FlyBase Allele Report: Dmel\SerRS-m[GD12798]

General Information

Symbol

Dmel\SerRS-m^GD12798

Species

D. melanogaster

Name

FlyBase ID

FBal0198630

Feature type

allele

Associated gene

Dmel\SerRS-m

Associated Insertion(s)

Carried in Construct

P{GD12798}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12798}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12798

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates cancer

modeled by yki^{S168A.UAS.EGFP,Tag:HA}

(Yeom et al., 2020)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PU

(Guitart et al., 2013)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal, with Scer\GAL4^repo.PU

(Guitart et al., 2013)

lethal | heat sensitive, with Scer\GAL4^Act5C.PU

(Guitart et al., 2013)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

short lived, with Scer\GAL4^Mef2.PU

(Guitart et al., 2013)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^nub-AC-62

(Guitart et al., 2013)

visible, with Scer\GAL4^ptc-559.1

(Guitart et al., 2013)

Phenotype Manifest In

anterior crossvein, with Scer\GAL4^ptc-559.1

(Guitart et al., 2013)

first posterior wing cell, with Scer\GAL4^ptc-559.1

(Guitart et al., 2013)

wing, with Scer\GAL4^nub-AC-62

(Guitart et al., 2013)

Detailed Description

Statement

Reference

1.4% of pupae expressing Aats-ser^GD12798 under the control of Scer\GAL4^Act5C.PU hatch successfully at 25[o]C. No adults hatch at 29[o]C.

Lactate concentration is increased compared to wild type in larvae expressing Aats-ser^GD12798 under the control of Scer\GAL4^Act5C.PU. Glycogen concentration is decreased.

Animals expressing Aats-ser^GD12798 under the control of Scer\GAL4^nub-AC-62 (in the presence of Dcr-2^Scer\UAS.cDa to enhance RNAi efficiency) show tissue damage in the wing, with a penetrance of100% at 25[o]C and 29[o]C.

Partial or total loss of the anterior crossvein is seen in 15% of flies expressing Aats-ser^GD12798 under the control of Scer\GAL4^ptc-559.1 at 29[o]C. The L3-L4 intervein region is significantly reduced in area compared to wild type, with the reduction being due to a decrease in cell number and not in cell size.

Flies expressing Aats-ser^GD12798 under the control of Scer\GAL4^Mef2.PU have a shortened lifespan compared to controls (half-life is reduced from 22 to 6 days). The mutant adults have reduced climbing ability compared to controls.

(Guitart et al., 2013)

Adults expressing Aats-ser^GD12798 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in early pupal lethality or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12798

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Scer\GAL4^GMR.PFa, SerRS-m^GD12798, yki^{S168A.UAS.EGFP,Tag:HA} has lethal - all die before end of P-stage phenotype

(Yeom et al., 2020)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v41928

w¹¹¹⁸; P{GD12798}v41928

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12798

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Aats-ser^GD12798

SerRS-m^GD12798

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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