FlyBase Allele Report: Dmel\hpo[Δauto.UAS]

General Information

Symbol

Dmel\hpo^Δauto.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0191336

Feature type

allele

Associated gene

Dmel\hpo

Associated Insertion(s)

Carried in Construct

P{UAS-hpo.Δauto}

Key Links

Transgenic product class

gain_of_function_variant

(Udan et al., 2003)

inframe_deletion

(Udan et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

gain_of_function_variant

(Udan et al., 2003)

inframe_deletion

(Udan et al., 2003)

Mutagen

in vitro construct

(Udan et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-hpo.Δauto}

Cytology

Description

UASt regulatory sequences drive expression of a hyperactive form of hpo that carries a deletion which removes the autoregulatory domain (amino acid residues 355-465 are missing). This deletion is analogous to a deletion in human MST1 that causes an increase in kinase activity in vitro.

(Udan et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

hpo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^nub-AC-62

(Verghese et al., 2012)

Phenotype Manifest In

wing disc, with Scer\GAL4^nub-AC-62

(Verghese et al., 2012)

Detailed Description

Statement

Reference

Overexpression of hpo^Scer\UAS.cUa under the control of Scer\GAL4^nub-AC-62 causes extensive cell death in the wing discs, and results in the development of a small-wing phenotype.

(Verghese et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^nub-AC-62, hpo^Δauto.UAS has increased cell death phenotype, suppressible by Dronc^NIG.8091R, Scer\GAL4^nub-AC-62

(Verghese et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^nub-AC-62, hpo^Δauto.UAS has wing disc phenotype, suppressible by Dronc^NIG.8091R, Scer\GAL4^nub-AC-62

(Verghese et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Nc^NIG.8091R with hpo^{Δauto.Scer\UAS} in the developing wing discs (under the control of Scer\GAL4^nub-AC-62) dramatically reduces the level of cell death, with a concomitant rescue of adult wing size.

(Verghese et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

UAS-hpo^Δauto

(Udan et al., 2003)

hpo^{Δauto.Scer\UAS}

hpo^Δauto.UAS

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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