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General Information
Symbol
Dmel\IrcdsRNA.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0191969
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-IRC-RNAi
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of an inverted repeat of a 510bp cDNA fragment encoding amino acids 68-238 of Irc. The repeated copies of this 510bp fragment are separated by an intronic spacer.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 show reduced survival compared to control flies after natural infection with either live or heat killed "Ecc-15" (P.carotovorum.carotovorum).

In the presence of hydrogen peroxide, the survival rates of flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 are significantly lower than those of control flies. Animals expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 show reduced survival after natural infection (feeding with contaminated food) compared to control flies. This effect has been seen with each of the following microorganisms; "Ecc-15" (P.carotovorum.carotovorum), "Ecc-15-evf-/-, E.coli, S.cerevisiae, M.luteus and S.typhimurium. Animals expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4cad-md509 show reduced survival after natural infection with "Ecc-15" (P.carotovorum.carotovorum).

Flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4cad-em459 show a reduced survival rate compared to control flies when naturally infected with "Ecc15" (P.carotovorum.carotovorum).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Suppressed by
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

IrcdsRNA.UAS/Scer\GAL4He.PZ is a non-suppressor of short lived | conditional phenotype of Hayan1

Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

Expression of IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 partially suppresses the wound-induced mortality seen in Hayan1 mutants. The mortality is also rescued when IrcdsRNA.Scer\UAS is expressed in neurons under the control of Scer\GAL4Cha.7.4, but not when it is expressed in hemocytes under the control of Scer\GAL4He.PZ.

The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 is partially rescued by pucE69/+. The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 is partially rescued by co-expression of either CatT:mito1 or CatScer\UAS.cMa. The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 is slightly rescued by co-expression of Jafrac1Scer\UAS.cHa.

Flies co-expressing IrcdsRNA.Scer\UAS and DuoxdsRNA.Scer\UAS under the control of Scer\GAL4cad-em459 show a normal survival rate when naturally infected with "Ecc15" (P.carotovorum.carotovorum).

Xenogenetic Interactions
Statement
Reference

The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 is rescued by co-expression of Hsap\CATScer\UAS.cHa.

Complementation and Rescue Data
Comments

The reduced survival rate after natural infection with "Ecc-15" (P.carotovorum.carotovorum) that is seen in flies expressing IrcdsRNA.Scer\UAS under the control of Scer\GAL4da.G32 is rescued by co-expression of IrcScer\UAS.cHa.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
IrcdsRNA.Scer\UAS
IrcdsRNA.UAS
Name Synonyms
Secondary FlyBase IDs
    References (5)