FlyBase Allele Report: Dmel\br[Z3.UAS]

General Information

Symbol

Dmel\br^Z3.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0189741

Feature type

allele

Associated gene

Dmel\br

Associated Insertion(s)

Carried in Construct

P{UAS-br.Z3}

Also Known As

UAS-br-Z3

Key Links

Transgenic product class

cDNA

(Zhou et al., 2009)

wild_type

(Zhou et al., 2009)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Zhou et al., 2009)

wild_type

(Zhou et al., 2009)

Mutagen

in vitro construct

(Zhou et al., 2009, Schubiger et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-br.Z3}

Cytology

Description

UASt regulatory sequences drive expression of cDNA clone "dm797" which encodes the br Z3 isoform.

(Zhou et al., 2009)

UAS regulatory sequences drive expression of br-Z3 isoform coding sequences.

(Schubiger et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

UASt

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | first instar larval stage, with Scer\GAL4^elav-C155

(Zhou et al., 2009)

abnormal locomotor behavior | second instar larval stage, with Scer\GAL4^elav-C155

(Zhou et al., 2009)

abnormal neuroanatomy | adult stage, with Scer\GAL4^P2.4.Pdf

(Zhou et al., 2009)

lethal, with Scer\GAL4^Feb211

(Zhou et al., 2004)

lethal, with Scer\GAL4^P0206

(Zhou et al., 2004)

lethal | embryonic stage, with Scer\GAL4^elav-C155

(Zhou et al., 2009)

lethal | first instar larval stage, with Scer\GAL4^elav-C155

(Zhou et al., 2009)

lethal | pharate adult stage, with Scer\GAL4^Feb204

(Zhou et al., 2004)

lethal | pupal stage P7, with Scer\GAL4^Feb204

(Zhou et al., 2004)

Phenotype Manifest In

LNv neuron, with Scer\GAL4^P2.4.Pdf

(Zhou et al., 2009)

prothoracic gland, with Scer\GAL4^P0206

(Zhou et al., 2004)

Detailed Description

Statement

Reference

Animals expressing br^Z3.Scer\UAS under the control of Scer\GAL4^elav-C155 that hatch into larvae show normal morphology but sluggish movements. Most fail to feed and die during the first instar. Some larvae are able to feed and survive to the second instar, but they show little coordinated movement.

Less than 1% of larvae expressing br^Z3.Scer\UAS (using the P{UAS-br.Z3}216-1 line) under the control of Scer\GAL4^elav-C155 hatch, and they show reduced crawling even after stimulation.

Unhatched embryos expressing br^Z3.Scer\UAS under the control of Scer\GAL4^elav-C155 have no gross anatomical defects.

Animals expressing br^Z3.Scer\UAS under the control of Scer\GAL4^P2.4.Pdf show abnormalities in the LN[[v]] neurons at metamorphosis, with the neurons in the mutant animals having a diffuse spray of neurites that extend to the midline (in contrast to the compact projection pattern of wild-type neurons which terminates well before the midline).

(Zhou et al., 2009)

Animals expressing br^Z3.Scer\UAS under the control of Scer\GAL4^Feb211 undergo normal larval and pupal moults. However, these animals fail to eclose properly, with their abdomens remaining in the puparial cases.

Most animals expressing br^Z3.Scer\UAS under the control of Scer\GAL4^Feb204 form puparia. All of the prepupae successfully pupate, with approximately 18% of them arrested at stage P7, 12% arrested as pharate adults, 14% partially eclosed and 56% fully eclosed.

Approximately 53% of larvae expressing br^Z3.Scer\UAS under the control of Scer\GAL4^P0206 become permanent first instar larvae which survive for 4-6 days. Of those larvae that ecdyse to the second instar, 81% are permanently arrested in the second instar, 19% ecdyse to the third instar and 1% form "L2 prepupae" which are characterised by their uneverted, single club-shaped anterior spiracles and small mouthhooks with a few teeth (characteristic of wild-type second instar larvae).

Expression of br^Z3.Scer\UAS under the control of Scer\GAL4^P0206 causes degeneration of the prothoracic gland portion of the ring gland by 2 days after ecdysis to the second instar. The corpora allata are unaffected.

63% of animals expressing br^Z3.Scer\UAS under the control of Scer\GAL4^12.1 remain as first or second instar larvae.

(Zhou et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

br^Z3.UAS/Scer\GAL4^GMR11F02 is a non-suppressor of abnormal thermotaxis | larval stage phenotype of Dgat2^KO

(Deng et al., 2025)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51192

w¹¹¹⁸; P{UAS-br.Z3}216-1

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

br^Z3.Scer\UAS

br^Z3.UAS

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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