FlyBase Allele Report: Dmel\FASN1[GD14739]

General Information

Symbol

Dmel\FASN1^GD14739

Species

D. melanogaster

Name

FlyBase ID

FBal0203970

Feature type

allele

Associated gene

Dmel\FASN1

Associated Insertion(s)

Carried in Construct

P{GD14739}

Also Known As

FASN^CG3523-RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14739}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14739

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | nutrition conditional, with Scer\GAL4^Cg.PA

(Garrido et al., 2015)

abnormal locomotor behavior | adult stage, with Scer\GAL4^Mef2.PU

(Garrido et al., 2015)

decreased cell size | somatic clone | nutrition conditional, with Scer\GAL4^Act5C.PP

(Garrido et al., 2015)

lethal - all die during first instar larval stage, with Scer\GAL4^da.PU

(Garrido et al., 2015)

short lived, with Scer\GAL4^da.PU

(Devilliers et al., 2021)

viable, with Scer\GAL4^Cg.PA

(Garrido et al., 2015)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^Mef2.PU

(Garrido et al., 2015)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^sal.BO

(Parvy et al., 2012)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^Bx-MS1096

(Friedman et al., 2011)

Phenotype Manifest In

embryonic/larval hemolymph | third instar larval stage, with Scer\GAL4^Adh.PF

(Palm et al., 2012)

wing, with Scer\GAL4^Bx-MS1096

(Friedman et al., 2011)

Detailed Description

Statement

Reference

The expression of FASN1^GD14739 from the onset of adulthood, under the combined control of Scer\GAL4^da.PU and Gal80[ts], leads to decreased lifespan.

(Devilliers et al., 2021)

When fed on a low carbohydrate diet (0% sucrose supplement), no developmental delay is observed for Scer\GAL4^Cg.PA FASN1^GD14739 (plus Dcr-2^Scer\UAS.cDa) animals. In contrast, when fed a 10% or 20% sucrose supplemented diet, these animals exhibit a 2-8 day delay, respectively.

The size of cells in flip-out Scer\GAL4^Act5C.PP FASN1^GD14739 (plus Dcr-2^Scer\UAS.cDa) clones is almost normal in larvae fed a low carbohydrate diet, but is drastically reduced in larvae fed a 20% sucrose supplemented diet.

(Garrido et al., 2015)

Expression of FASN1^GD14739 under the control of Scer\GAL4^sal.BO (in combination with a Dicer-2 transgene to enhance RNAi efficiency) does not result in larval lethality at the L2/L3 transition.

(Wicker-Thomas et al., 2015)

Expression of CG3523^GD14739 in the gut under the control of Scer\GAL4^Adh.PF reduces larval hemolymph diacylglycerol (DAG) by 30%.

(Palm et al., 2012)

Expression of CG3523^GD14739 under the control of Scer\GAL4^Bx-MS1096 results in slight curling of the wing.

(Friedman et al., 2011)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14739

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

FASN1^GD14739, Scer\GAL4^Cg.PA has abnormal developmental rate | nutrition conditional phenotype, enhanceable by Glo1^GD12888, Scer\GAL4^Cg.PA

(Garrido et al., 2015)

Suppressed by

Statement

Reference

FASN1^GD14739, Scer\GAL4^Cg.PA has abnormal developmental rate | nutrition conditional phenotype, suppressible | partially by Glo1^UAS.cGa, Scer\GAL4^Cg.PA

(Garrido et al., 2015)

Enhancer of

Statement

Reference

FASN1^GD14739, Scer\GAL4^Cg.PA is an enhancer of abnormal developmental rate | nutrition conditional phenotype of Glo1^GD12888, Scer\GAL4^Cg.PA

(Garrido et al., 2015)

NOT Enhancer of

Statement

Reference

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-enhancer of abnormal neuroanatomy | adult stage phenotype of mir-210^Δ

(Weigelt et al., 2019)

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-enhancer of decreased cell number | adult stage phenotype of mir-210^Δ

(Weigelt et al., 2019)

Suppressor of

Statement

Reference

FASN1^GD14739/Scer\GAL4^GMR.PU is a suppressor | partially of abnormal neurophysiology | adult stage phenotype of mir-210^Δ

(Weigelt et al., 2019)

NOT Suppressor of

Statement

Reference

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-suppressor of abnormal neuroanatomy | adult stage phenotype of mir-210^Δ

(Weigelt et al., 2019)

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-suppressor of decreased cell number | adult stage phenotype of mir-210^Δ

(Weigelt et al., 2019)

Other

Statement

Reference

FASN1^GD14739, Glo1^GD12888, Scer\GAL4^Cg.PA has lethal | nutrition conditional phenotype

(Garrido et al., 2015)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-enhancer of eye photoreceptor cell phenotype of mir-210^Δ

(Weigelt et al., 2019)

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-enhancer of rhabdomere of eye photoreceptor cell phenotype of mir-210^Δ

(Weigelt et al., 2019)

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-enhancer of vacuole | adult stage phenotype of mir-210^Δ

(Weigelt et al., 2019)

Suppressor of

Statement

Reference

Scer\GAL4^da.PU/FASN1^GD14739 is a suppressor | partially of larval fat cell | somatic clone | third instar larval stage phenotype of Pten¹¹⁷

(Devilliers et al., 2021)

FASN1^GD14739/Scer\GAL4^GMR.PU is a suppressor | partially of retina phenotype of mir-210^Δ

(Weigelt et al., 2019)

NOT Suppressor of

Statement

Reference

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-suppressor of eye photoreceptor cell phenotype of mir-210^Δ

(Weigelt et al., 2019)

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-suppressor of rhabdomere of eye photoreceptor cell phenotype of mir-210^Δ

(Weigelt et al., 2019)

FASN1^GD14739/Scer\GAL4^GMR.PU is a non-suppressor of vacuole | adult stage phenotype of mir-210^Δ

(Weigelt et al., 2019)

Additional Comments

Genetic Interactions

Statement

Reference

Flies co-expressing GlyS^GD12070 and FASN1^GD14739 (plus Dcr-2^Scer\UAS.cDa) in both muscles and fat body (via Scer\GAL4^Cg.PA and Scer\GAL4^Mef2.PU) exhibit a prominent reduction in body weight.

Animals co-expressing Glo1^GD12888 and FASN1^GD14739 (plus Dcr-2^Scer\UAS.cDa) via Scer\GAL4^Cg.PA exhibit a high rate of larval lethality and developmental delay that dramatically increases concurrently with sucrose concentration. Conversely, co-expression of Glo1^Scer\UAS.cGa partially suppresses the strong developmental delay of Scer\GAL4^Cg.PA FASN1^GD14739 (plus Dcr-2^Scer\UAS.cDa) animals grown on a sucrose-supplemented diet.

(Garrido et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v29349

w¹¹¹⁸; P{GD14739}v29349/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14739

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG3523^GD14739

FASN1^GD14739

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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