FlyBase Allele Report: Dmel\Isha[GD11266]

General Information

Symbol

Dmel\Isha^GD11266

Species

D. melanogaster

Name

FlyBase ID

FBal0204202

Feature type

allele

Associated gene

Dmel\Isha

Associated Insertion(s)

Carried in Construct

P{GD11266}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD11266}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD11266

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of syndrome

CEA

(Fliedner et al., 2020)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

abnormal learning | adult stage, with Scer\GAL4^elav-C155

(Fliedner et al., 2020)

abnormal learning | adult stage, with Scer\GAL4^Mef2.247

(Fliedner et al., 2020)

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PLu

(Fliedner et al., 2020)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Fliedner et al., 2020)

abnormal short-term memory | adult stage, with Scer\GAL4^elav-C155

(Fliedner et al., 2020)

abnormal short-term memory | adult stage, with Scer\GAL4^Mef2.247

(Fliedner et al., 2020)

partially lethal, with Scer\GAL4^Act5C.PU

(Bag et al., 2022)

partially lethal, with Scer\GAL4^Tub.PU

(Bag et al., 2022)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

some die during P-stage, with Scer\GAL4^Act5C.PU

(Bag et al., 2022)

some die during P-stage, with Scer\GAL4^Tub.PU

(Bag et al., 2022)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^{l(3)31-1-31-1}

(Bag et al., 2022)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^Mef2.PU

(Bag et al., 2022)

viable, with Scer\GAL4^Ser.PU

(Bag et al., 2022)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

Detailed Description

Statement

Reference

Expressing CG4266^GD11266 under the control of Scer\GAL4^elav-C155 leads to the third instar larval NMJ spanning a larger area, and showing more boutons and more active zones, as compared to controls. It also leads to impaired learning and short-term memory in courtship conditioning paradigm assays.

Expressing CG4266^GD11266 under the control of Scer\GAL4^elav.PLu leads to impaired climbing activity in adults.

Expressing CG4266^GD11266 under the control of Scer\GAL4^Mef2.247 leads to impaired learning and short-term memory in courtship conditioning paradigm assays.

(Fliedner et al., 2020)

Adults expressing CG4266^GD11266 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in extra bristles on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 60-70% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in mild thorax closure defects, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 may result in an enlarged notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development