FlyBase Allele Report: Dmel\BRWD3[GD10174]

General Information

Symbol

Dmel\BRWD3^GD10174

Species

D. melanogaster

Name

FlyBase ID

FBal0198819

Feature type

allele

Associated gene

Dmel\BRWD3

Associated Insertion(s)

Carried in Construct

P{GD10174}

Also Known As

UAS-dBRWD3-dsRNA

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10174}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10174

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^ppk.PU

(Chen et al., 2015)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

visible, with Scer\GAL4^ey-OK107

(Chen et al., 2015)

Phenotype Manifest In

arista, with Scer\GAL4^dpp.PU

(Chen et al., 2015)

eye, with Scer\GAL4^ey-OK107

(Chen et al., 2015)

larval multidendritic class IV neuron, with Scer\GAL4^ppk.PU

(Chen et al., 2015)

Detailed Description

Statement

Reference

Flies expressing BRWD3^GD10174 under the control of Scer\GAL4^ey-OK107 exhibit a rough eye phenotype.

When BRWD3^GD10174 is expressed under the control of Scer\GAL4^ppk.PU the class IV dendritic arborization (da) sensory neurons have fewer dendritic branches compared with controls.

Expression of BRWD3^GD10174 under the control of Scer\GAL4^dpp.PU results in impaired development of the arista.

(Chen et al., 2015)

Adults expressing BRWD3^GD10174 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of BRWD3^GD10174 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD10174} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or early pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10174

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

BRWD3^GD10174, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by His3.3B^GD4749, Scer\GAL4^ey-OK107

(Chen et al., 2015)

BRWD3^GD10174, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by Hira^NIG.12153R, Scer\GAL4^ey-OK107

(Chen et al., 2015)

BRWD3^GD10174, Scer\GAL4^ey-OK107 has visible phenotype, suppressible by yem^GD12684, Scer\GAL4^ey-OK107

(Chen et al., 2015)

NOT suppressed by

Statement

Reference

BRWD3^GD10174, Scer\GAL4^ey-OK107 has visible phenotype, non-suppressible by XNP^KK103859, Scer\GAL4^ey-OK107

(Chen et al., 2015)

Phenotype Manifest In

Suppressed by

Statement

Reference

BRWD3^GD10174, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by His3.3B^GD4749, Scer\GAL4^ey-OK107

(Chen et al., 2015)

BRWD3^GD10174, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by Hira^NIG.12153R, Scer\GAL4^ey-OK107

(Chen et al., 2015)

BRWD3^GD10174, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by yem^GD12684, Scer\GAL4^ey-OK107

(Chen et al., 2015)

BRWD3^GD10174, Scer\GAL4^ey-OK107 has eye phenotype, suppressible by XNP^KK103859, Scer\GAL4^ey-OK107

(Chen et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of His3.3B^GD4749 suppresses the rough eye phenotype seen when BRWD3^GD10174 is expressed under the control of Scer\GAL4^ey-OK107. The reduction in the number of dendritic branches seen when BRWD3^GD10174 is expressed under the control of Scer\GAL4^ppk.PU is also suppressed by His3.3B^GD4749.

Expression of His3.3B^GD4749 suppresses the arista defects seen when BRWD3^GD10174 is expressed under the control of Scer\GAL4^dpp.PU.

Expression of Hira^NIG.12153R suppresses the rough eye phenotype seen when BRWD3^GD10174 is expressed under the control of Scer\GAL4^ey-OK107.

Expression of yem^GD12684 suppresses the rough eye phenotype seen when BRWD3^GD10174 is expressed under the control of Scer\GAL4^ey-OK107.

Expression of XNP^KK103859 does not suppress the rough eye phenotype seen when BRWD3^GD10174 is expressed under the control of Scer\GAL4^ey-OK107.

(Chen et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v40209

w¹¹¹⁸; P{GD10174}v40209

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10174

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

BRWD3^GD10174

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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