FlyBase Allele Report: Dmel\Vps39[GD12152]

General Information

Symbol

Dmel\Vps39^GD12152

Species

D. melanogaster

Name

FlyBase ID

FBal0205346

Feature type

allele

Associated gene

Dmel\Vps39

Associated Insertion(s)

Carried in Construct

P{GD12152}

Also Known As

GD12152, UAS-VPS39 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12152}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12152

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal body color, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

increased cell size, with Scer\GAL4^pros.PU

(Lőrincz et al., 2019)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

autolysosome | larval stage | somatic clone | calorie restriction conditional, with Scer\GAL4^Act.PU

(Lőrincz et al., 2019)

autolysosome | somatic clone | wandering third instar larval stage | nutrition conditional, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Takáts et al., 2014)

autophagosome | larval stage | somatic clone | calorie restriction conditional, with Scer\GAL4^Act.PU

(Lőrincz et al., 2019)

embryonic/larval fat body | somatic clone | calorie restriction conditional, with Scer\GAL4^Act.PU

(Lőrincz et al., 2019)

embryonic/larval fat body | somatic clone | wandering third instar larval stage | nutrition conditional, with Scer\GAL4^{FRT.Rnor\Cd2.Act5C}

(Takáts et al., 2014)

embryonic/larval garland cell | larval stage, with Scer\GAL4^unspecified

(Lőrincz et al., 2016)

garland cell, with Scer\GAL4^pros.PU

(Lőrincz et al., 2019)

late endosome, with Scer\GAL4^pros.PU

(Lőrincz et al., 2019)

late endosome | larval stage, with Scer\GAL4^unspecified

(Lőrincz et al., 2016)

lysosome | larval stage | somatic clone | calorie restriction conditional, with Scer\GAL4^Act.PU

(Lőrincz et al., 2019)

mesonotum, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

vacuole | larval stage, with Scer\GAL4^unspecified

(Lőrincz et al., 2016)

Detailed Description

Statement

Reference

Garland cells expressing Vps39^GD12152 under the control of Scer\GAL4^pros.PU are enlarged and show enlarged late endosomes (Rab7-positive puncta), but not early endosomes (Rab5-positive puncta).

Under starvation, larval fat body clones expressing Vps39^GD12152 under the control of Scer\GAL4^Act.PU lack the bright lysosome (Lamp-positive) and autolysosome (mCh-Atg8a-positive) puncta normally observed in controls, showing instead only 'clouds' of many faint autophagosome puncta (endogenous Atg8a-positive). These clones also show enlarged Rab7-positive puncta, which represent both autophagosomes and lysosomes in these cells.

(Lőrincz et al., 2019)

Expression of car^GD1397 under the control of Scer\GAL4^unspecified results in larval garland nephrocytes that exhibit enlargement of late endosomes and enlarged vacuoles with multiple dense cores, as compared to controls.

(Lőrincz et al., 2016)

Fat body cell clones expressing Vps39^GD12152 under the control of Scer\GAL4^{Scer\FRT.Rnor\CD2.Act5C} exhibit impaired starvation-induced autophagy.

(Takáts et al., 2014)

Adults expressing Vps39^GD12152 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Vps39^GD12152 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD12152} insertion line used.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0%, 30-40% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0%, 10-20% or 80-90% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0%, 10-20% or 20-30% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12152

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^nSyb.PU/Vps39^GD12152 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of sky¹/sky²

(Fernandes et al., 2014)

Phenotype Manifest In

Suppressor of

Statement

Reference

Vps39^GD12152/Scer\GAL4^hh-Gal4 is a suppressor of phenotype of l(2)gd1^d7

(Schneider et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

The increase in excitatory junctional current amplitude at the neuromuscular junction which is seen in sky¹/sky² larvae is suppressed if they are also expressing Vps39^GD12152 under the control of Scer\GAL4^nSyb.PU. However, the increased synaptic vesicle trafficking to endosomes seen at the neuromuscular junction in the sky¹/sky² larvae is not suppressed.

(Fernandes et al., 2014)

Scer\GAL4^hh-Gal4-mediated expression of Vps39^GD12152 suppresses the ectopic N activation phenotype (ectopic expression of wg) in the posterior of l(2)gd1^d7 wing discs.

(Schneider et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v40427

w¹¹¹⁸; P{GD12152}v40427

v46200

w¹¹¹⁸; P{GD12152}v46200

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12152

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

CG7146^GD12152

Vps39^GD12152

Vps39[GD12152]

(Takáts et al., 2014)

dVps39^RNAi

(Fernandes et al., 2014)

vps39^RNAi

(Lőrincz et al., 2019)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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