FlyBase Allele Report: Dmel\Irc[RNAi.UAS]

General Information

Symbol

Dmel\Irc^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0191969

Feature type

allele

Associated gene

Dmel\Irc

Associated Insertion(s)

Carried in Construct

P{UAS-Irc.RNAi}

Also Known As

UAS-IRC-RNAi

Key Links

Transgenic product class

RNAi_reagent

(Ha et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ha et al., 2005)

Mutagen

in vitro construct

(Ha et al., 2005, Ha et al., 2005, Ha et al., 2005, Ha et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-Irc.RNAi}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat of a 510bp cDNA fragment encoding amino acids 68-238 of Irc. The repeated copies of this 510bp fragment are separated by an intronic spacer.

(Ha et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Irc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response, with Scer\GAL4^cad-em459

(Ha et al., 2005)

abnormal immune response, with Scer\GAL4^cad-md509

(Ha et al., 2005)

abnormal immune response, with Scer\GAL4^da.PU

(Ha et al., 2005)

chemical sensitive, with Scer\GAL4^da.PU

(Ha et al., 2005)

Phenotype Manifest In

Detailed Description

Statement

Reference

Flies expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show reduced survival compared to control flies after natural infection with either live or heat killed "Ecc-15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

In the presence of hydrogen peroxide, the survival rates of flies expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 are significantly lower than those of control flies. Animals expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 show reduced survival after natural infection (feeding with contaminated food) compared to control flies. This effect has been seen with each of the following microorganisms; "Ecc-15" (P.carotovorum.carotovorum), "Ecc-15-evf^-/^-, E.coli, S.cerevisiae, M.luteus and S.typhimurium. Animals expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-md509 show reduced survival after natural infection with "Ecc-15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

Flies expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 show a reduced survival rate compared to control flies when naturally infected with "Ecc15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Irc^RNAi.UAS, Scer\GAL4^da.PU has abnormal immune response phenotype, suppressible | partially by puc^E69/puc[+]

(Ha et al., 2005)

Irc^RNAi.UAS, Scer\GAL4^da.PU has abnormal immune response phenotype, suppressible | partially by Cat^{Tag:Mito(Oat)}

(Ha et al., 2005)

Irc^RNAi.UAS, Scer\GAL4^da.PU has abnormal immune response phenotype, suppressible | partially by Cat^UAS.cAa, Scer\GAL4^da.PU

(Ha et al., 2005)

Irc^RNAi.UAS, Scer\GAL4^da.PU has abnormal immune response phenotype, suppressible by Hsap\CAT^UAS.cHa, Scer\GAL4^da.PU

(Ha et al., 2005)

Irc^RNAi.UAS, Scer\GAL4^cad-em459 has abnormal immune response phenotype, suppressible by Duox^RNAi.UAS, Scer\GAL4^cad-em459

(Ha et al., 2005)

Suppressor of

Statement

Reference

Irc^RNAi.UAS/Scer\GAL4^da.G32 is a suppressor | partially of short lived | conditional phenotype of Hayan¹

(Nam et al., 2012)

Irc^RNAi.UAS/Scer\GAL4^ChAT.7.4 is a suppressor | partially of short lived | conditional phenotype of Hayan¹

(Nam et al., 2012)

Irc^RNAi.UAS, Scer\GAL4^cad-em459 is a suppressor of abnormal immune response phenotype of Duox^RNAi.UAS, Scer\GAL4^cad-em459

(Ha et al., 2005)

NOT Suppressor of

Statement

Reference

Irc^RNAi.UAS/Scer\GAL4^He.PZ is a non-suppressor of short lived | conditional phenotype of Hayan¹

(Nam et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 partially suppresses the wound-induced mortality seen in Hayan¹ mutants. The mortality is also rescued when Irc^{dsRNA.Scer\UAS} is expressed in neurons under the control of Scer\GAL4^Cha.7.4, but not when it is expressed in hemocytes under the control of Scer\GAL4^He.PZ.

(Nam et al., 2012)

The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 is partially rescued by puc^E69/+. The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 is partially rescued by co-expression of either Cat^T:mito1 or Cat^Scer\UAS.cMa. The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing Irc^{dsRNA.Scer\UAS} under the control of Scer\GAL4^da.G32 is slightly rescued by co-expression of Jafrac1^Scer\UAS.cHa.

(Ha et al., 2005)

Flies co-expressing Irc^{dsRNA.Scer\UAS} and Duox^{dsRNA.Scer\UAS} under the control of Scer\GAL4^cad-em459 show a normal survival rate when naturally infected with "Ecc15" (P.carotovorum.carotovorum).

(Ha et al., 2005)

Xenogenetic Interactions

Statement

Reference

(Ha et al., 2005)

Complementation and Rescue Data

Comments

(Ha et al., 2005)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Irc^RNAi.UAS

Irc^{dsRNA.Scer\UAS}

Irc^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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