FlyBase Allele Report: Dmel\Ube3a[GD450]

General Information

Symbol

Dmel\Ube3a^GD450

Species

D. melanogaster

Name

FlyBase ID

FBal0198772

Feature type

allele

Associated gene

Dmel\Ube3a

Associated Insertion(s)

Carried in Construct

P{GD450}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD450}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD450

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of intellectual disability

CEA

(Chakraborty et al., 2015)

model of Angelman syndrome

CEA

(Gossan et al., 2014)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal learning | adult stage, with Scer\GAL4^elav.PU

(Chakraborty et al., 2015)

abnormal learning | adult stage, with Scer\GAL4^ey-OK107

(Chakraborty et al., 2015)

abnormal learning | adult stage, with Scer\GAL4^Tab2-201Y

(Chakraborty et al., 2015)

abnormal neuroanatomy, with Scer\GAL4²²¹

(Lu et al., 2009)

abnormal neuroanatomy, with Scer\GAL4⁴⁷⁷, Ube3a^Δ15-8

(Lu et al., 2009)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav.PU

(Li et al., 2016)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^RapGAP1-OK6

(Li et al., 2016)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^GMR.PU

(Iyer et al., 2016)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing Ube3a^GD450 under the control of Scer\GAL4^GMR.PU leads to a subtle rough eye phenotype.

(Iyer et al., 2016)

Expression of Ube3a^GD450 driven by Scer\GAL4^elav.PU or Scer\GAL4^RapGAP1-OK6 (but not Scer\GAL4^C57) leads to a significant increase in total boutons (and satellite boutons) at the neuromuscular junction, compared to wild type third instar larvae.

(Li et al., 2016)

Adults expressing Ube3a^GD450 under the control of either Scer\GAL4^elav.PU, Scer\GAL4^Tab2-201Y or Scer\GAL4^ey-OK107 show learning defects (significantly reduced performance in a aversive phototaxis suppression assay compared to controls).

(Chakraborty et al., 2015)

Adults expressing Ube3a^GD450 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression of As^GD450 in ddaC neurons under the control of Scer\GAL4⁴⁷⁷ in a As^Δ15-8 heterozygous background results in fewer terminal dendritic branches.

Expression of As^GD450 in ddaE and ddaF neurons under the control of Scer\GAL4²²¹ decreases the number of dendritic branches.

(Lu et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v45875

w¹¹¹⁸; P{GD450}v45875

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

As^GD450

Ube3a^GD450

(Singh et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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