FlyBase Allele Report: Dmel\Gr28b[MB03888]

General Information

Symbol

Dmel\Gr28b^MB03888

Species

D. melanogaster

Name

FlyBase ID

FBal0198336

Feature type

allele

Associated gene

Dmel\Gr28b

Associated Insertion(s)

Mi{ET1}Gr28b^MB03888

Carried in Construct

Also Known As

Gr28b^Mi, Gr28b¹

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}Gr28b^MB03888

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:7,455,506..7,455,506 [-]

(Meiselman et al., 2022, Dhakal et al., 2021, Rimal et al., 2019, Sang et al., 2019, Zhang et al., 2014, Ni et al., 2013, Xiang et al., 2010, Bellen, 2007.8.20)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult antennal lobe projection neuron VP5+VP2 l2PN

(Liu et al., 2015)

adult antennal lobe projection neuron VP5+VP2 l2PN | drug conditional

(Liu et al., 2015)

adult multiglomerular antennal lobe projection neuron VP4+VP2+VL1 l2PN | drug conditional

(Liu et al., 2015)

Detailed Description

Statement

Reference

Gr28b^MB03888 mutant flies do not show significant changes in the expected avoidance response to food laced with acetic acid (5%), as compared to controls.

(Rimal et al., 2019)

Gr28b^MB03888 mutant larvae have slightly reduced rolling (escape) behavior in response to noxious temperature increases, compared to controls.

(Luo et al., 2017)

Gr28b^MB03888 mutants show the expected S-b sensilla responsiveness to the bitter chemicals LOB, BER, DEN.

(Sung et al., 2017)

Gr28b^MB03888 mutant adults do not have any changes in food intake under fed or starved conditions, compared to controls.

(Zhan et al., 2016)

Gr28b^MB03888 homozygous mutant adults do not have any substantial alterations in the responses of fast-cool or slow-cool projection neurons (PNs) to temperature changes, compared to controls; warm PNs have non-significantly reduced responses to slow, but not fast, warming, compared to controls and their responses to small warming steps are significantly reduced; unlike controls, the responses of warm PNs in Gr28b^MB03888/Gr28b^MB03888 mutants to fast warming or small warming steps are abolished when inhibition is blocked using picrotoxin and CGP54626.

In the absence of drugs, warm-cool PNs in Gr28b^MB03888/Gr28b^MB03888 mutant adults do not have any substantial alterations in responses to cooling or warming, including to small temperature steps, compared to controls; unlike in controls, warm-cool PNs in mutants lose their responses to warming, but not cooling, when inhibition is blocked using picrotoxin and CGP54626.

(Liu et al., 2015)

Gr28b^MB03888/Df(2L)Exel7031 mutant flies show complete loss of the "rapid negative thermotaxis" (response to a steep temperature gradient after 1 min) seen in wild type flies. When flies are exposed to a shallow gradient for 30 minutes the flies behave similarly to wild type.

(Ni et al., 2013)

Gr28b^MB03888/Df(2L)Exel7031 mutants exhibit a significant reduction in the light-induced response of class IV dendritic arborization neurons. However, the basal firing rate is not affected.

(Xiang et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gr28b^MB03888 has abnormal sensory perception phenotype, enhanceable by TrpA1[+]/TrpA1^unspecified

(Xiang et al., 2010)

Suppressed by

Statement

Reference

Gr28b^MB03888 has abnormal temperature response phenotype, suppressible | partially by Scer\GAL4^Gr28b.d.PT/TrpA1^UAS.(B).cKa

(Ni et al., 2013)

NOT suppressed by

Statement

Reference

Gr28b^MB03888 has abnormal temperature response phenotype, non-suppressible by TrpA1^UAS.(A).cKa/Scer\GAL4^Gr28b.d.PT

(Ni et al., 2013)

Enhancer of

Statement

Reference

Gr28b^MB03888/Gr28b[+] is an enhancer of abnormal sensory perception phenotype of TrpA1^unspecified

(Xiang et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of TrpA1^{Scer\UAS.(B).cKa} in the arista under the control of Scer\GAL4^Gr28b.d.PT significantly rescues the "rapid negative thermotaxis" defects seen in Gr28b^MB03888 mutant flies.

Expression of TrpA1^{Scer\UAS.(A).cKa} in the arista under the control of Scer\GAL4^Gr28b.d.PT does not rescue the "rapid negative thermotaxis" defects seen in Gr28b^MB03888 mutant flies.

(Ni et al., 2013)

Transheterozygotes carrying one mutant allele of TrpA1^unspecified and one copy of Gr28b^MB03888 exhibit a reduced light response, suggesting that Gr28b and TrpA1 function in the same phototransduction pathway.

(Xiang et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Gr28b^MB03888 is rescued by Scer\GAL4^Gr28b.d.PT/Gr28b^UAS.E

(Ni et al., 2013)

Gr28b^MB03888 is rescued by Gr28b^UAS.D/Scer\GAL4^Gr28b.d.PT

(Ni et al., 2013)

Gr28b^MB03888 is rescued by Gr28b^UAS.D/Scer\GAL4^hot-cell

(Ni et al., 2013)

Gr28b^MB03888 is rescued by Gr28b^+t14.5

(Ni et al., 2013)

Comments

Expression of Gr28b^Scer\UAS.D under the control of either Scer\GAL4^Gr28b.d.PT or Scer\GAL4^hot-cell rescues the "rapid negative thermotaxis" defects seen in Gr28b^MB03888 mutant flies.

Expression of Gr28b^+t14.5 rescues the "rapid negative thermotaxis" defects seen in Gr28b^MB03888 mutant flies.

(Ni et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

24190

w¹¹¹⁸; Mi{ET1}Gr28b^MB03888

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gr28b¹

(Rimal and Lee, 2019, Rimal et al., 2019, Poudel et al., 2017)

Gr28b^MB03888

(Lamaze et al., 2017, Luo et al., 2017, Liu et al., 2015, Zhang et al., 2014, Bellen, 2007.8.20)

Gr28b^Mi

(Dhakal et al., 2021, Sang et al., 2019, Ni et al., 2016, Zhan et al., 2016, Ni et al., 2013)

Gr28b^mi

(Sung et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (17)

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