FlyBase Allele Report: Dmel\Glo1[GD12888]

General Information

Symbol

Dmel\Glo1^GD12888

Species

D. melanogaster

Name

FlyBase ID

FBal0201739

Feature type

allele

Associated gene

Dmel\Glo1

Associated Insertion(s)

Carried in Construct

P{GD12888}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12888}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12888

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | nutrition conditional, with Scer\GAL4^Cg.PA

(Garrido et al., 2015)

decreased size | adult stage, with Scer\GAL4^C-765

(Spannl et al., 2020)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

wing, with Scer\GAL4^C-765

(Spannl et al., 2020)

Detailed Description

Statement

Reference

When fed on a low carbohydrate diet (0% sucrose supplement), a moderate developmental delay is observed for Scer\GAL4^Cg.PA Glo1^GD12888 (plus Dcr-2^Scer\UAS.cDa) animals. This delay is slightly prolonged when fed a sucrose supplemented diet.

Cell size within MARCM clones expressing only Glo1^GD12888 is insensitive to sucrose supplementation.

(Garrido et al., 2015)

Adults expressing Glo1^GD12888 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 50-60% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 10-20% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12888

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Glo1^GD12888, Scer\GAL4^Cg.PA has abnormal developmental rate | nutrition conditional phenotype, enhanceable by FASN1^GD14739, Scer\GAL4^Cg.PA

(Garrido et al., 2015)

Enhancer of

Statement

Reference

Glo1^GD12888, Scer\GAL4^Cg.PA is an enhancer of abnormal developmental rate | nutrition conditional phenotype of FASN1^GD14739, Scer\GAL4^Cg.PA

(Garrido et al., 2015)

Other

Statement

Reference

Df(2L)FASN^Δ24-23, Glo1^GD12888, Scer\GAL4^Act5C.PP has decreased cell size | somatic clone phenotype

(Garrido et al., 2015)

FASN1^GD14739, Glo1^GD12888, Scer\GAL4^Cg.PA has lethal | nutrition conditional phenotype

(Garrido et al., 2015)

Phenotype Manifest In

Other

Statement

Reference

Df(2L)FASN^Δ24-23, Glo1^GD12888, Scer\GAL4^Act5C.PP has nucleus | somatic clone phenotype

(Garrido et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Animals co-expressing Glo1^GD12888 and FASN1^GD14739 (plus Dcr-2^Scer\UAS.cDa) via Scer\GAL4^Cg.PA exhibit a high rate of larval lethality and developmental delay that dramatically increases concurrently with sucrose concentration.

Cells within Df(2L)FASN^Δ24-23 MARCM clones expressing Glo1^GD12888 (plus Dcr-2^Scer\UAS.cDa) exhibit an extreme size reduction accompanied by a severe decrease in nucleus size in larvae fed a low carbohydrate diet. Furthermore, these clonal cells cannot be observed when larvae were fed 20% sucrose supplemented diet, suggesting that these cells are eliminated during development.

(Garrido et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v26832

w¹¹¹⁸; P{GD12888}v26832

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12888

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG1707^GD12888

Glo1^GD12888

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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