Open Close
General Information
Symbol
Dmel\Sec151
Species
D. melanogaster
Name
FlyBase ID
FBal0197840
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

A21107421T

Amino acid change:

K137term | Sec15-PA

Reported amino acid change:

K137term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Revision of amino acid replacement data reported in FBrf0188181.

Amino acid replacement: K137term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
ameliorates  cancer
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

axon & photoreceptor cell R7 | somatic clone

axon & photoreceptor cell R8 | somatic clone

nerve terminal & optic cartridge | somatic clone

Detailed Description
Statement
Reference

Sec151 mutant clones do not affect cell proliferation in eye-antenna imaginal discs and the size of Sec151 mutant clones is comparable to wild type. Sec151 mutant cells persist into the adult eye.

Neuronal differentiation is normal in sec151 homozygous clones in the developing eye.

Flies whose eyes are homozygous for sec151 (due to somatic clones induced using Scer\FRT and Scer\FLP1ey.PN) exhibit reduce phototaxis in response to red and blue light. Electro-retinograms of these animals show normal depolarization in response to light but an absence of on and off transients. Projections from photoreceptors are aberrant in the eyes of these flies: there is not regular array of terminals in the medulla and R7 and R8 target layering is highly aberrant. Neuropils in the optic lobes of these flies are abnormally shaped, but their size and arrangement are normal. On a gross level, long range pathfinding, extension and targeting of R7 axons appears to be normal. However R7 terminals fail to establish normal, local wiring patterns within their target neuropils.

Most wild-type lamina cartridges have 6 photoreceptor terminals per cartridge, with a few showing 5, very occasionally, 7. Flies whose eyes are homozygous for sec151 (due to somatic clones induced using Scer\FRT and Scer\FLP1ey.PN), numbers of photoreceptor terminals per cartridge range from 2-8 and are relatively evenly distributed throughout this range (4-5 is most common, 2 less). However, these terminals form morphologically normal synapses in normal numbers per terminal. When somatic clones of sec151 homozygous cells are instead induced using Scer\FLP1ey.3.5.hs, mutant optic lobes still exhibit neuronal targeting defects but the distribution of terminal numbers per cartridge is less broad and on/off transients in electro-retinograms are reduced rather than eliminated.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressor of
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

Sec151/+;Arfip71/+ third instar larvae do not show a reduction in bouton number at the neuromuscular junction, compared to Sec151/+ heterozygotes.

Sec151 dramatically suppresses the overgrowth phenotype and pupal lethality seen in eye-antennal disc clones expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU. The increased secretion (dye clearance) seen in cells expressing Ras85DV12.Scer\UAS is also suppressed by Sec151. Cell death is no longer detected in the wild type cells surrounding the clones.

Expression of bskDN.Scer\UAS suppresses the increased cell death seen in eye-antennal disc clones expressing Ras85DV12.Scer\UAS under the control of Scer\GAL4Act.PU in a Sec151 mutant background. Tumor growth is not restored to that seen in clones expressing Ras85DV12.Scer\UAS alone.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (10)