FlyBase Allele Report: Dmel\Cbl[GD12111]

General Information

Symbol

Dmel\Cbl^GD12111

Species

D. melanogaster

Name

FlyBase ID

FBal0206522

Feature type

allele

Associated gene

Dmel\Cbl

Associated Insertion(s)

Carried in Construct

P{GD12111}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12111}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12111

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal oxidative stress response, with Scer\GAL4^elav.PU

(Yu et al., 2012)

abnormal oxidative stress response | male limited, with Scer\GAL4^Ilp2.PR

(Yu et al., 2012)

increased body size | male limited, with Scer\GAL4^elav.PU

(Yu et al., 2012)

increased body size | male limited, with Scer\GAL4^Ilp2.PR

(Yu et al., 2012)

short lived, with Scer\GAL4^elav.PU

(Yu et al., 2012)

short lived | male limited, with Scer\GAL4^Ilp2.PR

(Yu et al., 2012)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Neuronal expression of Cbl^GD12111 using Scer\GAL4^elav.PU leads to an approximately 12% increase in body weight of male adult flies compared with controls.

Neuronal expression of Cbl^GD12111 using Scer\GAL4^elav.PU significantly reduces the lifespan of both male and female flies compared with controls. Consistent with their shorter lifespan, both male and female Scer\GAL4^elav.PU>Cbl^GD12111 flies exhibit reduced tolerance to oxidative stress in the form of paraquat treatment relative to controls.

Males expressing Cbl^GD12111 under the control of Scer\GAL4^Ilp2.PR display an approximately 9% increase in body weight and exhibit a significant shortening of the lifespan compared with controls.

Males expressing Cbl^GD12111 under the control of Scer\GAL4^Ilp2.PR show increased sensitivity to paraquat compared with controls, indicating reduced tolerance to oxidative stress.

Flies expressing Cbl^GD12111 using Scer\GAL4^Ilp2.PR show no alterations in the morphology and number of insulin producing cells compared to controls.

(Yu et al., 2012)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 10-20% of the Scer\GAL4^pnr-MD237 expression domain.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12111

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cbl^GD12111, Scer\GAL4^elav.PU has increased body size | male limited phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^elav.PU

(Yu et al., 2012)

Cbl^GD12111, Scer\GAL4^Ilp2.PR has increased body size | male limited phenotype, suppressible by Egfr^DN.UAS.cBa, Scer\GAL4^Ilp2.PR

(Yu et al., 2012)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Neuronal Egfr^{DN.Scer\UAS.cBa} expression using Scer\GAL4^elav.PU suppresses the Cbl^GD12111-induced increase in body weight in males.

Expression of Egfr^{DN.Scer\UAS.cBa} in insulin producing cells under the control of Scer\GAL4^Ilp2.PR suppresses the Cbl^GD12111-induced increase in body weight in males.

(Yu et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

VDRC

v22335

w¹¹¹⁸; P{GD12111}v22335/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12111

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Cbl^GD12111

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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