FlyBase Allele Report: Dmel\trbd[GD14218]

General Information

Symbol

Dmel\trbd^GD14218

Species

D. melanogaster

Name

FlyBase ID

FBal0206230

Feature type

allele

Associated gene

Dmel\trbd

Associated Insertion(s)

Carried in Construct

P{GD14218}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD14218}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD14218

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates spinocerebellar ataxia 6

modeled by Hsap\CACNA1A^{UAS.αACT.Q70}

(Tsou et al., 2015)

ameliorates Machado-Joseph disease

modeled by Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}

(Voßfeldt et al., 2012)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | adult stage, with Scer\GAL4^elav.PU

(Tsou et al., 2012)

abnormal locomotor behavior | adult stage, with Scer\GAL4^sqh.PW

(Tsou et al., 2012)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

partially lethal, with Scer\GAL4^sqh.PW

(Tsou et al., 2012)

short lived, with Scer\GAL4^elav.PU

(Tsou et al., 2012)

short lived, with Scer\GAL4^sqh.PW

(Tsou et al., 2012)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The majority of Scer\GAL4^sqh.PW, trbd^GD14218 animals die as pharates or young adults. All are dead by 40 days. Adults are slower than controls.

Scer\GAL4^elav.PU, trbd^GD14218 adults are slower than controls and are dead by 40 days.

(Tsou et al., 2012)

Expression of trbd^GD14218 under the control of Scer\GAL4^bbg-C96 does not result in a wing phenotype.

Expression of trbd^GD14218 under the control of Scer\GAL4^dpp.PU does not result in a bristle phenotype.

(Zhang et al., 2012)

Adults expressing trbd^GD14218 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14218

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.PU/trbd^GD14218, Scer\GAL80^ts.αTub84B is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Suppressor of

Statement

Reference

trbd^GD14218, Scer\GAL4^GMR.PU is a suppressor of abnormal neuroanatomy | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

trbd^GD14218, Scer\GAL4^GMR.long is a suppressor of visible phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.PU/trbd^GD14218, Scer\GAL80^ts.αTub84B is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Phenotype Manifest In

Suppressor of

Statement

Reference

trbd^GD14218, Scer\GAL4^GMR.PU is a suppressor of retina | adult stage | progressive phenotype of Hsap\CACNA1A^{UAS.αACT.Q70}, Scer\GAL4^GMR.PU

(Tsou et al., 2015)

trbd^GD14218, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\MAPT^R406W.UAS, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

trbd^GD14218, Scer\GAL4^GMR.long is a suppressor of eye phenotype of Hsap\ATXN3^{tr.Q78.UAS.Tag:HA}, Scer\GAL4^GMR.long

(Voßfeldt et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of trbd^GD14218 suppresses the progressive retinal degeneration (at day 7 but not day 14) seen in fly eyes with Hsap\CACNA1A^{Scer\UAS.αACT.Q70} driven by Scer\GAL4^GMR.PU.

(Tsou et al., 2015)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v24030

w¹¹¹⁸; P{GD14218}v24030

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

14218

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

CG9448^GD14218

trbd^GD14218

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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