FlyBase Allele Report: Dmel\Vps13D[GD10464]

General Information

Symbol

Dmel\Vps13D^GD10464

Species

D. melanogaster

Name

FlyBase ID

FBal0203179

Feature type

allele

Associated gene

Dmel\Vps13D

Associated Insertion(s)

Carried in Construct

P{GD10464}

Also Known As

Vps13D RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD10464}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD10464

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell size | pupal stage, with Scer\GAL4^NP0001

(Anding et al., 2018)

increased cell size | pupal stage | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Anding et al., 2018)

lethal, with Scer\GAL4^Act5C.PI

(Anding et al., 2018)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

midgut | pupal stage, with Scer\GAL4^NP0001

(Anding et al., 2018)

midgut | pupal stage | somatic clone, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Anding et al., 2018)

mitochondrion | pupal stage, with Scer\GAL4^NP0001

(Anding et al., 2018)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

The cell size in pupal (2hr after pupariation) midgut somatic clones expressing Vps13D^GD10464 under the control of Scer\GAL4^{Scer\FRT.Rnor\Cd2.Act5C} is significantly increased and the cells contain fewer Atg8a puncta compared to control cells.

Expression of Vps13D^GD10464 under the control of Scer\GAL4^{Myo31DF-NP0001} also leads to increase midgut cell size along with enlargement of mitochondria (but their cristae are juxtaposed and appear intact) in the pupal intestines at 2hr after puparium formation.

(Anding et al., 2018)

Adults expressing CG32113^GD10464 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 80-90% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10464

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^NP0001, Vps13D^GD10464 has increased cell size | pupal stage phenotype, suppressible by Marf^GD11094, Scer\GAL4^NP0001

(Anding et al., 2018)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP0001, Vps13D^GD10464 has midgut | pupal stage phenotype, suppressible by Marf^GD11094, Scer\GAL4^NP0001

(Anding et al., 2018)

Scer\GAL4^NP0001, Vps13D^GD10464 has mitochondrion | pupal stage phenotype, suppressible by Marf^GD11094, Scer\GAL4^NP0001

(Anding et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

The increased midgut cell size along with their enlarged mitochondria observed in pupae (2hr after puparium formation) expressing Vps13D^GD10464 under the control of Scer\GAL4^{Myo31DF-NP0001} is rescued by co-expression of Marf^GD11094.

(Anding et al., 2018)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v41792

w¹¹¹⁸; P{GD10464}v41792

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

10464

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG32113^GD10464

Vps13D^GD10464

Name Synonyms

Secondary FlyBase IDs

References (9)

Report Sections

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