FlyBase Allele Report: Dmel\aux[K47]

General Information

Symbol

Dmel\aux^K47

Species

D. melanogaster

Name

FlyBase ID

FBal0197315

Feature type

allele

Associated gene

Dmel\aux

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Eun et al., 2007)

Progenitor genotype

Cytology

Description

Amino acid replacement: C671Y.

(Eun et al., 2007)

Nucleotide substitution: G?A.

(Eun et al., 2007)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:4,225,188..4,225,188 [+]

Nucleotide change:

G4225188A

Reported nucleotide change:

G?A

Amino acid change:

C671Y | aux-PA; C671Y | aux-PB; C671Y | aux-PC; C663Y | aux-PD

Reported amino acid change:

C671Y

Comment:

Reported as a TGT to TAT nonsense mutation.

(Eun et al., 2007)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal (with aux^D128)

(Banks et al., 2011)

lethal | cold sensitive (with aux^L7)

(Eun et al., 2007)

viable (with aux^D128)

(Eun et al., 2008)

visible (with aux^D128)

(Eun et al., 2008)

Phenotype Manifest In

eye (with aux^D128)

(Banks et al., 2011)

eye photoreceptor cell (with aux^D128)

(Eun et al., 2007)

eye photoreceptor cell | ectopic (with aux^D128)

(Eun et al., 2007)

ommatidium (with aux^D128)

(Banks et al., 2011, Eun et al., 2008, Eun et al., 2007)

wing (with aux^D128)

(Eun et al., 2007)

wing blade (with aux^D128)

(Eun et al., 2008)

wing vein | ectopic (with aux^D128)

(Eun et al., 2007)

Detailed Description

Statement

Reference

aux^D128/aux^K47 adult escapers exhibit severely malformed imaginal disc-derived structures, including their eyes.

(Banks et al., 2011)

Only approximately 25% of facets in aux^D128/aux^K47 flies are wild type.

aux^D128/aux^K47 flies show notching of the wing.

(Eun et al., 2008)

aux^L7/aux^K47 animals are viable at 25^oC but do not survive at 18^oC. The escapers have wild-type wings.

aux^K47/aux^D128 escapers have rough eyes with disorganised ommatidia. Many of the ommatidia have too many photoreceptor cells, some have too few and some ommatidia are fused.

aux^K47/aux^D128 escapers have extra vein material in the wings and the wings are notched.

(Eun et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

aux^K47/aux^D128 has lethal phenotype, suppressible | partially by lqf^GFP

(Banks et al., 2011)

aux^K47/aux^D128 has lethal phenotype, suppressible | partially by lqf^GFP/Chc^+t14.3

(Banks et al., 2011)

aux^K47/aux^D128 has lethal phenotype, suppressible | partially by Chc^+t14.3

(Banks et al., 2011)

aux^K47/aux^D128 has visible phenotype, suppressible by Chc^+t14.3

(Eun et al., 2008)

Enhancer of

Statement

Reference

aux^K47/aux[+] is an enhancer of visible phenotype of lqf^{glrs.Tag:FLAG}

(Eun et al., 2007)

Other

Statement

Reference

Chc¹, aux^K47/aux^D128 has lethal | dominant phenotype

(Eun et al., 2008)

aux^K47/aux^L7, lqf^ARI has visible phenotype

(Eun et al., 2007)

Phenotype Manifest In

Suppressed by

Statement

Reference

aux^K47/aux^D128 has eye phenotype, suppressible | partially by Chc^+t14.3

(Banks et al., 2011)

aux^K47/aux^D128 has eye phenotype, suppressible | partially by lqf^GFP

(Banks et al., 2011)

aux^K47/aux^D128 has ommatidium phenotype, suppressible | partially by lqf^GFP

(Banks et al., 2011)

aux^K47/aux^D128 has eye phenotype, suppressible by lqf^GFP/Chc^+t14.3

(Banks et al., 2011)

aux^K47/aux^D128 has ommatidium phenotype, suppressible by lqf^GFP/Chc^+t14.3

(Banks et al., 2011)

aux^K47/aux^D128 has ommatidium phenotype, suppressible | partially by Chc^+t14.3

(Banks et al., 2011, Eun et al., 2008)

aux^K47/aux^D128 has wing blade phenotype, suppressible by Chc^+t14.3

(Eun et al., 2008)

Enhancer of

Statement

Reference

aux^K47/aux[+] is an enhancer of eye phenotype of lqf^{glrs.Tag:FLAG}

(Eun et al., 2007)

Other

Statement

Reference

aux^K47/aux^L7, lqf^ARI has wing phenotype

(Eun et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

Addition of Chc^+t14.3 to a aux^D128/aux^K47 background increases the eclosion frequency of adults markedly. The mutant eye phenotype of those rescued animals is suppressed somewhat (from 8% to 28% wild-type ommatidia).

Addition of lqf^{+t16240.T:Avic\GFP} to a aux^D128/aux^K47 background increases the eclosion frequency of adults markedly. The mutant eye phenotype of escaper aux^D128/aux^K47 flies is also suppressed (from 8% to 62% wild-type ommatidia).

aux^D128/aux^K47 mutant flies carrying both Chc^+t14.3 and lqf^{+t16240.T:Avic\GFP} exhibit remarkably normal-appearing eyes (97% wild-type ommatidia). However, no increase in viability is seen in these flies above the level observed with lqf^{+t16240.T:Avic\GFP} alone.

(Banks et al., 2011)

Chc¹/+ ; aux^D128/aux^K47 adults cannot be recovered.

Chc^+t14.3 completely suppresses the mutant wing phenotype of aux^D128/aux^K47 flies and significantly suppresses the mutant facet phenotype of aux^D128/aux^K47 flies.

(Eun et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

aux^K47/aux^D128 is rescued by Scer\GAL4^Act5C.PI/aux^UAS.cHa

(Eun et al., 2008)

aux^K47/aux^D128 is rescued by aux^+t21

(Eun et al., 2008)

aux^K47/aux^D128 is rescued by aux^ro.PE

(Eun et al., 2008)