FlyBase Allele Report: Dmel\Mcm10[d08029]

General Information

Symbol

Dmel\Mcm10^d08029

Species

D. melanogaster

Name

FlyBase ID

FBal0182781

Feature type

allele

Associated gene

Dmel\Mcm10

Associated Insertion(s)

P{XP}Mcm10^d08029

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - molecular evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - molecular evidence

(Reubens et al., 2015)

Mutagen

P-element activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

P{XP}Mcm10^d08029

Cytology

Description

Insertion of P{XP} into Mcm10, resulting in the removal of the C-terminal 71aa from Mcm10. The length of the truncated protein is 708aa as compared to the 776aa present in the full length Mcm10. Of the 708aa in the translated product, only 705aa are actually encoded by Mcm10 (the last 3aa are from P{XP}); the amino acids at the junction of Mcm10 and P{XP} are Gln Cys Met Met Lys STOP (Gln and Cys from Mcm10).

(Christensen, 2013.4.1)

Insertion in the second exon, which is predicted to truncate the C-terminus of the Mcm10protein by 85 amino acid residues. The insertion also adds the peptide DAEKRFS prior to encountering a stop codon within the intron.

(Apger et al., 2010)

Allele components

Component

Use(s)

Inserted element

P{XP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:21,182,197..21,182,197 [+]

(Apger et al., 2010, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle | larval stage (with Mcm10^Scim19)

(Apger et al., 2010)

decreased body size | recessive

(Apger et al., 2010)

increased occurrence of cell division | third instar larval stage

(Apger et al., 2010)

partially lethal - majority die | recessive

(Apger et al., 2010)

Phenotype Manifest In

polytene chromosome

(Apger et al., 2010)

polytene chromosome (with Mcm10^Scim19)

(Apger et al., 2010)

Detailed Description

Statement

Reference

Homozygous larvae are on average smaller than wild-type controls. Mutant pupae are highly variable in size (some are as large as wild type while others are only half the size of wild type_, but overall are 16% smaller than wild type.

Under-replication of polytene chromosomes is seen in mutant larvae, with underreplication being seen more often in mutant larvae which are small compared to larger larvae of the same genotype. Under-replication is seen in heterozygous, Mcm10^d08029/Mcm10^Scim19 and hemizygous larvae.

Mutant third instar larvae have slightly smaller brains than controls, and show a higher number of cells with of EdU incorporation.

Homozygous, heterozygous, hemizygous and Mcm10^d08029/Mcm10^Scim19 larvae have a smaller fraction of cells in mitosis in the brain than controls.

Nuclear divisions during cycles 10-12 occur with normal synchrony in homozygous embryos laid by homozygous females.

Mcm10^d08029 does not dominantly suppress position effect variegation of variegating dp allele.

(Apger et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mcm10^d08029 is rescued by Mcm10^3.5.Tag:FLAG

(Apger et al., 2010)

Comments

Mcm10^{3.5.T:Zzzz\FLAG} rescues the semi-lethality of Mcm10^d08029 homozygotes.

(Apger et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

85103

w¹¹¹⁸; P{XP}Mcm10^d08029

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Mcm10^d08029

(Reubens et al., 2015, Apger et al., 2010, Christensen et al., 2007)

d08029

(Christensen, 2013.4.1)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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