FlyBase Allele Report: Dmel\Hsc70-3[GD6484]

General Information

Symbol

Dmel\Hsc70-3^GD6484

Species

D. melanogaster

Name

FlyBase ID

FBal0207331

Feature type

allele

Associated gene

Dmel\Hsc70-3

Associated Insertion(s)

Carried in Construct

P{GD6484}

Also Known As

UAS-Hsc70-3RNAi, UAS-Bip^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD6484}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD6484

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell number | larval stage | somatic clone, with Scer\GAL4^Act.PU

(Zheng et al., 2023)

decreased size | spermatogenesis, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

lethal, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal | paternal effect, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

lethal - all die during P-stage, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

eye-antennal disc | somatic clone, with Scer\GAL4^Act.PU

(Zheng et al., 2023)

spermatocyte cyst, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

spermatozoon | absent, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

testis, with Scer\GAL4^VP16.bam

(Azuma et al., 2021)

Detailed Description

Statement

Reference

Adults expressing Hsc70-3^GD6484 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in lethality before the pupal stage.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development