FB2026_02 , released June 18, 2026
Allele: Dmel\Gr66aUAS.Tag:MYC
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General Information
Symbol
Dmel\Gr66aUAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0194163
Feature type
allele
Associated gene
Dmel\Gr66a
Associated Insertion(s)
Carried in Construct
P{UAS-Gr66a.MYC}
Also Known As
UAS-Gr66a
Key Links
Transgenic product class
wild_type
(Moon et al., 2006)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Moon et al., 2006)
Mutagen
in vitro construct
(Moon et al., 2006)
Progenitor genotype
Carried in construct
P{UAS-Gr66a.MYC}
Cytology
Description

UAS regulatory sequences drive expression of the full Gr66a coding region, tagged with Tag:MYC.

(Moon et al., 2006)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Gr66a
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The expression of Gr66aUAS.Tag:MYC under the control of Scer\GAL4Gr64f.9.7 is not sufficient to make L-type sensilla responsive to the bitter chemicals LOB, BER and DEN.

      (Sung et al., 2017)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      NOT suppressed by
      Other
      Statement
      Reference

      Gr32aUAS.cSa, Gr33aUAS.cMa, Gr39aUAS.a.cSa, Gr59cUAS.cUa, Gr66aUAS.Tag:MYC, Gr89aUAS.cSa, Scer\GAL4Gr64f.9.7 has abnormal neurophysiology | adult stage phenotype

      (Sung et al., 2017)

      Gr32aUAS.cSa, Gr33aUAS.cMa, Gr39aUAS.a.cSa, Gr59cUAS.cUa, Gr66aUAS.Tag:MYC, Gr89aUAS.cSa, Scer\GAL4Gr64f.9.7 has abnormal taste perception | adult stage phenotype

      (Sung et al., 2017)

      Gr22eUAS.cSa, Gr32aUAS.cSa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal taste perception | adult stage phenotype

      (Sung et al., 2017)

      Df(3L)ΔGr64af, Gr22eUAS.cSa, Gr32aUAS.cSa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal neurophysiology | adult stage phenotype

      (Sung et al., 2017)

      Df(3L)ΔGr64af, Gr22eUAS.cSa, Gr32aUAS.cSa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal taste perception | adult stage phenotype

      (Sung et al., 2017)

      Gr32aUAS.cSa, Gr59cUAS.cUa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal neurophysiology | adult stage phenotype

      (Sung et al., 2017)

      Gr32aUAS.cSa, Gr59cUAS.cUa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal taste perception | adult stage phenotype

      (Sung et al., 2017)

      Df(3L)ΔGr64af, Gr32aUAS.cSa, Gr59cUAS.cUa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal neurophysiology | adult stage phenotype

      (Sung et al., 2017)

      Df(3L)ΔGr64af, Gr32aUAS.cSa, Gr59cUAS.cUa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal taste perception | adult stage phenotype

      (Sung et al., 2017)

      Gr22eUAS.cSa, Gr32aUAS.cSa, Gr66aUAS.Tag:MYC, Scer\GAL4Gr64f.9.7 has abnormal neurophysiology | adult stage phenotype

      (Sung et al., 2017)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The sextuple co-expression of Gr32aUAS.cSa, Gr33aUAS.cMa, Gr39aUAS.a.cSa, Gr59cUAS.cUa, Gr66aUAS.Tag:MYC and Gr89aUAS.cSa under the control of Scer\GAL4Gr64f.9.7 makes L-type sensilla responsive to the bitter chemicals LOB, BER, and DEN, but not to the bitter chemicals CAF, STR, SOA or UMB. Control L-type sensilla, however, are only responsive to sucrose; this sextuple co-expression has no effect on the normal response to sucrose.

      Only quintuple co-expression combinations that include Gr32aUAS.cSa, Gr59cUAS.cUa and Gr66aUAS.Tag:MYC induce responsiveness of L-type sensilla to LOB, BER, and DEN. In agreement, the triple co-expression of Gr32aUAS.cSa, Gr59cUAS.cUa and Gr66aUAS.Tag:MYC is sufficient to induce responsiveness to LOB, BER, and DEN, but not to CAF, STR, SOA, or UMP, whereas all double combinations of these three do not. All mentioned quintuple, triple and double co-expression have no effect on the normal response to sucrose.

      L-type sensilla co-expressing Gr32aUAS.cSa, Gr59cUAS.cUa and Gr66aUAS.Tag:MYC under the control of Scer\GAL4Gr64f.9.7 show the expected responsiveness to sucrose and ectopic responsiveness to the bitter chemicals LOB, BER and DEN. Df(3L)ΔGr64af abolishes the expected responsiveness to sucrose, but not the bitter chemicals.

      The triple co-expression of Gr32aUAS.cSa, Gr66aUAS.Tag:MYC and Gr22eUAS.cSa under the control of Scer\GAL4Gr64f.9.7 makes L-type sensilla responsive to the bitter chemicals LOB, BER, DEN, STR and UMB, but not to CAF or SOA, while not affecting the normal response to sucrose. Df(3L)ΔGr64af abolishes the responsiveness to sucrose, reduces the ectopic responsiveness to STR, but has no effect on the ectopic responsiveness to LOB, BER and DEN.

      The triple co-expression of Gr32aUAS.cSa, Gr66aUAS.Tag:MYC and either Gr22fUAS.cSa or Gr39bUAS.cSa under the control of Scer\GAL4Gr64f.9.7 does not confer responsiveness of L-type sensilla to the bitter chemicals LOB, BER or DEN.

      (Sung et al., 2017)

      As in control flies, co-expression of Gr32aScer\UAS.cMa, Gr33aScer\UAS.cMa, Gr47aScer\UAS.cLa and Gr66aScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Gr5a.PUn does not elicit action potentials in sugar-responsive gustatory receptor neurons upon application of strychnine.

      (Lee et al., 2015)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Gr66aUAS.Tag:MYC/Scer\GAL4Gr66a.PD rescues Df(3L)ex83

      (Moon et al., 2006)
      Partially rescues

      Gr66aUAS.Tag:MYC/Scer\GAL4Gr66a.PD partially rescues Df(3L)ex83

      (Poudel et al., 2015)
      Comments

      Expression of Gr66aScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Gr66a.PD in the Df(3L)ex83 homozygote adults rescues the perturbed avoidance of umbelliferone in a two-way choice feeding assay as well as the site selection for oviposition assay and partially restores the reduction in frequency of action potentials invoked in the S6 sensilla upon umbelliferone exposure.

      (Poudel et al., 2015)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      Gr66aScer\UAS.T:Hsap\MYC
      Gr66aUAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (7)