FlyBase Allele Report: Dmel\Mcm10[Scim19]

General Information

Symbol

Dmel\Mcm10^Scim19

Species

D. melanogaster

Name

FlyBase ID

FBal0246784

Feature type

allele

Associated gene

Dmel\Mcm10

Associated Insertion(s)

P{SUPor-P}Mcm10^Scim19

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

hypomorphic allele - molecular evidence

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Apger et al., 2010, Christensen et al., 2007)

hypomorphic allele - molecular evidence

(Apger et al., 2010)

Mutagen

Delta2-3 transposase

(Dobie et al., 2001)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Mcm10^Scim19

Cytology

Description

A P{SUPor-P} insertion 76bp 5 prime of the start codon for annotation CG9241 of Mcm10.

(Christensen and Karpen, 2002.4.22)

Insertion of P{SUPor-P} into Mcm10.

(Dobie et al., 2001)

Allele components

Component

Use(s)

Inserted element

P{SUPor-P}

insertional mutagenesis tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

condensed nuclear chromosome

(Christensen et al., 2007)

larval brain

(Apger et al., 2010)

nurse cell

(Apger et al., 2010)

polytene chromosome (with Mcm10^d08029)

(Apger et al., 2010)

Detailed Description

Statement

Reference

Polytene chromosomes of mutant larvae appear normal.

Mutant third instar larvae have slightly smaller brains than controls, and show a higher number of cells with of EdU incorporation.

Under-replication of polytene chromosomes is seen in Mcm10^d08029/Mcm10^Scim19 larvae.

Homozygous, heterozygous, hemizygous and Mcm10^d08029/Mcm10^Scim19 larvae have a smaller fraction of cells in mitosis in the brain than controls.

Nuclear divisions during cycles 10-12 are asynchronous in homozygous embryos laid by homozygous females. Anaphase bridges are seen in 29% of the embryos in cell cycles 10-12.

Mutant egg chambers show temporal defects in decondensation of the nurse cell chromosomes; nurse cell nuclei in stage 7 mutant egg chambers are heterogeneous with respect to chromosome decondensation with some nuclei fully decondensed and other persisting in the five-blob stage (this persistence is seen in nearly 75% of egg chambers).

Mcm10^Scim19 dominantly suppresses position effect variegation of variegating dp allele.

(Apger et al., 2010)

Mcm10^Scim19 mutants show defective chromosome condensation.

(Christensen et al., 2007)

Dominantly decreases transmission of Dp(1;f)J21A to offspring to 17%.

(Dobie et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Mcm10^Scim19 is rescued by Mcm10^3.5.Tag:FLAG

(Apger et al., 2010)

Comments

Mcm10^{3.5.T:Zzzz\FLAG} rescues the semi-lethality of Mcm10^Scim19 homozygotes.

(Apger et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Mcm10¹

Mcm10^Scim19

(Apger et al., 2010, Christensen et al., 2007)

Scim19¹

Name Synonyms

Secondary FlyBase IDs

FBal0193426
FBal0123078

References (4)

Report Sections

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