.P1.D

(Chowdhury et al., 2020, FlyBase, 1992-)

fru-GAL4

(Miao et al., 2025, Shen et al., 2023, Ribeiro et al., 2022, Jois et al., 2018, Shih et al., 2015, Inagaki et al., 2014)

fru-Gal4

(Shiozaki et al., 2024, Jois et al., 2022, Sapiro et al., 2019, Trinh et al., 2019, Chan et al., 2015, Kallman et al., 2015, Garaulet et al., 2014)

fru-P1-GAL4

(Sanders and Arbeitman, 2008)

fru-gal4

(Iftikhar et al., 2019, Sellami and Veenstra, 2015)

fru^GAL4.P1

(Zhang et al., 2020)

fru^GAL4

(Watanabe et al., 2017, Ren et al., 2016, Yilmazer et al., 2016)

fru^Gal4

(Seeholzer et al., 2018)

fru^P1-GAL4

(Grmai et al., 2024)

fruP1-GAL4

(Zhang et al., 2025)

fruP1-Gal4

(Hérault et al., 2024, Chen et al., 2022)

Name Synonyms

Secondary FlyBase IDs

References (50)

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