FlyBase Allele Report: Dmel\ctp[exc39]

General Information

Symbol

Dmel\ctp^exc39

Species

D. melanogaster

Name

FlyBase ID

FBal0180289

Feature type

allele

Associated gene

Dmel\ctp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Ghosh-Roy et al., 2005, Ghosh-Roy et al., 2004)

Mutagen

Delta2-3 transposase

(Ghosh-Roy et al., 2004)

Progenitor genotype

ctp^ins1

(Ghosh-Roy et al., 2004)

Cytology

Description

Imprecise excision of P{lacW}.

(Ghosh-Roy et al., 2004)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

male semi-sterile

(Ghosh-Roy et al., 2004)

male semi-sterile (with Df(3L)fz-GF3b)

(Ghosh-Roy et al., 2004)

partially lethal - majority live (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

viable

(Ghosh-Roy et al., 2004)

visible (with ctp^exc6)

(Ghosh-Roy et al., 2004)

visible (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

Phenotype Manifest In

actin filament & spermatid

(Ghosh-Roy et al., 2005)

cystic bulge

(Ghosh-Roy et al., 2005)

macrochaeta (with ctp^exc6)

(Ghosh-Roy et al., 2004)

macrochaeta (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

nucleus & spermatid

(Ghosh-Roy et al., 2005)

spermatid

(Ghosh-Roy et al., 2004)

spermatid cyst

(Ghosh-Roy et al., 2004)

wing (with ctp^exc6)

(Ghosh-Roy et al., 2004)

wing (with Df(1)bi-DL1)

(Ghosh-Roy et al., 2004)

Detailed Description

Statement

Reference

The number of nuclear bundles and individualization complexes is significantly decreased in the testis of ctp^exc39/Y mutants compared to controls. The F-actin is decreased in the investment cones during ctp^exc39 sperm individualization compared to wild-type controls.

(Ghosh-Roy et al., 2005)

Weak allele. ctp^exc6/ctp^exc39 and ctp^exc39/Df(1)bi-DL1 transheterozygotes exhibit thin bristles and erect wings. The seminal vesicles of ctp^exc39 hemizygous males contain lower numbers of motile sperm than wild type. In the testes of 2 day-old ctp^exc39 adult males, spermatids are abnormally knotted in the middle and coiled at their distal ends inside the cysts. The average length of cysts is shortened in ctp^exc39 testes, as the peak of the elongation cone (EC) distribution profile is shifted to the basal end. The total number of ECs per testis is reduced in ctp^exc39 mutants that carry one copy of Df(3L)GN24; some of these ECs are deformed and the average cyst length is shorter than in ctp^exc39 single mutants. In ctp^exc39/Df(3L)fz-GF3b mutants, there are almost no detectable ECs.

(Ghosh-Roy et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ctp^exc39 has male semi-sterile phenotype, enhanceable by Df(3L)fz-GF3b/+

(Ghosh-Roy et al., 2004)

ctp^exc39 has male semi-sterile phenotype, enhanceable by DCTN1-p150^Gl-1/DCTN1-p150[+]

(Ghosh-Roy et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

ctp^exc39 has cystic bulge phenotype, enhanceable by Df(3L)GN24/+

(Ghosh-Roy et al., 2005)

ctp^exc39 has nucleus & spermatid phenotype, enhanceable by Df(3L)GN24/+

(Ghosh-Roy et al., 2005)

ctp^exc39 has cystic bulge phenotype, enhanceable by DCTN1-p150^Gl-1/DCTN1-p150[+]

(Ghosh-Roy et al., 2005)

ctp^exc39 has nucleus & spermatid phenotype, enhanceable by DCTN1-p150^Gl-1/DCTN1-p150[+]

(Ghosh-Roy et al., 2005)

NOT Enhanced by

Statement

Reference

ctp^exc39 has cystic bulge phenotype, non-enhanceable by Dhc64C^4-19

(Ghosh-Roy et al., 2005)

ctp^exc39 has nucleus & spermatid phenotype, non-enhanceable by Dhc64C^4-19

(Ghosh-Roy et al., 2005)

Suppressed by

Statement

Reference

ctp^exc39 has cystic bulge phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Hsap\DYNLL1^UAS.Tag:MYC

(Ghosh-Roy et al., 2005)

ctp^exc39 has actin filament bundle & nucleus phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Hsap\DYNLL1^UAS.Tag:MYC

(Ghosh-Roy et al., 2005)

Enhancer of

Statement

Reference

ctp^exc39 is an enhancer of cystic bulge phenotype of sw¹

(Ghosh-Roy et al., 2005)

ctp^exc39 is an enhancer of nucleus & spermatid phenotype of sw¹

(Ghosh-Roy et al., 2005)

Other

Statement

Reference

DCTN1-p150^Gl-1, ctp^exc39 has spermatocyte fusome phenotype

(Ghosh-Roy et al., 2004)

Df(3L)fz-GF3b/+, ctp^exc39 has spermatocyte fusome phenotype

(Ghosh-Roy et al., 2004)

Df(3L)GN24/+, ctp^exc39 has spermatocyte fusome phenotype

(Ghosh-Roy et al., 2004)

DCTN1-p150^Gl-1/DCTN1-p150[+], ctp^exc39 has spermatocyte fusome phenotype

(Ghosh-Roy et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

ctp^exc39; sw¹ mutants are viable at 25^oC.

The ctp^exc39/Y nuclear bundles and individualization complex phenotype is dominantly enhanced by Gl¹. The phenotype is also dominantly enhanced by Df(3L)GN24, which uncovers the Dhc64C gene. In contrast, the Dhc64C^4-19 allele does not affect this phenotype.

(Ghosh-Roy et al., 2005)

The sterility of ctp^exc39 males is further reduced when flies carry one copy of Gl¹ or Df(3L)fz-GF3b. The total number of elongation cones (ECs) per testis is reduced in ctp^exc39 mutants that carry one copy of Gl¹; some of these ECs are deformed and the average cyst length is shorter than in ctp^exc39 single mutants. The same phenotype occurs when ctp^exc39 flies carry one copy of Df(3L)GN24. In ctp^exc39; Df(3L)fz-GF3b/+ mutants, there are almost no detectable ECs.

(Ghosh-Roy et al., 2004)

Xenogenetic Interactions

Statement

Reference

Expression of Hsap\DNCL1^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} rescues the disruption of nuclear bundles and individualization complexes in ctp^exc39 mutant testis.

(Ghosh-Roy et al., 2005)

Complementation and Rescue Data

Rescued by

ctp^exc39 is rescued by Scer\GAL4^{VP16.nanos.UTR}/ctp^UASp.cGa

(Ghosh-Roy et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Allelic series in order of increasing strength based on testis phenotype: ctp^exc39 < ctp^ins1 < ctp^DIIA82.

(Ghosh-Roy et al., 2005)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ctp^exc39

ddlc1^exc39

(Ghosh-Roy et al., 2004)

exc39

(Ghosh-Roy et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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